2015
DOI: 10.1055/s-0035-1557823
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Supercritical Fluid Chromatography as an Alternative Tool for the Qualitative and Quantitative Analysis of Metarhizium brunneum Metabolites from Culture Broth

Abstract: A fast and selective ultrahigh-performance supercritical fluid chromatography photodiode array detector method was established for the qualitative and quantitative analysis of destruxins, cyclic hexadepsipeptides, from fungal culture broth samples. Prior to analysis, sample purification was carried out using an off-line solid-phase extraction protocol on a reversed-phase material in order to remove unwanted matrix constituents. For separation, detection, and identification, an ultrahigh-performance supercritic… Show more

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Cited by 5 publications
(2 citation statements)
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“…Due to concerns that this use entails risks to humans and the environment, the development of validated analysis methods is of great interest. Optimum resolution was obtained on an Acquity UPC² BEH 2-EP column with a mixture of supercritical CO 2 and methanol/acetonitrile (8 : 2 v/v) containing 0.02 % trifluoroacetic acid as the mobile phase [63]. As shown in ▶ Fig.…”
Section: Miscellaneousmentioning
confidence: 99%
See 1 more Smart Citation
“…Due to concerns that this use entails risks to humans and the environment, the development of validated analysis methods is of great interest. Optimum resolution was obtained on an Acquity UPC² BEH 2-EP column with a mixture of supercritical CO 2 and methanol/acetonitrile (8 : 2 v/v) containing 0.02 % trifluoroacetic acid as the mobile phase [63]. As shown in ▶ Fig.…”
Section: Miscellaneousmentioning
confidence: 99%
“…Representative UHPSFC-PDA chromatogram of a Metarhizium brunneum DCM crude extract and a destruxine standard mixture containing dtx A, B, D, E, and E-diol. Reproduced with permission from[63].…”
mentioning
confidence: 99%