Supercritical Fluid Extraction of Bioavailable Amino Acids from Soils and Their Liquid Chromatographic Determination with Fluorometric Detection

Lojková, Lea; Klejdus, Bořivoj; Formánek, Pavel; Kubán,

doi:10.1021/jf061255k

Cited by 7 publications

(8 citation statements)

References 23 publications

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“…on soil microfl ora. Qualitative composition of individual compound divisions (sugars, amino acids and organic acids) and ratio of their occurrence was examined by Formánek et al (2009), who proved the dominance of aspartic acid, arginine, alanine and glutamic acid in M × g root exudates, when these amino acids were also confi rmed as dominant in soil solution gathered from various types of ecosystems (Lojková et al, 2006).…”

Section: Organic and Inorganic Pollutant Bioremediationmentioning

confidence: 99%

Giant miscanthus (Miscantus × Giganteus Greef Et Deu.) – A Promising Plant for Soil Remediation: A Mini Review

Figala¹,

Vranová²,

Rejšek³

et al. 2015

Acta Univ. Agric. Silvic. Mendelianae Brun.

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Giant miscanthus (Miscanthus × giganteus Greef et Deu.) is a perennial rhizomatous grass with C4 type photosynthesis, which is distinctive by its resistance to cold temperatures when maintaining a suffi cient photosynthesis rate. We revised potential of Miscanhus for use in soil bioremediation, especially from biological point of view. Translocation rate from roots to aerial part is low in general, but Miscanthus is able to grow even on highly contaminated soils without artifi cial fertilization. We also discussed the role of root exudates in pollutant immobilization, chelation and uptake. Commetabolism of polycyclic aromatic hydrocarbons with assistance of soil microbes shows promising results and signifi cant reduction of tetracyclic PAHs in soil. Miscanthus is therefore suitable for immobilization of inorganic pollutants in soil and removal of organic pollutants, which makes it suitable to create buff er zones for surface waterway protection, stabilization of heavily contaminated substrates (e.g. reclaimed burrows of mining industry and sedimentation pools). According to low content of pollutants in aerial biomass the harvested plant material is deemed safe for further agricultural or industrial use.

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Section: Organic and Inorganic Pollutant Bioremediationmentioning

confidence: 99%

Giant miscanthus (Miscantus × Giganteus Greef Et Deu.) – A Promising Plant for Soil Remediation: A Mini Review

Figala¹,

Vranová²,

Rejšek³

et al. 2015

Acta Univ. Agric. Silvic. Mendelianae Brun.

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“…A small amount of 0.1 M HCl was added as an in situ modifier in some cases. Preparation of model samples is described in detail in [16] and was performed without substantial changes to the original method. The samples were used only to verify proper functioning of both the method and the instrument for the analytes of interest under the given conditions.…”

Section: Sample Preparationmentioning

confidence: 99%

“…The method has already been validated [16] and the tests were performed just to verify the chromatographic system parameters. However, both RSDs and the recovery of HPLC method were very good (0.5 -1.7% and 99.2 -100.8%, respectively) in comparison with the spread of SFE results (usually below 5.5% under selected condition and even worse for not fully optimized series, especially at lower concentrations).…”

Section: Analytical Parametersmentioning

confidence: 99%

“…The previously described optimal conditions for the extraction of amino acids from model spiked samples were applied [16]. However, a dry leaf matrix is com- pletely different from wet soil samples used in [16] as real matrix.…”

Section: Supercritical Fluid Extraction Conditionsmentioning

confidence: 99%

“…One year later, the same group [16] applied a supercritical fluid extraction (SFE) technique for isolation of free amino acids from soil samples.…”

Section: Introductionmentioning

confidence: 99%

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Supercritical fluid extraction of amino acids from birch (Betula pendula Roth) leaves and their liquid chromatographic determination with fluorimetric detection

Klejdus

Lojková

Kula

et al. 2008

J of Separation Science

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A method for supercritical fluid extraction (SFE) of amino acids was adapted and optimal experimental conditions were selected for a matrix consisting of dry leaves. The matrix-dependent SFE method uses a mixture of MeOH-H(2)O-acetonitrile (10:10:1 v/v/v) as a modifier (0.5 mL in situ, 300 muL on-line) at 70 degrees C and 40 MPa and no HCl is needed as an entrainer. The amino acids were quantified using high-performance liquid chromatography with fluorimetric detection (HPLC/FLD) after gradient elution on Zorbax Eclipse AAA columns (4.6x150 mm, 3.5 mum) with aqueous Na(2)HPO(4 )buffer of pH 7.8 and ACN-MeOH-water as a mobile phase. In comparison with Soxhlet extraction, SFE gave higher recovery and selectivity, but it required longer extraction time (90 min) and it was more labor-intensive (clean-up step after the pre-concentration). Both methods should be used separately or in combination according to the matrix, number of samples, and levels of ballast compounds.

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Simultaneous determination of ethyl carbamate and 4-(5-)methylimidazole in yellow rice wine and soy sauce by gas chromatography with mass spectrometry

Zhang

Wang

et al. 2014

J. Sep. Science

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We developed a new method, based on alkaline diatomite solid-phase extraction followed by gas chromatography with mass spectrometry, for the simultaneous determination of the toxic contaminants ethyl carbamate (EC) and 4-(5-)methylimidazole (4-MEI) in yellow rice wine and soy sauce. The optimal extraction conditions were defined. With the application of alkaline diatomite solid-phase extraction, damage to the capillary column by organic acids was greatly reduced. With deuterated EC used as the internal standard, the linearity of the calibration curves for EC and 4-MEI was good with correlation coefficient above 0.99. In a spiked experiment with EC and 4-MEI in yellow rice wine and soy sauce, recovery of the added EC was 80.5-102.5% and that of 4-MEI was 78.3-92.8%. The limit of quantification and limit of detection for EC were 6.0 and 2.0 μg/kg, respectively, and for 4-MEI were 15.0 and 5.0 μg/kg, respectively. The validation results demonstrate that the method is fast, simple, and selective, and therefore is suitable for simultaneously determining the presence of EC and 4-MEI in fermented food.

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Supercritical Fluid Extraction of Bioavailable Amino Acids from Soils and Their Liquid Chromatographic Determination with Fluorometric Detection

Cited by 7 publications

References 23 publications

Giant miscanthus (Miscantus × Giganteus Greef Et Deu.) – A Promising Plant for Soil Remediation: A Mini Review

Giant miscanthus (Miscantus × Giganteus Greef Et Deu.) – A Promising Plant for Soil Remediation: A Mini Review

Supercritical fluid extraction of amino acids from birch (Betula pendula Roth) leaves and their liquid chromatographic determination with fluorimetric detection

Simultaneous determination of ethyl carbamate and 4-(5-)methylimidazole in yellow rice wine and soy sauce by gas chromatography with mass spectrometry

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