Superantigen Presentation by Human Retinal Pigment Epithelial Cells to T Cells is Dependent on CD2–CD58 and CD18–CD54 Molecule Interactions

Jørgensen, A.; Junker, Niels; Kaestel, C G; Liang, Ye; Wiencke, Anne Katrine; Cour, Morten la; Lui, Ge Ming; Ødum, Niels; Nissen, Mogens Holst; Røpke, Carsten

doi:10.1006/exer.2001.1082

Cited by 11 publications

(6 citation statements)

References 30 publications

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“…The functional characteristics of this cell line were in previous studies found to be comparable to those of fetal RPE cells. 10,11 The RPE cells were thawed and seeded directly on tissue-culture plates (Falcon; Becton Dickinson Labware, Bedford, MA, USA) at a density of 8000 cells/cm 2 .After reaching confluence, they were transferred at a split ratio of 1:5 and seeded onto new tissueculture plates and repeatedly transferred at a split ratio of 1:8 in order to increase the amount of cells of a given generation. When the cells had acquired a hexagonal shape and pigmentation after 2-4 weeks, they were applied in coculture experiments.…”

Section: Retinal Pigment Epithelial Cell Culturementioning

confidence: 99%

The Immune Privilege of the Eye: Human Retinal Pigment Epithelial Cells Selectively Modulate T-Cell ActivationIn Vitro

Kaestel

Lovato

Ødum

et al. 2005

Current Eye Research

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Section: Retinal Pigment Epithelial Cell Culturementioning

confidence: 99%

The Immune Privilege of the Eye: Human Retinal Pigment Epithelial Cells Selectively Modulate T-Cell ActivationIn Vitro

Kaestel

Lovato

Ødum

et al. 2005

Current Eye Research

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“…Studies with human RPE cell culture are prone to sampling errors as there is donor‐specific variation in the material. This variation can be at least partly eliminated by the use of cells from the ‘pseudoimmortalized’ RPE cell line ARPE19, which has been firmly accredited as a good RPE model (Jorgensen et al 2005; Kaestel et al 2001; Bailey et al 2004). Studies of ICG toxicity in ARPE19 cells have shown that prolonged exposure (30 mins−3 hours) causes decreased cell survival, even in doses as low as 0.5 mg/ml (Gale et al 2004; Ho et al 2003).…”

Section: Introductionmentioning

confidence: 99%

An isotonic preparation of 1 mg/ml indocyanine green is not toxic to hyperconfluent ARPE19 cells, even after prolonged exposure

Kiilgaard

Nissen

Cour

2006

Acta Ophthalmologica Scandinavica

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ABSTRACT.Purpose: To investigate the in vitro toxicity of indocyanine green and infracyanine green (ICG) to cultured ARPE19 cells, in particular with respect to the concentration and time dependence of this toxicity. Methods: ARPE19 cells were grown for at least 1 week past confluence (hyperconfluent cells) before being subjected to challenge with ICG. Cell survival was tested with the MTT assay.Results: When applied in isotonic solutions, ICG in all concentrations (below 5 mg/ml) and at all exposure times tested (2 mins-2 hours) was found not to affect the survival of ARPE19 cells. ARPE19 cultures older than 30 days were more resistant to a 5 mg/ml hypotonic ICG solution than younger cultures. Conclusion: When toxicity of ICG was tested in hyperconfluent ARPE19 cultures, these cells were found to be more resistant to the dye than has been previously reported for more immature ARPE19 cells.

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“…Our fvERM cells expressed low levels of the α -subunit-containing integrins, besides a reported expression of integrin α 4 in diabetic retinopathy [53, 55]. Interestingly, the presence of integrin β 2 subunit has been considered important factor in the RPE-T cell interaction [56]. …”

Section: Discussionmentioning

confidence: 85%

Functional and Molecular Characterization ofEx VivoCultured Epiretinal Membrane Cells from Human Proliferative Diabetic Retinopathy

Veréb

Lumi

Andjelić

et al. 2013

BioMed Research International

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Characterization of the cell surface marker phenotype of ex vivo cultured cells growing out of human fibrovascular epiretinal membranes (fvERMs) from proliferative diabetic retinopathy (PDR) can give insight into their function in immunity, angiogenesis, and retinal detachment. FvERMs from uneventful vitrectomies due to PDR were cultured adherently ex vivo. Surface marker analysis, release of immunity- and angiogenesis-pathway-related factors upon TNFα activation and measurement of the intracellular calcium dynamics upon mechano-stimulation using fluorescent dye Fura-2 were all performed. FvERMs formed proliferating cell monolayers when cultured ex vivo, which were negative for endothelial cell markers (CD31, VEGFR2), partially positive for hematopoietic- (CD34, CD47) and mesenchymal stem cell markers (CD73, CD90/Thy-1, and PDGFRβ), and negative for CD105. CD146/MCAM and CD166/ALCAM, previously unreported in cells from fvERMs, were also expressed. Secretion of 11 angiogenesis-related factors (DPPIV/CD26, EG-VEGF/PK1, ET-1, IGFBP-2 and 3, IL-8/CXCL8, MCP-1/CCL2, MMP-9, PTX3/TSG-14, Serpin E1/PAI-1, Serpin F1/PEDF, TIMP-1, and TSP-1) were detected upon TNFα activation of fvERM cells. Mechano-stimulation of these cells induced intracellular calcium propagation representing functional viability and role of these cells in tractional retinal detachment, thus serving as a model for studying tractional forces present in fvERMs in PDR ex vivo.

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Superantigen Presentation by Human Retinal Pigment Epithelial Cells to T Cells is Dependent on CD2–CD58 and CD18–CD54 Molecule Interactions

Cited by 11 publications

References 30 publications

The Immune Privilege of the Eye: Human Retinal Pigment Epithelial Cells Selectively Modulate T-Cell ActivationIn Vitro

The Immune Privilege of the Eye: Human Retinal Pigment Epithelial Cells Selectively Modulate T-Cell ActivationIn Vitro

An isotonic preparation of 1 mg/ml indocyanine green is not toxic to hyperconfluent ARPE19 cells, even after prolonged exposure

Functional and Molecular Characterization ofEx VivoCultured Epiretinal Membrane Cells from Human Proliferative Diabetic Retinopathy

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