Superoxide Dismutase (SOD) Activity in Cryopreserved Semen of Itik Pinas-Khaki (Anas platyrhynchos L.)

Abstract

Cryopreservation induces oxidative stress on sperm due to an increase in the number of reactive oxygen species (ROS), thereby resulting in decreased sperm quality. ROS's destructive potential is normally counteracted in sperm by their innate antioxidant system consisting of enzymes, which include superoxide dismutase (SOD). This study aimed to assess the quality of semen from Itik Pinas-Khaki (IP-Khaki) drakes that were cryopreserved with either 4.5% DMSO or 7.0% glycerol as cryoprotectant through evaluation of total sperm motility (%) and determination of SOD activity (U/mL). Here, semen samples were collected from 12 sexually mature IP-Khaki drakes, an improved egg-type breed of Philippine mallard duck, and processed using modified reported cryopreservation procedure for ducks. Results showed that post-thawing total sperm motility averages of 12.04±5.61% using 4.5% DMSO and 13.99±5.28% using 7.0% glycerol were comparable. Moreover, similar SOD activity levels of 0.39±0.18 U/mL with 4.5% DMSO and 0.33±0.21 U/mL with 7.0% glycerol in 2.00 x 108 IP-Khaki sperm cells were also observed. The observed very low intracellular SOD activity indicates severe damage to sperm cells due to cryopreservation, which resulted in a comparably low total sperm motility with either of the cryoprotectants. Thus, the cryopreservation protocol used is not the optimum for IP-Khaki semen based on the observed considerable decline in sperm motility and very low SOD activity after cryopreservation.

Effects of different concentrations of honeysuckle (Lonicera iberica M. Bieb.) and barberry (Berberis vulgaris L.) extracts in cryopreserving of duck semen on sperm quality

“…In our study, the ejaculate volume was determined to be 0.68 ± 0.50 ml (Table 2). It was higher than the value given by ANCUELO et al (2021) andCYRIAC et al (2013), but within the range given by WAGNER and PINGEL (1995). These differences could be related to factors such as frequency of ejaculate collection, age, season and feeding.…”

“…Other studies in ducks have found sperm concentrations of 1.5-8.0 x109 sperm/ml (ANCUELO et al, 2021), 3.03 x109 sperm/ml (CYRIAC et al, 2013) and 0.84-1.3x109 sperm/ml (NAHAK et al, 2015), respectively. The collection method, the frequency of collection, the duck species -all these factors can influence sperm concentration.…”

“…The ejaculate volume was reported to be 0.14 ± 0.09 ml in the study of ANCUELO et al (2021) on Anas platyrhynchos and 0.18 ml in the study of CYRIAC et al (2013) on native ducks from India. Similarly, WAGNER and PINGEL (1995) found a volume of 0.10-0.70 ml in Peking ducks.…”

Addition of soybean meal extract with Lactobacillus plantarum in rations on protein digestibility and performance of broiler chickens

“…SOD is an antioxidant enzyme that is the first and main line against oxidative damage (Kismiati et al, 2021). Superoxide dismutase, which is produced by the liver, is vital in maintaining free radical levels in ROS form in normal settings, preventing an oxidation reaction that might harm the body (Ancuelo et al, 2021). Habashy et al (2019) stated that the SOD mechanism converts oxidative peroxide (H 2 O 2 ) into H 2 O and O 2 in order to protect cells from the oxidative damage caused by H 2 O 2 .…”

Avaliação mutagênica, genotóxica, carcinogênica e in silico da miotoxina BthTX-I isolada da peçonha da serpente Bothrops jararacussu

“…Os resultados são quantificados medindo a proteção produzida pelos antioxidantes(Cao et al 1993).Inúmeros trabalhos têm utilizado o ORAC para avaliar a capacidade antioxidante total de diferentes amostras(Abraham et al 2021, Ivy et al 2021, Quek et al 2021).4.3. Ensaio da superóxido dismutase SODOs sistemas de defesa intracelulares, que protegem as células de danos induzidos por espécies reativas de oxigênio (ROS), incluem enzimas como a superóxido dismutase (SOD), que catalisa alternadamente a dismutação do radical superóxido (O2 ) em qualquer oxigênio molecular comum (O2) ou peróxido de hidrogênio (H2O2), Ancuelo et al 2021.A metodologia in silico de identificação de moléculas principais contra uma proteína alvo tem sido amplamente utilizada na descoberta de novas abordagensterapêuticas (Singh et al 2021; Hajji et al 2022). da Silva, C.P., et al, 2015.…”