Supercritical carbon dioxide extracted extracellular matrix material from adipose tissue

Wang, Jun Kit; Luo, Baiwen; Guneta, Vipra; Li, Liang; Foo, Selin; Dai, Yumei; Tan, Timothy Thatt Yang; Tan, Nguan Soon; Choong, Cleo; Wong, Marcus

doi:10.1016/j.msec.2017.02.002

Cited by 56 publications

(70 citation statements)

References 51 publications

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“…ADM possess biocompatible and biodegradable properties; therefore, it can be used as a form of wound dressing for wound management [20]. ADM is mainly composed of extracellular matrix (ECM), which comprises a wide range of biological factors that induce cell growth and regulate tissue responses, and the 3D microstructure of ECM in ADM serves as scaffolds for tissue engineering [21][22][23]. The 3D structure offers tensile attachment positions for specific cells, cell surface receptors, or signaling factors that modulate the wound healing process [7,24].…”

Section: Introductionmentioning

confidence: 99%

“…However, the existing decellularization methods used for ADM preparation are time consuming and involve chemicals that damage the components of ECM [26]. Supercritical carbon dioxide (scCO2) extraction technology has proved to be an excellent alternative method because it is environmentally friendly, nontoxic, and efficient in eliminating toxic chemicals and retaining ECM components [22]. Decellularization technology using scCO2 is advantageous, with no solvent residue, no off-odors, and complete solubilization and removal of hydrocarbons, including lipids.…”

Section: Introductionmentioning

confidence: 99%

“…Enhanced cell proliferation, growth, and differentiation were observed in scCO 2 -treated ADM with ASCs; thus, scCO 2 -treated ADM might be a key component of tissue engineering [22]. Delivery of ASCs through ADM scaffolds accelerated diabetic wound healing through a paracrine mechanism, which enhanced granulation tissue formation and increased re-epithelialization and neovascularization [29].…”

Section: Introductionmentioning

confidence: 99%

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Supercritical Carbon Dioxide-decellularized Porcine Acellular Dermal Matrix combined with Autologous Adipose-derived Stem Cells: Its Role in Accelerated Diabetic Wound Healing

Chou¹,

Lin²,

Wu³

et al. 2020

Int. J. Med. Sci.

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Diabetes mellitus (DM) causes impaired wound healing by affecting one or more of the biological mechanisms of hemostasis, inflammation, proliferation, and remodeling and a large number of cell types, extracellular components, growth factors, and cytokines. Interventions targeted toward these mechanisms might accelerate the wound healing process. To evaluate the wound healing efficacy of supercritical carbon dioxide (scCO2)-decellularized porcine acellular dermal matrix (ADM) combined with autologous adipose-derived stem cells (ASCs) in streptozotocin (STZ)-induced DM rats. DM was induced by injecting rats with STZ; dorsal full-thickness skin (5 × 5 cm 2) was created and treated with and without ASCs-scCO2-treated ADM to evaluate the wound healing rate through histological examination, fluorescence microscopic observation, and immunohistochemical analysis. In the present study, complete decellularization of the porcine dermal matrix was achieved through scCO2. Isolation of ASCs was conducted and evaluated using CD29 + /CD31 − /CD45 − /CD90 + markers in flow cytometry, which indicated that more than 90% of cells were ASCs. The percentage of cells labeled with CD29 + and CD90 + was found to be 97.50% and 99.69%, respectively. The wound healing rate increased in all groups relative to the group with the DM wound without treatment. DM wound treated with ADM-ASCs showed significantly higher (p < 0.01) wound healing rate than DM wound without treatment. ADM-ASC-treated rats showed significantly increased epidermal growth factor, Ki67, and prolyl 4-hydroxylase and significantly decreased CD45 compared with the group with the DM wound without treatment. The intervention comprising ADM decellularized from porcine skin by using scCO2 and ASCs was proven to improve diabetic wound healing. ADM-ASCs had a positive effect on epidermal regeneration, anti-inflammation, collagen production and processing, and cell proliferation; thus, it accelerated wound healing.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Supercritical Carbon Dioxide-decellularized Porcine Acellular Dermal Matrix combined with Autologous Adipose-derived Stem Cells: Its Role in Accelerated Diabetic Wound Healing

Chou¹,

Lin²,

Wu³

et al. 2020

Int. J. Med. Sci.

View full text Add to dashboard Cite

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“…Pressurized carbon dioxide technology has been proposed as a nontoxic, non-residual and suitable one-step alternative to physical-chemical protocols for tissue decellularization [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…Some authors have reported promising decellularization rates by pressurized CO2 with or without cosolvents for heart valves, arteries, adipose tissue and bones [10][11][12]19,20]; however, no studies have considered retina, which is a very mechanically sensitive tissue. Previous studies using pressurized CO2 were usually performed at ~37 °C and up to 300 bar using ethanol as a cosolvent and found the absence of nuclei after treatment (denuclearization) to be the main effect.…”

Section: Introductionmentioning

confidence: 99%

Pressurized carbon dioxide combined with aqueous ethanol as cosolvent induces efficient delipidation of porcine retina for their use as bioscaffolds

Gil-Ramírez

Spangenberg

Sartipy

et al. 2019

Journal of CO2 Utilization

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Retina damage results in both health and social problems. To treat retina damage, extracellular matrix technology has gained interest in the scientist community. Bioscaffolds production is based in the use of a `cleaned` tissue, free of undesired cellular components, for further recellularization using healthy cells. Removal of lipids is an important step to improve both deand recellularization. This work shows, for the first time, the efficiency of pressurized carbon dioxide (CO2) fluids for the delipidation of porcine retina tissue. Pressurized CO2-EtOH-H2O (0.87 XCO2) at 300 bar and 37 °C for 1h completely removed the 28 lipid species found in untreated retina (classified as phosphatidylcholines, phosphatidylethanolamines and sphingomyelins). When the ethanolic mixture was replaced by limonene at same conditions, lipid content decreased by more than a 50%, indicating reasonably good solubility of retina lipids in pressurized CO2limonene; however, no delipidation was observed using neat supercritical CO2. In conclusion, pressurize CO2 technologies can be applied for delipidation of retina. Whether the resulting bioscaffold can be recellularized and used in transplantation remain to be shown.

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Hydrophilic Components as Key Active Ingredients in Adipose‐Derived Matrix Bioscaffolds for Inducing Fat Regeneration

Ma,

Yue,

Wang

et al. 2024

Adv Healthcare Materials

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Decellularized adipose‐derived matrix (DAM) has emerged as a promising biomaterial for soft tissue reconstruction. However, due to a lack of research on its complex composition, the understanding of the key components in DAM remains limited, leading to inconsistent adipogenic properties and challenges in optimizing preparation methods purposefully. In this study, it is proposed for the first time that DAM comprises two distinct components: hydrophilic (H‐DAM) and lipophilic (L‐DAM), each with markedly different effects on fat regeneration. It is confirmed that H‐DAM is the key component for inducing fat regeneration due to its enhanced cell–cell and cell–scaffold interactions, primarily mediated by the Hedgehog signaling pathway. In contrast, L‐DAM exhibits poor cell adhesion and contains more antigenic components, leading to a higher immunoinflammatory response and reduced adipogenesis. In addition, it is found that intracellular proteins, which are more abundant in H‐DAM, can be retained as beneficial components due to their hydrophilicity, contrary to the conventional view that they shall be removed. Accordingly, a purified bioscaffold with unprecedented efficacy is proposed for fat regeneration and reduced immunogenicity. This finding provides insights for developing scaffolds for fat regeneration and promotes the realization of xenotransplantation.

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Supercritical carbon dioxide extracted extracellular matrix material from adipose tissue

Cited by 56 publications

References 51 publications

Supercritical Carbon Dioxide-decellularized Porcine Acellular Dermal Matrix combined with Autologous Adipose-derived Stem Cells: Its Role in Accelerated Diabetic Wound Healing

Supercritical Carbon Dioxide-decellularized Porcine Acellular Dermal Matrix combined with Autologous Adipose-derived Stem Cells: Its Role in Accelerated Diabetic Wound Healing

Pressurized carbon dioxide combined with aqueous ethanol as cosolvent induces efficient delipidation of porcine retina for their use as bioscaffolds

Hydrophilic Components as Key Active Ingredients in Adipose‐Derived Matrix Bioscaffolds for Inducing Fat Regeneration

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