Superovulatory response and embryo development in ewes treated with two doses of bovine somatotropin

Carrera‐Chávez, José Maria; Hernández-Cerón, Joel; López-Carlos, Marco Antonio; Lozano-Domínguez, Renato Raúl; Molinar, Francisco; Echavarría-Cháirez, Francisco Guadalupe; Bañuelos-Valenzuela, Rómulo

doi:10.1016/j.anireprosci.2014.10.009

Cited by 5 publications

(4 citation statements)

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“…We noticed an increase of IGF-1 levels in serum and GCs in GH-treated lambs, supporting the function of GH in promoting the synthesis of IGF-1 in vivo [ 17 , 21 ]. We found that IGF-1 indeed regulated the expression of genes related to cell proliferation and apoptosis in GCs cultured in vitro.…”

Section: Discussionsupporting

confidence: 65%

“…Many previous studies have suggested that GH could improve the reproductive performance in mammals [ 27 , 28 ]. In superovulated ewes, although the regimen and dosage were different, GH treatment allowed the enhancement of the pool of gonadotrophin-responsive follicles present in the ovaries [ 29 ], increased the rates of fertilization and blastocyst development [ 19 ], and enhanced the proportion of transferable embryos [ 17 ]. In estrus-synchronized ewes, giving 50 mg GH at the start of synchronization treatment and at the time of artificial insemination increased the proportion of multiple births and prolificacy [ 20 , 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…For instance, treatment with GH can significantly shorten the gonadotropin stimulation duration before oocyte retrieval and increase the number of competent oocytes and clinical pregnancy rate in poor ovarian responders [ 15 , 16 ]. In sheep, the utility of GH has been reported in estrus synchronization and superovulation of adult ewes for enhancing the follicle development and embryo production [ 17 – 22 ]. These studies support the potential role of GH in improvement of the reproductive performance in mammals.…”

Section: Introductionmentioning

confidence: 99%

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Growth hormone treatment improves the development of follicles and oocytes in prepubertal lambs

Liu

Zhang

et al. 2023

J Ovarian Res

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Background When prepubertal lambs are superovulated, the ovarian response to gonadotropin stimulation has great individual difference and the collected oocytes have lower developmental ability than that of adult ewes. Over the years, growth hormone (GH) has been used in assisted reproduction because it can improve the reproductive performance in humans and animals. However, the effect of GH on ovaries and oocytes of prepubertal lambs remains unclear. Methods Before and during follicle-stimulating hormone (FSH) superovulation of prepubertal lambs (4‒6-week-old), the lambs were treated with high (50 mg) or low dose (25 mg) of ovine GH in a long (5 days) or short (2 days) period. The recovered oocytes were used for in vitro maturation and fertilization, and several parameters of oocyte quality and development capacity were evaluated. The possible underlying mechanisms of GH action were explored by analysis of granulosa cell (GC) transcriptome, ovarian proteome and follicular fluid metabolome. Results Treatment of lambs with 50 mg GH over 5 days (long treatment) potentially promoted the response of lambs to superovulation and improved the development capacity of retrieved oocytes, consequently increasing the high quality embryo yield from lambs. A number of differently expressed genes or proteins were found in ovaries between GH-treated and untreated lambs. Cellular experiments revealed that GH reduced the oxidative stress of GCs and promoted the GC proliferation probably through activation of the PI3K/Akt signaling pathway. Finally, analysis of follicular fluid metabolome indicated that GH treatment altered the abundance of many metabolites in follicular fluid, such as antioxidants and fatty acids. Conclusions GH treatment has a beneficial role on function of lamb ovaries, which supports the development of follicles and oocytes and improves the efficiency of embryo production from prepubertal lambs.

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Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Growth hormone treatment improves the development of follicles and oocytes in prepubertal lambs

Liu

Zhang

et al. 2023

J Ovarian Res

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“…En rumiantes, se ha demostrado que una dosis única de rBST mejora el desarrollo embrionario si se aplica al momento de la inseminación artificial (Mejía et al 2012), incrementando el IFNT (vacas lecheras) y aumenta la tasa de concepción (Ribeiro et al 2014). Asimismo, en programas de ovulación múltiple y transferencia de embriones (OMTE) se ha mejorado la tasa de concepción cuando se aplica la rBST al momento del estro en vacas de leche (Ribeiro et al 2014), no así en ovejas (Carrera-Chávez et al 2014). Tanto en vacas como en ovejas se ha mencionado la importancia de iniciar el tratamiento de la rBST para mejorar la tasa de ovulación como el porcentaje de cuates (Safdar y Sadeghi 2016).…”

Section: Introductionunclassified

Respuesta ovulatoria y embrionaria a la somatotropina bovina recombinante en cabras superovuladas con FSHp

Sánchez-Dávila

2019

Ecosist. Recur. Agropec.

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Nota científica recibida: 25 de agosto de 2017 aceptado: 07 de septiembre de 2018 RESUMEN. El objetivo del estudio fue evaluar el efecto de la somatotropina bovina recombinante (rBST) en la actividad ovárica, cantidad y calidad de embriones transferibles en cabras durante el anestro estacional. Se utilizaron 30 cabras multíparas sincronizadas con protocolo corto de CIDR y superovuladas con FSHp, asignadas a tres tratamientos (n = 10): T1 = control, sin rBST, T2 = 100 mg rBST y T3 = 200 mg rBST. Las variables cuantitativas se analizaron mediante prueba de Kruskal-Wallis; el porcentaje de estro y de embriones recuperados, mediante prueba de Chi-cuadrada. Las cabras del T3 tuvieron mayor número de CL, seguidas del T2 (p = 0.002). El número de embriones recuperados y de blastocitos fue mayor en T3 (p = 0.001). El número de mórulas fue mayor en T1 (p = 0.001). La aplicación de rBST al inicio del estro incrementa la actividad ovárica y la cantidad de embriones transferibles en cabras.

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Lambing rate and prolificacy in inseminated hair sheep treated with bovine somatotropin

Carrera‐Chávez

Hernández-Cerón

López-Carlos

et al. 2016

Trop Anim Health Prod

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This study evaluated whether the administration of 50 and 100 mg bovine somatotropin (bST) at the start of estrous synchronization and at the time of artificial insemination improves lambing rate and prolificacy in hair sheep. Four hundred eighty adult hair ewes (Pelibuey, Blackbelly, Dorper, Katahdin, and their crosses) were synchronized with intravaginal sponge containing 40 mg of fluorogestone acetate. On the day of sponge insertion, ewes were assigned to three treatments: the bST-100 treatment (n = 156) received 100 mg bST at the start of synchronization (d 0) and at the time of insemination (d 14), the bST-50 treatment (n = 159) received 50 mg bST in the same schedule as the previous group, and the control (n = 165) did not receive any bST. Lambing rate and percentage of multiple births were analyzed using the GENMOD procedure of SAS. Prolificacy data were analyzed using the MIXED procedure of SAS. The IGF-1 and insulin concentrations were analyzed with ANOVA for repeated measures. The bST application did not affect the lambing rate (P = 0.06). The proportion of ewes with multiple births (P = 0.01) and prolificacy (P = 0.04) were higher in the bST-50 (54.3% and 1.57 ± 0.1) than the bST-100 (18.2% and 1.25 ± 0.1) and control (33.3% and 1.28 ± 0.1) groups. The IGF-1 and insulin concentrations were higher (P < 0.05) in the bST-treated groups, but the insulin concentration was higher (P = 0.001) in the bST-100 group than in the bST-50 group. The administration of 50 or 100 mg bST at the start of synchronization and at the time of artificial insemination does not increase lambing rate. However, the dose of 50 mg increased the proportion of multiple births and prolificacy.

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Superovulatory response and embryo development in ewes treated with two doses of bovine somatotropin

Cited by 5 publications

References 38 publications

Growth hormone treatment improves the development of follicles and oocytes in prepubertal lambs

Growth hormone treatment improves the development of follicles and oocytes in prepubertal lambs

Respuesta ovulatoria y embrionaria a la somatotropina bovina recombinante en cabras superovuladas con FSHp

Lambing rate and prolificacy in inseminated hair sheep treated with bovine somatotropin

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