Supercritical CO2 Extract from Microalga Tetradesmus obliquus: The Effect of High-Pressure Pre-Treatment

Vladić, Jelena; Jerković, Igor; Radman, Sanja; Jazić, Jelena Molnar; Ferreira, Alice; Maletić, Snežana; Gouveia, Luísa

doi:10.3390/molecules27123883

Cited by 3 publications

(3 citation statements)

References 55 publications

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“…Loliolide, the only monoterpernoid lactone detected in our study, was previously detected in C. spongiosus f. verticillatus by El Hattab et al [57]. It has also been detected in the genus Ericaria [28], D. vermicularis [40], and the microalga Tetradesmus obliquus [63]. Silva et al [64] demonstrated the neuroprotective, anti-inflammatory, and antioxidant effects of loliolide in an extract of Codium tomentosum.…”

Section: Non-target Screening Of Non-volatile Compounds In Ethanol Ex...supporting

confidence: 76%

Seasonal Monitoring of Volatiles and Antioxidant Activity of Brown Alga Cladostephus spongiosus

et al. 2023

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Cladostephus spongiosus was harvested once a month during its growing season (from May to August) from the Adriatic Sea. Algal volatile organic compounds (VOCs) were obtained by headspace solid-phase microextraction (HS-SPME) and hydrodistillation (HD) and analysed by gas chromatography and mass spectrometry (GC-MS). The effects of air drying and growing season on VOCs were determined. Two different extraction methods (ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE)) were used to obtain ethanolic extracts of C. spongiosus. In addition, the seasonal antioxidant potential of the extracts was determined, and non-volatile compounds were identified from the most potent antioxidant extract. Aliphatic compounds (e.g., pentadecane) were predominantly found by HS-SPME/GC-MS. Hydrocarbons were more than twice as abundant in the dry samples (except in May). Aliphatic alcohols (e.g., hexan-1-ol, octan-1-ol, and oct-1-en-3-ol) were present in high percentages and were more abundant in the fresh samples. Hexanal, heptanal, nonanal, and tridecanal were also found. Aliphatic ketones (octan-3-one, 6-methylhept-5-en-2-one, and (E,Z)-octa-3,5-dien-2-one) were more abundant in the fresh samples. Benzene derivatives (e.g., benzyl alcohol and benzaldehyde) were dominant in the fresh samples from May and August. (E)-Verbenol and p-cymen-8-ol were the most abundant in dry samples in May. HD revealed aliphatic compounds (e.g., heptadecane, pentadecanal, (E)-heptadec-8-ene, (Z)-heptadec-3-ene), sesquiterpenes (germacrene D, epi-bicyclosesquiphellandrene, gleenol), diterpenes (phytol, pachydictyol A, (E)-geranyl geraniol, cembra-4,7,11,15-tetraen-3-ol), and others. Among them, terpenes were the most abundant (except for July). Seasonal variations in the antioxidant activity of the ethanolic extracts were evaluated via different assays. MAE extracts showed higher peroxyl radical inhibition activity from 55.1 to 74.2 µM TE (Trolox equivalents). The highest reducing activity (293.8 µM TE) was observed for the May sample. Therefore, the May MAE extract was analysed via high-performance liquid chromatography with high-resolution mass spectrometry and electrospray ionisation (UHPLC-ESI-HRMS). In total, 17 fatty acid derivatives, 9 pigments and derivatives, and 2 steroid derivatives were found. The highest content of pheophorbide a and fucoxanthin, as well as the presence of other pigment derivatives, could be related to the observed antioxidant activity.

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Section: Non-target Screening Of Non-volatile Compounds In Ethanol Ex...supporting

confidence: 76%

Seasonal Monitoring of Volatiles and Antioxidant Activity of Brown Alga Cladostephus spongiosus

et al. 2023

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“…17, 21, 24) and two diacylglycerols (no. [32][33] showed an increase in the N-deprived samples. A long-chain fatty acid C:20 (no.…”

Section: Non-target Screening Of Non-volatile Compounds In Ethanol Ex...mentioning

confidence: 89%

“…Menzel et al [31] used it as a biomarker in haptophytes, diatoms, dinoflagellates, and eustigmatophytes while investigating the deposition of sapropel. Loliolide was detected in acetone and MeOH extracts of the Antarctic diatoms Craspedostauros ineffabilis and C. zucchelli, as well as in a supercritical CO 2 extract of the green microalga Tetradesmus obliquus [32]. In the Ndeprived sample, it was 1.2 times more abundant than in the control sample, 1.8 times more than in the P-deprived sample and 5.0 times more than in the Si-deprived sample.…”

Section: Non-target Screening Of Non-volatile Compounds In Ethanol Ex...mentioning

confidence: 95%

Influence of Nutrient Deprivation on the Antioxidant Capacity and Chemical Profile of Two Diatoms from Genus Chaetoceros

Frleta Matas,

Radman,

Čagalj

et al. 2024

Marine Drugs

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The limited availability of phosphate, nitrogen and silicon in the growth media affects the growth, cellular processes, and metabolism of diatoms. Silicon deficiency primarily affects diatom morphology, while phosphate deficiency reduces the production of nucleic acids and phospholipids. Differences in pigment and protein composition are mainly due to nitrogen deficiency. In this study, Chaetoceros socialis and Chaetoceros costatus were cultured under phosphate, nitrogen, and silicon deprivation conditions. The diatom biomass was collected during the stationary growth phase and extracted with 70% ethanol under ultrasonication. The chemical profiles of the extracts were analyzed by high-performance liquid chromatography with high-resolution mass spectrometry with electrospray ionisation (UHPLC-ESI-HRMS), while the antioxidant capacity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and oxygen radical absorbance capacity (ORAC) assays. Pigments, fatty acids, sterols, and derivatives were detected in both species. The total phenolic content in the extracts ranged from 46.25 ± 1.08 to 89.38 ± 6.21 mg of gallic acid equivalent (GAE)/L and from 29.58 ± 1.08 to 54.17 ± 1.18 mg GAE/L. for C. costatus and C. socialis, respectively. Antioxidant activity was higher in C. costatus extracts, especially those obtained from nitrogen-deprived media. The results of this study contribute to the existing knowledge and the ongoing efforts to overcome application and commercialization barriers of microalgae for wide-ranging potential in different industries.

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Exploring the potential of deep eutectic systems for the preservation of the chemical profile and antibacterial potential of dill (Anethum graveolens L.) supercritical CO₂ extracts

Vladić,

Jerković,

Pavić

et al. 2025

The Journal of Supercritical Fluids

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Supercritical CO2 Extract from Microalga Tetradesmus obliquus: The Effect of High-Pressure Pre-Treatment

Cited by 3 publications

References 55 publications

Seasonal Monitoring of Volatiles and Antioxidant Activity of Brown Alga Cladostephus spongiosus

Seasonal Monitoring of Volatiles and Antioxidant Activity of Brown Alga Cladostephus spongiosus

Influence of Nutrient Deprivation on the Antioxidant Capacity and Chemical Profile of Two Diatoms from Genus Chaetoceros

Exploring the potential of deep eutectic systems for the preservation of the chemical profile and antibacterial potential of dill (Anethum graveolens L.) supercritical CO₂ extracts

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