2022
DOI: 10.1007/s10577-021-09679-w
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Super-resolution microscopy reveals stochastic initiation of replication in Drosophila polytene chromosomes

Abstract: Studying the probability distribution of replication initiation along a chromosome is a huge challenge. Drosophila polytene chromosomes in combination with super-resolution microscopy provide a unique opportunity for analyzing the probabilistic nature of replication initiation at the ultrastructural level. Here, we developed a method for synchronizing S-phase induction among salivary gland cells. An analysis of the replication label distribution in the first minutes of S phase and in the following hours after … Show more

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