Superior fluorescent probe for detection of potassium ion

Chen, Zhengbo; Tan, Lulu; Hu, Liangyu; Luan, Yunxia

doi:10.1016/j.talanta.2015.06.015

Cited by 23 publications

(9 citation statements)

References 36 publications

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“…In this study, PG was utilized as a fluorescent probe owing to its high specificity and sensitivity for binding to dsDNA. Upon binding to dsDNA, fluorescence of PG increases more than 1000-fold, while the free PG exhibits no fluorescence [23,24].…”

Section: Resultsmentioning

confidence: 99%

A fluorescent aptasensor based on a DNA pyramid nanostructure for ultrasensitive detection of ochratoxin A

et al. 2016

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Analytical techniques for detection of ochratoxin A (OTA) in food products and blood serum are of great significance. In this study, a fluorescent aptasensor was developed for sensitive and specific detection of OTA, based on a DNA pyramid nanostructure (DPN) and PicoGreen (PG) dye. The designed aptasensor inherits characteristics of DPN, such as high stability and capacity for PG loading. PG, as a fluorescent dye, could bind to double-stranded DNA (dsDNA). In the absence of OTA, the pyramid structure of DPN remains intact, leading to a very strong fluorescence emission. Because of higher affinity of aptamer for its target relative to its complementary strand, upon addition of target, the pyramid structure of DPN is disassembled, leading to a weak fluorescence emission. The presented aptasensor showed high specificity toward OTA with a limit of detection (LOD) as low as 0.135 nM. Besides, the designed sensing strategy was successfully utilized to recognize OTA in serum and grape juice with LODs of 0.184 and 0.149 nM, respectively.

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Section: Resultsmentioning

confidence: 99%

A fluorescent aptasensor based on a DNA pyramid nanostructure for ultrasensitive detection of ochratoxin A

et al. 2016

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“…Our group has developed al abel-free OTAa ssay using the luminescent G-quadruplex-selective iridium(III) probe. [49] (Figure 3b) The oligonucleotide sequence ON1, which contains the OTAa ptamer( 5 '-GATCG 3 TGTG 3 TG 2 CGTA 3 G 3 AGCATCG 2 ACA-3'), is initially hybridized with ap artially complementary DNA strand ON2 (5'-C 2 ACAC 3 GATC-3'). The addition of OTAc auses dissociation of the ON1-ON2 duplex owing to the formation of the OTAa ptamer G-quadruplex.…”

Section: G-quadruplex Aptamer-based Probes For Ochratoxin Amentioning

confidence: 99%

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2017

ChemPlusChem

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“…Recently, Chen and co‐workers reported a label‐free strategy for K + detection that utilizes PicoGreen as a fluorescent duplex probe . PicoGreen is a small molecule organic dye that interacts with duplex DNA to produce an enhanced luminescence signal (Figure c).…”

Section: Luminescent G‐quadruplex Aptamer‐based Probes For Metal Ionsmentioning

confidence: 99%

“…Our group has developed a label‐free OTA assay using the luminescent G‐quadruplex‐selective iridium(III) probe . (Figure b) The oligonucleotide sequence ON1, which contains the OTA aptamer (5′‐GATCG 3 TGTG 3 TG 2 CGTA 3 G 3 AGCATCG 2 ACA‐3′), is initially hybridized with a partially complementary DNA strand ON2 (5′‐C 2 ACAC 3 GATC‐3′).…”

Section: Luminescent G‐quadruplex Aptamer‐based Probes For Small Molementioning

confidence: 99%

“…In this context of the label‐free strategy, we note that both organic (e.g., NMM, CV) and inorganic (e.g., iridium(III) complexes) G‐quadruplex probes have been utilized for the development of G‐quadruplex aptamer‐based assays. The paramount goal of any G‐quadruplex probe is to achieve high selectivity for G‐quadruplex DNA over other DNA conformations, and thus we envision that further gains in sensitivity and selectivity of these assays can be achieved as superior G‐quadruplex dyes are developed.…”

Section: Outlook and Summarymentioning

confidence: 99%

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Utilization of G‐Quadruplex‐Forming Aptamers for the Construction of Luminescence Sensing Platforms

Wang

Mao

et al. 2016

ChemPlusChem

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Aptamers are nucleic acid sequences that can recognize and bind to analytes with high affinity and selectivity. Intriguingly, a number of aptamers undergo a conformational change within the G‐quadruplex motif upon ligand binding. This Minireview aims to highlight interesting examples of luminescent G‐quadruplex aptamer‐based probes that have been developed in recent years. Various mechanisms and sensing modes are described, and the outlook and future directions of this field are also discussed.

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Superior fluorescent probe for detection of potassium ion

Cited by 23 publications

References 36 publications

A fluorescent aptasensor based on a DNA pyramid nanostructure for ultrasensitive detection of ochratoxin A

A fluorescent aptasensor based on a DNA pyramid nanostructure for ultrasensitive detection of ochratoxin A

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Utilization of G‐Quadruplex‐Forming Aptamers for the Construction of Luminescence Sensing Platforms

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