Superficial contamination of bovine carcasses by Escherichia coli O157:H7 in a slaughterhouse in Normandy (France)

Guyon, R.; Dorey, F.; Malas, J.P.; Grimont, Francine; Foret, J.; Rouvière, B.; Collobert, J. F.

doi:10.1016/s0309-1740(01)00036-5

Cited by 17 publications

(10 citation statements)

References 15 publications

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“…Similar to our study, non-pathogenic E. coli O 157:H7 strains (without toxins factors) have also been identified from feces or beef carcass (Guyon et al, 2001;Rogerie et al, 2001). Cagney et al (2004) studied the prevalence of E. coli O157:H7 in ground beef and beef burger (n=1533) from supermarkets and butcher shops.…”

Section: Discussionsupporting

confidence: 76%

Prevalence and antimicrobial susceptibility of Escherichia coli O157:H7 on beef cattle slaughtered in Amman abattoir

2013

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Section: Discussionsupporting

confidence: 76%

Prevalence and antimicrobial susceptibility of Escherichia coli O157:H7 on beef cattle slaughtered in Amman abattoir

2013

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“…E. coli O157 or E. coli O157: H7 isolates obtained from cattle carcasses or feces have also been found to contain at least one of the stx1 , stx2 , eaeA , hylA and fliC h7 genes [5,7,22,25,29,35,38,39,41]. In contrast to other countries, there has been only one study of carcasses reported in Turkey [21].…”

Section: Discussionmentioning

confidence: 99%

“…There have been many studies conducted to determine E. coli O157 and E. coli O157:H7 in only carcass or rectal samples at abattoirs worldwide, including Turkey [5,7,22,41]. However, to the best of our knowledge, there has been no attempt to detect E. coli O157 and E. coli O157:H7 in both rectal and carcass samples from the same animal.…”

Section: Introductionmentioning

confidence: 99%

Detection ofEscherichia coliO157 andEscherichia coliO157:H7 by the immunomagnetic separation technique andstx1andstx2genes by multiplex PCR in slaughtered cattle in Samsun Province, Turkey

Inat

Sırıken

2010

J Vet Sci

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This study was conducted to investigate the presence of Escherichia (E.) coli O157 and E. coli O157:H7 and stx1 and stx2 genes on cattle carcasses and in rectal samples collected from Samsun Province of Turkey. A total of 200 samples collected from cattle carcasses and the rectal contents of 100 slaughtered cattle from two commercial abattoirs were tested using the immunomagnetic separation technique and multiplex PCR methods. E. coli O157 and E. coli O157:H7 were detected in 52 of the 200 samples (26%) tested. Of the positive samples, 49 were E. coli O157 and three were E. coli O157:H7. The E. coli O157 strain was isolated from 24 carcasses and 25 rectal samples, while E. coli O157:H7 was isolated from two carcasses and one rectal sample. Of the 49 samples positive for E. coli O157, 32 were from the rectal and carcass samples of the same animal, while two E. coli O157:H7 isolates were obtained from rectal swabs and carcasses of the same animal. The stx1 and stx2 genes were both detected in 35 E. coli O157 isolates and one E. coli O157:H7 isolate, but the stx2 gene was only detected alone in two E. coli O157 isolates. Overall, 16 carcasses tested positive for E. coli O157 and one carcass tested positive for E. coli O157:H7 based on both carcass and rectal samples. Overall, the results of this study indicate that cattle carcasses pose a potential risk to human health due to contamination by E. coli O157 and E. coli O157:H7 in the feces.

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“…In Switzerland, Fantelli and Stephan (2001) reported 2.3% prevalance of E. coli O157: H7 in 211 ground beef samples. Guyon et al (2001) reported that they isolated only one E. coli O157: H7 only from 225 beef samples. In Turkey, the prevalence of the agent was investigated in various studies.…”

Section: Resultsmentioning

confidence: 99%

Characterization and Antimicrobial-Resistance Profile of Escherichia coli O157 and O157: H7 Isolated from Modified Atmosphere Packaged Meat Samples

Çadırcı

Gücükoğlu

Güzel

et al. 2017

Turkish JAF Sci.Tech.

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Shiga-like toxin producing Escherichia coli is still an important public issue which causes extremely dangerous health problems. This study was planned in order to examine the inhibitory effect of Modified Atmosphere Packaging application on E. coli O157 and O157: H7. The purposes of the present study were to detect E. coli O157 and O157: H7 strains from ground and cubed beef. A total of 100 MAP cattle meat products (50 minced meat, 50 meat cubes) were collected from the markets and butchers in Samsun province between May and October 2013. According to results, 1(1/50-2%) E. coli O157 and 1(1/50-2%) E. coli O157: H7 strains isolated from 50 ground beef samples, while 1 (1/50-2%) E. coli O157 strain was identified from 50 cubed beef samples. It was determined that E. coli O157 isolate obtained from the MAP ground beef carried stx 1, stx 2 genes; E. coli O157: H7 isolate carried stx 1, stx 2, eaeA and hylA genes while E. coli O157 isolate obtained from the MAP cubed meat only carried the stx 2 gene. In antibiogram test, both E. coli O157 isolates were resistant to streptomycin and one E. coli O157: H7 isolate was resistant to streptomycin, cephalothin and tetracycline. As a consequence; in order to protect public health, products should be kept in proper hygienic and technical conditions during sale and storage and use of uncontrolled antibiotics should be avoided. H7 ile, 50 adet MAP sığır kuşbaşı örneğinin 1'inin (1/50-%2) ise E. coli O157 ile kontamine olduğu belirlenmiştir. Yapılan genotiplendirme sonucunda MAP sığır kıyma örneklerinden elde edilen E. coli O157 izolatının stx1, stx2, E. coli O157: H7 izolatının ise stx1, stx2, eaeA ve hlyA gen bölgelerini taşıdığı, MAP sığır kuşbaşı örneklerinden elde edilen E. coli O157 izolatının ise sadece stx2 gen bölgesini taşıdığı belirlenmiştir. Fenotipik antibiyotik profil analizinde E. coli O157 izolatlarının her ikisinin yalnızca streptomisine karşı dirençli olduğu bununla beraber E. coli O157: H7 izolatının ise streptomisin, sefalothin ve tetrasiklin antibiyotiklerine karşı dirençli olduğu saptanmıştır. Sonuç olarak halk sağlığının korunması amacıyla ürünlerin uygun teknik ve hijyenik şartlarda satışa sunulması ve muhafaza edilmesine dikkat edilmesi, ayrıca izolatların antibiyotiklere direnç göstermesi nedeniyle kontrolsüz antibiyotik kullanımının önlenmesi önerilmektedir.

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Superficial contamination of bovine carcasses by Escherichia coli O157:H7 in a slaughterhouse in Normandy (France)

Cited by 17 publications

References 15 publications

Prevalence and antimicrobial susceptibility of Escherichia coli O157:H7 on beef cattle slaughtered in Amman abattoir

Prevalence and antimicrobial susceptibility of Escherichia coli O157:H7 on beef cattle slaughtered in Amman abattoir

Detection ofEscherichia coliO157 andEscherichia coliO157:H7 by the immunomagnetic separation technique andstx1andstx2genes by multiplex PCR in slaughtered cattle in Samsun Province, Turkey

Characterization and Antimicrobial-Resistance Profile of Escherichia coli O157 and O157: H7 Isolated from Modified Atmosphere Packaged Meat Samples

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