Supercritical fluid chromatography with small particle diameter packed columns

Gere, Dennis R.; Board, Robert D.; McManigill, Douglass.

doi:10.1021/ac00241a032

Cited by 211 publications

(52 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…pSFC was employed in very early research work, then it became less popular because of the marketing strategy of cSFC, but in the end it regained its popularity when packed columns were found to have a wider application range than open tubular capillary columns. The first commercial pSFC was introduced by Hewlett Packard in 1981 as a modification of an HPLC system with independent flow, composition, pressure, and temperature controls (Gere et al, 1982). Unlike cSFC, pSFC enabled researchers to add more organic modifier to the supercritical fluid, and thereby to increase its polarity and widen the range of analyzable compounds (Crowther and 1.…”

Section: History Of Supercritical Fluid Chromatographymentioning

confidence: 99%

Theory and Practice of UHPLC and UHPLC–MS

Guillarme

Veuthey

2017

Handbook of Advanced Chromatography /Mass Spectrometry Techniques

View full text Add to dashboard Cite

Section: History Of Supercritical Fluid Chromatographymentioning

confidence: 99%

Theory and Practice of UHPLC and UHPLC–MS

Guillarme

Veuthey

2017

Handbook of Advanced Chromatography /Mass Spectrometry Techniques

View full text Add to dashboard Cite

“…Only a few modifications of the GC hardware were required to work properly with supercritical fluid. Surprisingly, the first commercial SFC instrument marketed by Hewlett Packard in 1983, based on previous studies of Gere [230], was much more inspired by LC set-ups. This system was designed for the use of packed column to perform separation under supercritical conditions, and offered the possibility to modify the pure supercritical mobile phase with cosolvent thanks to an innovative binary pump [231].…”

Section: Description Of Sfcmentioning

confidence: 99%

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

Fekete¹,

Perrenoud²,

Guillarme

2014

CCHG

View full text Add to dashboard Cite

Abstract:The current trend in high performance liquid chromatography (HPLC) tends toward the achievement of higher separation efficiency and shorter analysis time. Indeed, better performance in LC has become increasingly important in recent years mainly driven by the challenges of either analyzing more complex samples or increasing the numbers of samples per time unit. In the recent development of particle technology, the use of fully porous sub-2 m particles and sub-3 m shell particles have received considerable attention. Beside packed columns, the new generation of silica-based monolithic columns also offers very high separation power. However, to take full advantage of these innovative phases, the chromatographic system has also to be drastically optimized in terms of upper pressure limit and system volume.This revolution in column technology now spreads and covers several modes of liquid chromatography such as reversedphase liquid chromatography (RPLC), hydrophilic interaction liquid chromatography (HILIC), or even supercritical fluid chromatography (SFC). The HILIC and SFC, which can be considered as alternative modes of chromatography, could also be useful to extend the applicability of chromatography towards the analysis of very hydrophilic and lipophilic compounds, respectively.The present review gives an insight about the theory behind the success of current column technology and presents a summary of latest applications, using various modes of one-dimensional chromatography (RPLC, HILIC, SFC). This paper also shows that theoretically expected column efficiency could sometimes be compromised in practical work especially in the case of narrow bore columns.

show abstract

“…Due to the low viscosity of near critical mobile phases, flow rate in SFC can be high, and number of theoretical stages remains nearly constant over a wide range of flow rate. A more detailed discussion of chromatographic fundamentals and especially analytical applications of SFC can be found in the abundant literature on analytical SFC (Gere et al, 1982;Lee and Markides, 1990;Smith, 1988;Wenclawiak, 1992;White, 1988). …”

Section: Gas Chromatography Apparatusmentioning

confidence: 99%