Supercontinuum ultra wide range confocal microscope for reflectance spectroscopy of living matter and material science surfaces

Selci, S.; Bertani, Francesca Romana; Ferrari, L.

doi:10.1063/1.3631661

Cited by 13 publications

(13 citation statements)

References 32 publications

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“…The resulting excitation wavelength range of enables visualization of dozens of visible fluorophores, and all excitation peaks within a typical HiPco SWCNT sample [ 21 ]. This emergent laser technology has recently been employed for TIRF microscopy or for the study of nano-bio interactions [ 22 , 23 ].…”

Section: Methodsmentioning

confidence: 99%

Experimental Tools to Study Molecular Recognition within the Nanoparticle Corona

Landry

Kruss

Nelson

et al. 2014

Sensors

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Advancements in optical nanosensor development have enabled the design of sensors using syntheticmolecular recognition elements through a recently developed method called Corona Phase MolecularRecognition (CoPhMoRe). The synthetic sensors resulting from these design principles are highly selective for specific analytes, and demonstrate remarkable stability for use under a variety of conditions. An essential element of nanosensor development hinges on the ability to understand the interface between nanoparticles and the associated corona phase surrounding the nanosensor, an environment outside of the range of traditional characterization tools, such as NMR. This review discusses the need for new strategies and instrumentation to study the nanoparticle corona, operating in both in vitro and in vivo environments. Approaches to instrumentation must have the capacity to concurrently monitor nanosensor operation and the molecular changes in the corona phase. A detailed overview of new tools for the understanding of CoPhMoRe mechanisms is provided for future applications.

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Section: Methodsmentioning

confidence: 99%

Experimental Tools to Study Molecular Recognition within the Nanoparticle Corona

Landry

Kruss

Nelson

et al. 2014

Sensors

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“…The theoretical lines are computed by a full multilayer calculation [18] based on the Abelés matrix method [19]. Dielectric functions for silicon and silicon oxide are taken from Palik [26].…”

Section: Resultsmentioning

confidence: 99%

Living Matter Observations with a Novel Hyperspectral Supercontinuum Confocal Microscope for VIS to Near-IR Reflectance Spectroscopy

Bertani

Ferrari

Mussi

et al. 2013

Sensors

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A broad range hyper-spectroscopic microscope fed by a supercontinuum laser source and equipped with an almost achromatic optical layout is illustrated with detailed explanations of the design, implementation and data. The real novelty of this instrument, a confocal spectroscopic microscope capable of recording high resolution reflectance data in the VIS-IR spectral range from about 500 nm to 2.5 μm wavelengths, is the possibility of acquiring spectral data at every physical point as defined by lateral coordinates, X and Y, as well as at a depth coordinate, Z, as obtained by the confocal optical sectioning advantage. With this apparatus we collect each single scanning point as a whole spectrum by combining two linear spectral detector arrays, one CCD for the visible range, and one InGaAs infrared array, simultaneously available at the sensor output channel of the home made instrument. This microscope has been developed for biomedical analysis of human skin and other similar applications. Results are shown illustrating the technical performances of the instrument and the capability in extracting information about the composition and the structure of different parts or compartments in biological samples as well as in solid statematter. A complete spectroscopic fingerprinting of samples at microscopic level is shown possible by using statistical analysis on raw data or analytical reflectance models based on Abelés matrix transfer methods.

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“…This explicit purpose has been partially or implicitly already demonstrated in the previously presented papers. In fact, in the first paper where the HSCM results have been shown [1], the layer models have been used for a numerical best fit of the measured reflectivity of golden structures of a technological test sample (a film made up of 60 nm Au/10 nm Ti over a Si wafer substrate), with good success.…”

Section: Discussionmentioning

confidence: 99%

“…The wavelengths' stack originates from the merge of two different detectors [1] with the IR part, the InGaAs array detector, made by a fixed set of 256 wavelengths, while the remaining part is coming from the CCD (Charge-Coupled Device) detector channels, reduced by a factor of 2 by hardware binning. The exit pinhole has a diameter of 100 µm.…”

Section: Rbc Datamentioning

confidence: 99%

“…The instrument set-up and experimental details have been already presented [1] and discussed [2]. The main application of this HSCM, working in a broad VIS and NIR spectral range, has been within biology, investigating for instance many types of cells, like the acquisition of multi-dimensional spectral images of human melanoma cells [2], or the discrimination of HaCaT and melanoma cells in a co-culture model, for which reflectance data have shown good matching between spectral signature and cells' nature or evolution [3].…”

Section: Introductionmentioning

confidence: 99%

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Phase and Index of Refraction Imaging by Hyperspectral Reflectance Confocal Microscopy

Selci

2016

Molecules

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A hyperspectral reflectance confocal microscope (HSCM) was realized by CNR-ISC (Consiglio Nazionale delle Ricerche-Istituto dei Sistemi Complessi) a few years ago. The instrument and data have been already presented and discussed. The main activity of this HSCM has been within biology, and reflectance data have shown good matching between spectral signatures and the nature or evolution on many types of cells. Such a relationship has been demonstrated mainly with statistical tools like Principal Component Analysis (PCA), or similar concepts, which represent a very common approach for hyperspectral imaging. However, the point is that reflectance data contains much more useful information and, moreover, there is an obvious interest to go from reflectance, bound to the single experiment, to reflectivity, or other physical quantities, related to the sample alone. To accomplish this aim, we can follow well-established analyses and methods used in reflectance spectroscopy. Therefore, we show methods of calculations for index of refraction n, extinction coefficient k and local thicknesses of frequency starting from phase images by fast Kramers-Kronig (KK) algorithms and the Abeles matrix formalism. Details, limitations and problems of the presented calculations as well as alternative procedures are given for an example of HSCM images of red blood cells (RBC).

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Supercontinuum ultra wide range confocal microscope for reflectance spectroscopy of living matter and material science surfaces

Cited by 13 publications

References 32 publications

Experimental Tools to Study Molecular Recognition within the Nanoparticle Corona

Experimental Tools to Study Molecular Recognition within the Nanoparticle Corona

Living Matter Observations with a Novel Hyperspectral Supercontinuum Confocal Microscope for VIS to Near-IR Reflectance Spectroscopy

Phase and Index of Refraction Imaging by Hyperspectral Reflectance Confocal Microscopy

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