Superoxide Dismutase Activity in Lymphocytes and Polymorphonuclear Cells of Diabetic Patients

Abstract

Copper-zinc Superoxide dismutase (Cu,Zn-superoxide dismutase) activity was evaluated in lymphocytes and polymorphonuclear cells of insulin-dependent (n = 33) and non-insulin-dependent (n = 34) diabetic patients. A commercial method for the measurement of Superoxide dismutase activity was adapted for use on a discrete analyser and evaluated for interference by other antioxidants with Superoxide anion-scavenging properties. In comparison to healthy control subjects (n = 32), a significantly lower Cu,Zn-superoxide dismutase activity was found in both lymphocytes and polymorphonuclear cells of insulin-dependent (2.08 ± 0.58 vs. 1.70 ± 0.46 U/mg protein, p < 0.05, and 1.06 ± 0.46 vs. 0.64 ± 0.40 U/mg protein, p < 0.001, respectively) and non-insulin-dependent diabetic patients (2.08 ± 0.58 vs. 1.61 ± 0.48 U/mg protein, p < 0.01, and 1.06 ± 0.46 vs. 0.53 ± 0.24 U/mg protein, p < 0.001, respectively). There was a weak, but significant negative correlation between age and Cu,Zn-superoxide dismutase activity in lymphocytes and polymorphonuclear cells (r = -0.22 and r = -0.28, p < 0.05, respectively), whereas no influence of gender, diabetes duration and glycaemic control was observed. The results indicate that diabetes mellitus could elicit a significant disturbance in Superoxide anion-scavenging potential of lymphocytes and polymorphonuclear cells.

Antioxidant activity of n-butanol extract of celery (Apium graveolens) seed in streptozotocin-induced diabetic male rats

“…SOD and CAT are the two major scavenging enzymes that remove toxic free radicals. Previous studies have reported that the activity of SOD is low in diabetes mellitus (Vucic et al, 1997). Administration of n-BF increased the activity of these enzymes and may help to control free radical, as n-BF has been reported to be rich in flavonoids, a well known antioxidant (Middleton et al, 2000) which scavenges the free radical generated during diabetes.…”

Antioxidant effect of tetrahydrocurcumin in streptozotocin–nicotinamide induced diabetic rats

“…SOD and CAT are the two major scavenging enzymes that remove toxic free radicals in vivo. Previous studies have reported that the activity of SOD is low in diabetes mellitus (Vucic et al, 1997;Feillet-Coudray et al, 1999). A decrease in the activity of these antioxidants can lead to an excess availability of superoxide anion O 2 − and hydrogen peroxide in biological systems, which in turn generate hydroxyl radicals, resulting in initiation and propagation of lipid peroxidation (Kumuhekar and Katyane, 1992).…”

Anti-Oxidative Effect of Cassia auriculata on Streptozotocin Induced Diabetic Rats

“…Diabetic state is shown to be associated with depletion of antioxidants [25]. Vucic et al [26] reported that the activity of SOD is low in diabetes mellitus. Decreased catalase activity in plasma and tissues of streptozotocin diabetic rats, may be due to increased utilization to scavenge the toxic products of lipid peroxidation or decreased availability of H 2 O 2 , the substrate for catalase.…”