Super-Resolution Imaging in Raman Microscopy

Fujita, Katsumasa

doi:10.1007/978-3-030-21722-8_8

Cited by 4 publications

(4 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…98 Unlike traditional Raman microscopy, which is limited by diffraction to a spatial resolution of approximately half the wavelength of the incident light, SRRM employs various strategies to achieve resolutions beyond this limit, often down to tens of nanometers or even single-digit nanometer scales. 99,100 One common approach in SRRM is to utilize near-field Raman techniques, such as tip-enhanced Raman spectroscopy (TERS), which involves scanning a sharp metallic tip in close proximity to the sample surface. 101 Another approach involves the use of super-resolution optical techniques, such as stimulated emission depletion (STED) microscopy or structured illumination microscopy (SIM), in combination with RS.…”

Section: Different Scanning and Imaging Techniquesmentioning

confidence: 99%

“…Furthermore, advancements in super-resolution imaging restoration techniques and structured illumination methods have contributed to improving spatial resolution, particularly for clinical applications. [98][99][100]105 These advancements enable detailed spectral information retrieval related to specific molecular structures and substances, aiding in precise disease diagnosis such as cancer, infections, and neurodegenerative conditions. Integration of RS with other imaging modalities, like optical coherence tomography (OCT) or fluorescence microscopy, can provide complementary information and enhance spatial resolution in clinical settings.…”

Section: Time and Spatial Resolutionmentioning

confidence: 99%

See 1 more Smart Citation

Unveiling brain disorders using liquid biopsy and Raman spectroscopy

Ranasinghe,

Wang,

Huang

2024

Nanoscale

View full text Add to dashboard Cite

show abstract

Section: Different Scanning and Imaging Techniquesmentioning

confidence: 99%

Section: Time and Spatial Resolutionmentioning

confidence: 99%

Unveiling brain disorders using liquid biopsy and Raman spectroscopy

Ranasinghe,

Wang,

Huang

2024

Nanoscale

View full text Add to dashboard Cite

show abstract

“…Thus, quantitative colocalization analyses have been used between NLO channels throughout this study, serving as a validation of image and statistical analysis, as detailed in the following sections. Also, these techniques can be developed under practical conditions and allow us to perform ultrafast chemical imaging (i.e., 1.5 ms per pixel), surpassing other super-resolution microscopy methods (39), thus paving the way for future exploitation as easy-to-use screening tools.…”

Section: Multimodal Nlo Imaging Unveils Subcellular Chemical Maps Of ...mentioning

confidence: 99%

Noninvasive morpho-molecular imaging reveals early therapy-induced senescence in human cancer cells

Bresci,

Kim,

Ghislanzoni

et al. 2023

Sci. Adv.

View full text Add to dashboard Cite

Anticancer therapy screening in vitro identifies additional treatments and improves clinical outcomes. Systematically, although most tested cells respond to cues with apoptosis, an appreciable portion enters a senescent state, a critical condition potentially driving tumor resistance and relapse. Conventional screening protocols would strongly benefit from prompt identification and monitoring of therapy-induced senescent (TIS) cells in their native form. We combined complementary all-optical, label-free, and quantitative microscopy techniques, based on coherent Raman scattering, multiphoton absorption, and interferometry, to explore the early onset and progression of this phenotype, which has been understudied in unperturbed conditions. We identified TIS manifestations as early as 24 hours following treatment, consisting of substantial mitochondrial rearrangement and increase of volume and dry mass, followed by accumulation of lipid vesicles starting at 72 hours. This work holds the potential to affect anticancer treatment research, by offering a label-free, rapid, and accurate method to identify initial TIS in tumor cells.

show abstract

“…15 Raman imaging is generally hindered by a weak Raman scattering cross-section, resulting in low signal intensity and thus requiring long acquisition times. 16 The Raman scattering cross-section is notably large for a wide variety of semiconductor materials used in the electronic industry and for emerging materials such as carbon-containing materials (i.e., carbon nanotubes and graphene) and 2D transition metal dichalcogenides. A variety of approaches are used to amplify Raman signal, including surface-enhanced Raman spectroscopy (SERS), 17 and tip-enhanced Raman spectroscopy (TERS), which also yields an increase in spatial resolution.…”

Section: Introductionmentioning

confidence: 99%

Investigating the Performances of Wide-Field Raman Microscopy with Stochastic Optical Reconstruction Post-Processing

et al. 2022

View full text Add to dashboard Cite

Super-resolution fluorescence microscopy based on localization algorithms has tremendously impacted the field of imaging by improving the spatial resolution of optical measurements with specific blinking fluorophores and concomitant reduction of acquisition time. In vibrational spectroscopy and imaging, various methods have been developed to surpass the diffraction limit including near-field scattering methods, such as in tip-enhanced Raman and infrared spectroscopies. Although these scanning-probe techniques can provide exquisite spatial resolution, they often require long acquisition times and tedious fabrication of nano-scale scanning probes. Herein, stochastic optical reconstruction microscopy (STORM) protocol is applied on Raman measurements acquired using a wide-field home-built microscopy setup. We explore how the fluctuations of the Raman signal acquired over a series of time-lapse images at specific spectral ranges can be exploited with STORM processing, possibly revealing details with improved spatial resolution, under lower irradiance and with faster acquisition speed that cannot be achieved in point scanning mode over the same field of view. Samples studied here include patterned silicon, polystyrene microspheres on a silicon wafer, and graphene on a silicon/silicon dioxide substrate. The outcome presents an effective way to collect Raman images at selected spectral ranges with spatial resolutions of ∼200 nm over a large field of view under 532 nm excitation together with an acquisition speed improved by two orders of magnitude and under a significantly reduced irradiance compared to confocal laser scanning acquisition.

show abstract

Super-Resolution Imaging in Raman Microscopy

Cited by 4 publications

References 49 publications

Unveiling brain disorders using liquid biopsy and Raman spectroscopy

Unveiling brain disorders using liquid biopsy and Raman spectroscopy

Noninvasive morpho-molecular imaging reveals early therapy-induced senescence in human cancer cells

Investigating the Performances of Wide-Field Raman Microscopy with Stochastic Optical Reconstruction Post-Processing

Contact Info

Product

Resources

About