SUMO pathway is required for ribosome biogenesis

Abstract

Ribosomes, acting as the cellular factories for protein production, are essential for all living organisms. Ribosomes are composed of both proteins and RNAs and are established through the coordination of several steps, including transcription, maturation of ribosomal RNA (rRNA), and assembly of ribosomal proteins. In particular, diverse factors required for ribosome biogenesis, such as transcription factors, small nucleolar RNA (snoRNA)-associated proteins, and assembly factors, are tightly regulated by various post-translational modifications. Among these modifications, small ubiquitin-related modifier (SUMO) targets lots of proteins required for gene expression of ribosomal proteins, rRNA, and snoRNAs, rRNA processing, and ribosome assembly. The tight control of SUMOylation affects functions and locations of substrates. This review summarizes current studies and recent progress of SUMOylation-mediated regulation of ribosome biogenesis. [

Transcriptomic Profiling of Fusarium pseudograminearum in Response to Carbendazim, Pyraclostrobin, Tebuconazole, and Phenamacril

“…Additionally, several proteins involved in ribosome biogenesis and protein assembly showed higher expression levels in CAR groups, such as FPSE_01828 ( NUG1 ) and FPSE_00408 ( RRB1 ). It is known that ribosome biogenesis is a very tightly regulated process, and it is more closely linked to other cellular activities such as growth and division [ 53 ]. In the PHE group, FPSE_09193 encoding chitin synthase 8 was significantly up-regulated.…”

Auto-sumoylation of the Ubc9 E2 SUMO-conjugating Enzyme Extends Cellular Lifespan

“…Energy metabolism genes, including tricarboxylic acid (TCA) cycle, ATP biosynthesis, and respiratory electron transport chain-related genes, were speci cally down-regulated in nascent ulp2Δ, whereas ribosomal proteins and SUMO-regulated ribosome biogenesis factors 24 were heavily enriched among the genes with a strong increase in expression. In direct contrast to this pattern, genes required for translation, including those involved in rRNA maturation, ribosome assembly and nuclear export, and translation factors, were down-regulated evolved ulp2Δ 500G (Uba2 C162S ) cells, while genes for energy reserve and cellular carbohydrate metabolic processes were upregulated in this line.…”

“…7e). SUMO is known to act as a key regulatory factor in both of these pathways 24 . Our data reveal that multiple translation initiation and termination factors, as well as ribosomal proteins, are only detectably sumoylated when Ubc9 can be auto-sumoylated, and the same is true for multiple glycolytic and TCA enzymes.…”

Yeast Small Ubiquitin-Like Modifier (SUMO) Protease Ulp2 is Involved in RNA Splicing

“…In the budding yeast, Saccharomyces cerevisiae , Small Ubiquitin-like Modifier (SUMO) proteins are conjugated to target proteins through the sequential action of E1 activating enzymes, E2 conjugating enzymes, and E3 ligases ( Melchior, 2000 ). This post-translational modification plays pivotal roles in various cellular processes, including transcriptional regulation, DNA repair, translation, and cell cycle progression ( Choi et al, 2021 ; Ryu and Hochstrasser, 2021 ; Ryu, 2022 ). A subset of SUMO conjugates undergoes deSUMOylation catalyzed by the proteases, Ulp1 and Ulp2 ( Ryu et al, 2019 ).…”