Supercritical Fluid Chromatography and Extraction

Chester, T. L.; Pinkston, J. David; Raynie, Douglas E.

doi:10.1021/a1960017i

Cited by 107 publications

(48 citation statements)

References 513 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Extracts of different compositions can be obtained by different extraction methods applied to natural products (20)(21)(22)(23)(24). The GC-MS profile of the ethyl acetate extract is shown in Figure 2.…”

Section: Gas Chromatography-mass Spectrometrymentioning

confidence: 99%

Cytotoxicity of Marchantia convoluta leaf extracts to human liver and lung cancer cells

Xiao

Chen

Zhang

et al. 2006

Braz J Med Biol Res

View full text Add to dashboard Cite

The cytotoxicity of three extracts (petroleum ether, ethyl acetate and n-butanol) from a plant used in folk medicine, Marchantia convoluta, to human non-small cell lung carcinoma (H1299) and liver carcinoma (HepG2) cell lines was tested. After 72-h incubation of lung and liver cancer cell cultures with varying concentrations of extracts (15 to 200 µg/mL), cytotoxicity was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and reported in terms of cell viability. The extracts that showed a significant cytotoxicity were subjected to gas chromatography-mass spectrometry analysis to identify the components. The ethyl acetate, but not the petroleum ether or n-butanol extract, had a significant cytotoxicity against lung and liver carcinoma cells with IC 50 values of 100 and 30 µg/mL, respectively. A high concentration of ethyl acetate extract (100 µg/mL) rapidly reduced the number of H1299 cells. At lower concentrations of ethyl acetate extract (15, 30, and 40 µg/mL), the numbers of HepG2 cells started to decrease markedly. Gas chromatography-mass spectrometry analysis of the ethyl acetate extract revealed the presence of several compounds such as phytol (23.42%), 1,2,4-tripropylbenzene (13.09%), 9-cedranone (12.75%), ledene oxide (7.22%), caryophyllene (1.82%), and caryophyllene oxide (1.15%). HPLC analysis result showed that there were no flavonoids in ethyl acetate extract, but flavonoids are abundant in n-butanol extract. Further studies are needed regarding the identification, toxicity, and mechanism of action of active compounds. Correspondence

show abstract

Section: Gas Chromatography-mass Spectrometrymentioning

confidence: 99%

Cytotoxicity of Marchantia convoluta leaf extracts to human liver and lung cancer cells

Xiao

Chen

Zhang

et al. 2006

Braz J Med Biol Res

View full text Add to dashboard Cite

show abstract

“…The liquid products in alcohol can be used directly as liquid fuel because alcohol itself is a good fuel [3]. Supercritical fluids are regarded as promising solvents for industrial use in the coming decades and their special physical properties have caught worldwide attention for a long period of time [1,4]. Several reviews have highlighted developments in theory, application, and instrumentation [5,6].…”

Section: Introductionmentioning

confidence: 99%

Degradation Characteristics of Wood Using Supercritical Alcohols

Poudel

2012

Energies

View full text Add to dashboard Cite

Abstract:In this work, the characteristics of wood degradation using supercritical alcohols have been studied. Supercritical ethanol and supercritical methanol were used as solvents. The kinetics of wood degradation were analyzed using the nonisothermal weight loss technique with heating rates of 3.1, 9.8, and 14.5 °C/min for ethanol and 5.2, 11.3, and 16.3 °C/min for methanol. Three different kinetic analysis methods were implemented to obtain the apparent activation energy and the overall reaction order for wood degradation using supercritical alcohols. These were used to compare with previous data for supercritical methanol. From this work, the activation energies of wood degradation in supercritical ethanol were obtained as 78.0-86.0, 40.1-48.1, and 114 kJ/mol for the different kinetic analysis methods used in this work. The activation energies of wood degradation in supercritical ethanol were obtained as 78.0-86.0, 40.1-48.1, and 114 kJ/mol. This paper also includes the analysis of the liquid products obtained from this work. The characteristic analysis of liquid products on increasing reaction temperature and time has been performed by GC-MS. The liquid products were categorized according to carbon numbers and aromatic/aliphatic components. It was found that higher conversion in supercritical ethanol occurs at a lower temperature than that of supercritical methanol. The product analysis shows that the majority of products fall in the 2 to 15 carbon number range.

show abstract

“…Pesticides in matrices similar to CHM have been separated using various techniques, for instance solvent extraction and column adsorption. The general drawbacks, such as the use of large amounts of solvents, time-consuming and labor-intensive processes, and considerable production of waste, associated with these classical extraction techniques have been reduced by using matrix solid-phase dispersion and supercritical fluid extract (SFE) (Chester et al, 1998). SFE has been shown to be an efficient and rapid method for the isolation of pesticides from fruit, vegetables and medicinal plant matrices (Barnabas et al, 1994;Li et al, 1996;Qiu et al, 1997;Chen et al, 2000;Nerín et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Supercritical fluid extraction for the separation of organochlorine pesticides residue in Angelica sinensis

Zhao

Hao

Huan-xin

et al. 2002

Biomedical Chromatography

View full text Add to dashboard Cite

A method involving the simultaneous extraction and separation of 12 organochlorine pesticides (OCPs) from Angelicae sinensis was developed using supercritical fluid extraction (SFE). The pesticides in the study were alpha-, beta-, gamma- and delta-benzene hexachloride, PCNB (pentachloro- nitrobenzene), PCA (pentachloroaniline), HEPT (heptachlor), MPCPS (methyl-pentachlorophenyl sulfide), pp'-DDE [1,1-dichloro-2,2-bis (p-chlorophenyl) ethylene], op'-DDT [1,1,1,-trichloro-2-(o-chlorophenyl)-2-(p-chlorophenyl) ethane], pp'-DDD [1,1-dichloro-2,2-bis(p-chlorophenyl) ethane], and pp'-DDT [1,1,1,-trichloro-2,2-bis (p-chlorophenyl)ethane]. The extraction conditions were optimized as follows: pure CO(2), extraction pressure 15 MPa, extraction temperature 60 degrees C, extraction time 20 min, and flow-rate 1.5 mL/min. A GC method with electron capture detection was employed to determine the OCPs in Angelicae sinensis. An HPLC method was developed for the quantitative determination of active constituents. The SFE provided high decontamination rate of OCPs and low loss of active constituents in Angelicae sinensis.

show abstract

Supercritical Fluid Chromatography and Extraction

Cited by 107 publications

References 513 publications

Cytotoxicity of Marchantia convoluta leaf extracts to human liver and lung cancer cells

Cytotoxicity of Marchantia convoluta leaf extracts to human liver and lung cancer cells

Degradation Characteristics of Wood Using Supercritical Alcohols

Supercritical fluid extraction for the separation of organochlorine pesticides residue in Angelica sinensis

Contact Info

Product

Resources

About