2012
DOI: 10.1111/j.1469-0691.2012.03809.x
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Super-paramagnetic iron oxide nanoparticles for use in extrapulmonary tuberculosis diagnosis

Abstract: The limited sensitivity of serological tests for mycobacterial antigens has encouraged the development of a nanoparticle probe specific for the extrapulmonary form of Mycobacterium tuberculosis (Mtb). We developed an innovative probe comprised of super-paramagnetic iron oxide (SPIO) nanoparticles conjugated with Mtb surface antibody (MtbsAb-nanoparticles) to provide ultrasensitive imaging of biomarkers involved in extrapulmonary Mtb infection. MtbsAb-nanoparticles were significantly conjugated with Mtb bacilli… Show more

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Cited by 33 publications
(12 citation statements)
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“…Cell labeling and tracking experiments are commonly performed either by labeling immune cells in vitro and regrafting them or by systemic application of iron oxide particles which are then phagocytosed by macrophages [21]. By using negative iron contrast immunological reactions and bacteria-induced inflammatory dissemination have already been investigated in animal models of Staphylococcus aureus [22-26], Toxoplasma gondii [27], Mycobacterium tuberculosis [28] or LPS-induced sepsis conditions [29,30]. However, only BLI so far was capable of visualizing bacteria directly.…”
Section: Introductionmentioning
confidence: 99%
“…Cell labeling and tracking experiments are commonly performed either by labeling immune cells in vitro and regrafting them or by systemic application of iron oxide particles which are then phagocytosed by macrophages [21]. By using negative iron contrast immunological reactions and bacteria-induced inflammatory dissemination have already been investigated in animal models of Staphylococcus aureus [22-26], Toxoplasma gondii [27], Mycobacterium tuberculosis [28] or LPS-induced sepsis conditions [29,30]. However, only BLI so far was capable of visualizing bacteria directly.…”
Section: Introductionmentioning
confidence: 99%
“…10 Metal-based NPs, for example, have an important application as nano-sized sensors for biological labeling and as vectors for drug delivery. 11,12 However, many metal NPs, such as zinc oxide NPs, have adverse effects on human health because of their high bioreactivity.…”
Section: Introductionmentioning
confidence: 99%
“…Here the nanoparticles are conjugated to anti-tuberculosis antibodies and administered to the subject. The antibodies bind to the bacteria and the nanoparticles act as a contrast agent in an MRI scan, allowing the infected areas to be imaged 39. In cancer diagnosis nanoparticles are acting as contrast agents and have been used in studies of pancreatic cancer, again being conjugated to antibodies specific to cancer cell markers 40.…”
Section: Nanoparticlesmentioning
confidence: 99%