Superoxide released into the mitochondrial matrix

Oxidized Metabolites of Oltipraz Exert Cytoprotective Effects against Arachidonic Acid through AMP-Activated Protein Kinase-Dependent Cellular Antioxidant Effect and Mitochondrial Protection

“…The detailed mechanism remains to be investigated. In mitochondria, the electron transport chain complexes I and III are the main sites of superoxide radical production (Meany et al, 2006). AA also directly inhibits complex I and III in the respiratory chain of mitochondria (Cocco et al, 1999).…”

Recent advances in the analysis of biological particles by capillary electrophoresis

“…A general review covering the effort to develop techniques for the single-cell analysis of individual organelles has appeared [38]. The CE analysis of subcellular particles has included the analysis of new types of subcellular particles [7,39,40], separation conditions [41], improved detection arrangements [42,43], new applications [44][45][46] and new sampling and labeling schemes [47][48][49].…”

“…Recent reports have included the use of CE-LIF for the analysis of acidic organelles [40], drug content [39,46], mitochondrial DNA content [45] and superoxide production [44]. It is hypothesized that acidic organelles, including endosomes and lysosomes, play an important role in the cellular metabolism of chemotherapeutic drugs in certain forms of multidrug resistance.…”

“…Superoxide is involved in the oxidative damage of mitochondrial proteins, lipids and DNA, that is associated with the progression of aging. Meany et al [44] developed a CE-LIF method for measuring the superoxide released within mitochondria in the human osteosarcoma 143B cell line (see Fig. 2).…”

Increased ROS generation and SOD activity in heteroplasmic tissues of transmitochondrial mice with A3243G mitochondrial DNA mutation

“…The respiratory chain complexes were assessed as described previously (Lionetti et al, 2004;Panov et al, 2005;Bonora et al, 2006;Meany et al, 2006;Long et al, 2007). For the assessment of ROS production, tissue samples were washed with PBS, digested with 0.5% trypsin and 0.1% collagenase for 40 min, centrifuged at 600 rpm for 5 min and resuspended in D-Hank's buffer.…”