Supercritical fluid extraction and chromatography of artemisinin and artemisinic acid. An improved method for the analysis ofArtemisia annua samples

Köhler, Marcel; Haerdi, W.; Christen, Philippe; Veuthey, Jean‐Luc

doi:10.1002/(sici)1099-1565(199709/10)8:5<223::aid-pca370>3.0.co;2-a

Cited by 34 publications

(27 citation statements)

References 22 publications

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“…Several sesquiterpene lactones, such as santonin [7], parthenolide [8], costunolide [9] and artemisinin [10,11] were already extracted by SFE with good results.…”

Section: Introductionmentioning

confidence: 99%

Supercritical carbon dioxide extraction of tagitinin C from Tithonia diversifolia

Ziémons

Goffin

Lejeune

et al. 2005

The Journal of Supercritical Fluids

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Different parameters as temperature, pressure, solvent mass and sample granulometry governing the extraction yield of tagitinin C from the aerial parts of Tithonia diversifolia were optimised.An experimental design was carried out to map the effects of pressure (at 20.3, 30.4 and 40.5 MPa) and temperature (at 40, 60 and 80• C) on the extraction yield of the active component and to determine the optimal conditions for the extraction of tagitinin C from T. diversifolia. The best conditions are met for a pressure of 35.0 MPa and a temperature of 68• C. The effect of the particle size was studied under low pressure (13.7 MPa) and temperature (40 • C) conditions, which failed to extract quantitatively the tagitinin C from leaves sieved to 250 m size. The reduction of the particle size increased the extraction yield which became comparable to that of the optimised SFE for the particle in the range of 0-63 m.From the analysis of extraction kinetic curves of 200 mg of plant with supercritical carbon dioxide (range of 5-30 g), it appears that 15 g of this supercritical fluid is never limiting.The optimised supercritical fluid extraction (SFE) was compared favourably to Soxhlet extraction with dichloromethane (S) and to maceration followed by lixiviation with diethyl ether (ML), which gave similar extraction yields but higher extract content of tagitinin C were found using SFE (15.6 and 30.7% w/w tagitinin C in S and ML extracts, respectively, versus 52.8% in SFE extract).

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“…Several sesquiterpene lactones, such as santonin [7], parthenolide [8], costunolide [9] and artemisinin [10,11] were already extracted by SFE with good results.…”

Section: Introductionmentioning

confidence: 99%

Supercritical carbon dioxide extraction of tagitinin C from Tithonia diversifolia

Ziémons

Goffin

Lejeune

et al. 2005

The Journal of Supercritical Fluids

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“…The most common detector in pSFC is obviously the UV spectrophotometer [255], but the latter must be equipped with a special cell able to withstand the backpressure from the restrictor located downstream of the detector. The use of evaporative light scattering detector (ELSD) has also often been described in the literature [256][257][258]. Finally, it is also possible to couple pSFC with MS and this hyphenation was already described over 20 years ago [259].…”

Section: Advantages and Limitations Of Sfcmentioning

confidence: 67%

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

Fekete¹,

Perrenoud²,

Guillarme

2014

CCHG

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Abstract:The current trend in high performance liquid chromatography (HPLC) tends toward the achievement of higher separation efficiency and shorter analysis time. Indeed, better performance in LC has become increasingly important in recent years mainly driven by the challenges of either analyzing more complex samples or increasing the numbers of samples per time unit. In the recent development of particle technology, the use of fully porous sub-2 m particles and sub-3 m shell particles have received considerable attention. Beside packed columns, the new generation of silica-based monolithic columns also offers very high separation power. However, to take full advantage of these innovative phases, the chromatographic system has also to be drastically optimized in terms of upper pressure limit and system volume.This revolution in column technology now spreads and covers several modes of liquid chromatography such as reversedphase liquid chromatography (RPLC), hydrophilic interaction liquid chromatography (HILIC), or even supercritical fluid chromatography (SFC). The HILIC and SFC, which can be considered as alternative modes of chromatography, could also be useful to extend the applicability of chromatography towards the analysis of very hydrophilic and lipophilic compounds, respectively.The present review gives an insight about the theory behind the success of current column technology and presents a summary of latest applications, using various modes of one-dimensional chromatography (RPLC, HILIC, SFC). This paper also shows that theoretically expected column efficiency could sometimes be compromised in practical work especially in the case of narrow bore columns.

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“…The use of scCO 2 as a solvent is well known and practiced commercially not only for natural products extraction [33] but also as a reaction solvent [34]. Therefore, methanol or ethanol is used as modifier to increase the solubility and establish a viable process [20,37,39,40]. Thermodynamic data on solubility of artemisinin in scCO 2 was recently remeasured and summarized [38].…”

Section: Alternative Solvents For Extraction Of Artemisininmentioning

confidence: 99%

Green Extraction of Artemisinin from Artemisia annua L

Lapkin

2015

Green Extraction of Natural Products

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Supercritical fluid extraction and chromatography of artemisinin and artemisinic acid. An improved method for the analysis ofArtemisia annua samples

Cited by 34 publications

References 22 publications

Supercritical carbon dioxide extraction of tagitinin C from Tithonia diversifolia

Supercritical carbon dioxide extraction of tagitinin C from Tithonia diversifolia

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

Green Extraction of Artemisinin from Artemisia annua L

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