Superior Survival and Proliferation after Transplantation of Myoblasts Obtained from Adult Mice Compared with Neonatal Mice

Lee-Pullen, Tracey F.; Bennett, Alayne L.; Beilharz, Manfred W.; Grounds, Miranda D.; Sammels, Leanne M.

doi:10.1097/01.tp.0000137936.75203.b4

Cited by 22 publications

(17 citation statements)

References 26 publications

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“…Myoblasts isolation and purification rendered them largely incapable of proliferation and fusion in recipient environment: probably the isolation procedure produced subtle changes in the myoblasts that rendered them either more visible to the host immune system or less viable for fusion in vivo [27]. Interestingly, Lee-Pullen et al studied the transplantation efficiency of myoblasts isolated from both adult and neonatal mice [28]. The majority of MTT used donor myoblasts from neonate tissue on the basis of increased yield of activated precursor cells at this young age.…”

Section: Myoblastsmentioning

confidence: 97%

“…Other groups isolated donor myoblasts from more mature muscle at a time when the myoblasts are quiescent, as they are closer to the source of donor cells from adult tissues used in clinical studies. As they demonstrated that adult myoblasts showed sustained survival and striking proliferation in comparison with neonate-derived myoblasts, it became clear that the source of donor myoblasts could have a pronounced effect on the efficacy of the procedure [28]. Moreover, problems regarding inadequate immunosuppression arose.…”

Section: Myoblastsmentioning

confidence: 98%

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The Role of Stem Cells in Muscular Dystrophies

Meregalli

Farini

Colleoni

et al. 2012

CGT

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Muscular dystrophies are heterogeneous neuromuscular disorders of inherited origin, including Duchenne muscular dystrophy (DMD). Cell-based therapies were used to promote muscle regeneration with the hope that the host cells repopulated the muscle and improved muscle function and pathology. Stem cells were preferable for therapeutic applications, due to their capacity of self-renewal and differentiative potential. In the last years, encouraging results were obtained with adult stem cells to treat muscular dystrophies. Adult stem cells were found into various tissues of the body and they were able to maintain, generate, and replace terminally differentiated cells within their own specific tissue because of cell turnover or tissue injury. Moreover, it became clear that these cells could participate into regeneration of more than just their resident organ. Here, we described multiple types of muscle and non muscle-derived myogenic stem cells, their characterization and their possible use to treat muscular dystrophies. We also underlined that most promising possibility for the management and therapy of DMD is a combination of different approaches, such as gene and stem cell therapy.

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Section: Myoblastsmentioning

confidence: 97%

Section: Myoblastsmentioning

confidence: 98%

The Role of Stem Cells in Muscular Dystrophies

Meregalli

Farini

Colleoni

et al. 2012

CGT

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“…However, another study reported only a slight difference between the numbers of dystrophin positive fibres after injection of late pre-plate (PP6), or early passage primary myoblasts, although it should be noted that the MDSC were injected after many passages in vitro (29). We have recently observed increased survival and also proliferation in a population of late pre-plate cells (equivalent to MDSC) extracted from adult mice (30). Following an initial loss of 50% of male donor cells by 24 h, no further decrease was observed for up to 3 weeks post-transplantation.…”

Section: Muscle-derived Stem Cellsmentioning

confidence: 99%

Muscle-derived stem cells: Implications for effective myoblast transfer therapy

Lee-Pullen

Grounds

2005

IUBMB Life (International Union of Biochemistry and Molecular B

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SummaryStem cells have been proposed as a wonder solution for tissue repair in many situations and have attracted much attention in the media for both their therapeutic potential and ethical implications. In addition to the excitement generated by embryonic stem cells, research has now identified a number of stem cells within adult tissues which pose much more realistic targets for therapeutic interventions. Myoblast transfer therapy (MTT) has long been viewed as a potential therapy for the debilitating muscle-wasting disorder Duchenne Muscular Dystrophy. This technique relies on the transplantation of committed muscle precursor cells directly into the muscle fibres but has had little success in clinical trials. The recent discovery of a population of cells within adult muscle with stem cell-like characteristics has interesting implications for the future of such putative cell transplantation therapies. This review focuses on the characterization and application of these potential muscle-derived stem cells (MDSC) to MTT. IUBMB Life, 57: 731 -736, 2005

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“…A number of studies have examined transplantations of normal stem cells such as hematopoietic stem cells (HSCs1), myoblasts, and stem cells derived from other tissue types as possible treatments for DMD and as a gene delivery system for therapeutic recombinant proteins (Camargo et al 2003; Huard et al 2003; Lee-Pullen et al 2004; Parker et al 2008; Partridge et al 1998; Torrente et al 2004). The disease phenotype of the mdx mouse model also shows improvement after the injection, either intramuscular or intravenous (Gregorevic et al 2004; Gregorevic and Chamberlain 2003; Liu et al 2005), of viral vectors encoding full-length or truncated but functional dystrophins (DelloRusso et al 2002; Dudley et al 2004; Harper et al 2002b).…”

Section: Treatmentsmentioning

confidence: 99%

“…Fusion of mononucleated myoblasts either into multinucleated muscle fibers or with existing myofibers is a normal part of muscle repair. Normal myoblasts are able to fuse with dystrophic myoblasts to form myotubes that express dystrophin in vitro (Huard et al 2003; Lee-Pullen et al 2004). Similarly, intramuscular injection of myoblasts from normal donor mice reconstituted muscle fibers and led to dystrophin expression in mdx mice despite the rapid death of a high percentage of donor cells (Beauchamp et al 1999; Jejurikar and Kuzon 2003; Lee-Pullen et al 2004; Skuk et al 2004).…”

Section: Treatmentsmentioning

confidence: 99%

Gene Therapy in Large Animal Models of Muscular Dystrophy

et al. 2009

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The muscular dystrophies are a group of genetically and phenotypically heterogeneously inherited diseases characterized by progressive muscle wasting, which can lead to premature death in severe forms such as Duchenne muscular dystrophy (DMD). In many cases they are caused by the absence of proteins that are critical components of the dystrophin-glycoprotein complex, which links the cytoskeleton and the basal lamina. There is no effective treatment for these disorders at present, but several novel strategies for replacing or repairing the defective gene are in development, with early encouraging results from animal models. We review these strategies, which include the use of stem cells of different tissue origins, gene replacement therapies mediated by various viral vectors, and transcript repair treatments using exon skipping strategies. We comment on their advantages and on limitations that must be overcome before successful application to human patients. Our focus is on studies in a clinically relevant large canine model of DMD. Recent advances in the field suggest that effective therapies for muscular dystrophies are on the horizon. Because of the complex nature of these diseases, it may be necessary to combine multiple approaches to achieve a successful treatment.

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Superior Survival and Proliferation after Transplantation of Myoblasts Obtained from Adult Mice Compared with Neonatal Mice

Abstract: Myoblasts derived from neonatal mice were inferior for transplantation, and early passage donor myoblasts from adult mice are recommended for MTT in this model.

Cited by 22 publications

References 26 publications

The Role of Stem Cells in Muscular Dystrophies

The Role of Stem Cells in Muscular Dystrophies

Muscle-derived stem cells: Implications for effective myoblast transfer therapy

Gene Therapy in Large Animal Models of Muscular Dystrophy

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