Supercritical Fluid Extraction and Ultra Performance Liquid Chromatography of Respiratory Quinones for Microbial Community Analysis in Environmental and Biological Samples

Hanif, M.; Atsuta, Yoichi; Fujie, Koichi; Daimon, Hiroyuki

doi:10.3390/molecules17032628

Cited by 12 publications

(10 citation statements)

References 34 publications

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“…Pada saat ini, kemajuan metodologi untuk menganalisis komunitas mikroba dari berbagai jenis sampel telah memungkinkan kita untuk mengetahui secara lebih detail komposisi dan fungsi komunitas mikroba tersebut. Namun, pengembangan dan penerapan metode yang lebih handal untuk memperoleh pemahaman secara komprehensif tentang struktur komunitas mikrobia masih merupakan tantangan [4].…”

Section: Pendahuluanunclassified

Aplikasi Teknologi Hijau Untuk Pemantauan Kualitas Lingkungan Berbasis Struktur Komunitas Mikroba

Hanif

2016

Jurtekling

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Struktur komunitas mikroba memiliki peran yang penting dalam pemantauan kualitas lingkungan.Strategi pengembangan metode analitis yang handal untuk mengkarakterisasi struktur komunitasmikroba masih merupakan sebuah tantangan.Analisis profil respirasi kuinon (RK) merupakan salahsatu metode identifikasi mikroba tanpa kulturisasi yang telah luas digunakan untuk mengkarakterisasistruktur komunitas mikroba. Studi ini bertujuan untuk mengaplikasikan teknologi ekstraksi fluidasuperkritis (EFS) sebagai sebuah teknologi hijau untuk determinasi struktur komunitas mikrobakuinondalam lumpur IPAL. Statistik desain percobaan digunakan dalam studi ini untuk mengoptimasikondisi EFS, dan mengeksplorasi hubungan antara beberapa peubah dan satu atau lebih peubahrespon. Kromatografi cairan ultra performansi (KCUP) dilengkapi detektor foto diode arraydigunakan untuk determinasi secara simultan ubikuinon (UK) dan menakuinon (MK).Ekstraksisuperkritis berfluida CO2 dengan perangkap kartridge fase padat terbukti lebih efektif dan cepatuntuk mengekstraksi RK dibandingkan metode konvensional dengan pelarut organik.Keberhasilanprosedur analitis dari metode ini dibuktikan dengan adanya pengurangan signifikan dari kompleksitasprosedur yang gunakan dan penurunan waktu preparasi sampel.Hasil optimalisasi metode EFS inijuga diuji sensitifitas nya dalam mengkarakterisasi komunitas mikroba berbasis profil kuinon padaberbagai sampel seperti tanah, air kolam, sedimen sungai, lumpur digesi anaerob. Metode ini dapatmenjadi metode rutin dalam pemantauan struktur komunitas mikroba di lingkungan

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Section: Pendahuluanunclassified

Aplikasi Teknologi Hijau Untuk Pemantauan Kualitas Lingkungan Berbasis Struktur Komunitas Mikroba

Hanif

2016

Jurtekling

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“…The analysis was performed at 35 ± 1 ∘ C with a chromatographic run time of 50 min. The autosampler temperature was set at 4.0 ± 1 ∘ C and the sample injection volume was 10 L [13].…”

Section: Ultra Performance Liquid Chromatography Analysismentioning

confidence: 99%

“…They discovered that SFE, with a solid-phase cartridge trap, proved to be a more rapid and effective method for extracting respiratory quinones, compared to a conventional organic solvent extraction method. The method could significantly reduce the complexity in the procedure and time required for sample preparation [13].…”

Section: Introductionmentioning

confidence: 99%

“…SFE has been successfully used as an effective, rapid, and quantitative extraction technique for quinones from activated sludge samples [12,13]. Irvan et al reported that the optimum conditions for extraction of quinones from activated sludge were at a pressure of 25 MPa, a temperature of 55 ∘ C, and 10% (v/v) methanol for a 15 min extraction time [12].…”

Section: Introductionmentioning

confidence: 99%

“…In order to overcome these disadvantages, a sample preparation with supercritical fluid extraction (SFE) has been developed [12,13]. SFE has been successfully used as an effective, rapid, and quantitative extraction technique for quinones from activated sludge samples [12,13].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Supercritical Fluid Extraction of Quinones from Compost for Microbial Community Analysis

Juliasih

Yuan

Sago

et al. 2015

Journal of Chemistry

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Supercritical fluid extraction (SFE) was used to extract quinones from compost to monitor the microbial community dynamics during composting. The 0.3 g of dried compost was extracted using 3 mL min −1 of carbon dioxide (90%) and methanol (10%) at 45 ∘ C and 25 MPa for a 30 min extraction time. The extracted quinones were analysed using ultra performance liquid chromatography (UPLC) with 0.3 mL min −1 of methanol mobile phase for a 50 min chromatographic run time. A comparable detected amount of quinones was obtained using the developed method and an organic solvent extraction method, being 36.06 mol kg −1 and 34.54 mol kg −1 , respectively. Significantly low value of dissimilarity index ( ) between the two methods (0.05) indicated that the quinone profile obtained by both methods was considered identical. The developed method was then applied to determine the maturity of the compost by monitoring the change of quinone during composting. The UQ-9 and MK-7 were predominant quinones in the initial stage of composting. The diversity of quinone became more complex during the cooling and maturation stages. This study showed that SFE had successfully extracted quinones from a complex matrix with simplification and rapidity of the analysis that is beneficial for routine analysis.

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A simple and efficient method for the extraction and separation of menaquinone homologs from wet biomass of Flavobacterium

Wei

Zhao

Liu

et al. 2017

Bioprocess Biosyst Eng

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Menaquinone homologs (MK-n), that is, MK-4, MK-5, and MK-6, can be produced by the fermentation of Flavobacterium. In this study, we proposed a simple and efficient method for the extraction of menaquinones from wet cells without the process of drying the biomass. Meanwhile, a rapid and effective solution for the separation of menaquinone homologs was developed using a single organic solvent, which was conducive to the recovery of the solvent. The results showed that the highest yield was obtained with pretreatment using absolute ethanol at a ratio of 6:1 (v/m) for 30 min and then two extractions of 30 min each using methanol at a ratio of 6:1 (v/m). The recovery efficiency of the menaquinones reached to 102.8% compared to the positive control. The menaquinone homologs were effectively separated using methanol as eluent at a flow rate of 0.52 mL/min by a glass reverse-phase C18 silica gel column with a height-to-diameter ratio of 5.5:1. The recovery of menaquinones achieved was 99.6%. In conclusion, the methods for extraction and separation of menaquinone homologs from wet Flavobacterium cells were simple and efficient, which makes them suitable not only on a laboratory scale but also for application on a large scale.

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Supercritical Fluid Extraction and Ultra Performance Liquid Chromatography of Respiratory Quinones for Microbial Community Analysis in Environmental and Biological Samples

Cited by 12 publications

References 34 publications

Aplikasi Teknologi Hijau Untuk Pemantauan Kualitas Lingkungan Berbasis Struktur Komunitas Mikroba

Aplikasi Teknologi Hijau Untuk Pemantauan Kualitas Lingkungan Berbasis Struktur Komunitas Mikroba

Supercritical Fluid Extraction of Quinones from Compost for Microbial Community Analysis

A simple and efficient method for the extraction and separation of menaquinone homologs from wet biomass of Flavobacterium

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