Superparamagnetic iron-oxide nanoparticles mPEG350– and mPEG2000-coated: cell uptake and biocompatibility evaluation

Silva, Adny Henrique; Lima, Enio; Mansilla, M. Vásquez; Zysler, R.D.; Troiani, Horacio Esteban; Mojica-Pisciotti, Mary Luz; Locatelli, Claudriana; Benech, Juan Claudio; Oddone, Natalia; Zoldan, Vinícius C.; Winter, Evelyn; Pasa, André A.; Creczynski-Pasa, Tânia B.

doi:10.1016/j.nano.2015.12.371

Cited by 57 publications

(39 citation statements)

References 51 publications

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“…The uptake kinetics of various SPIONs (DMSAcoated, PEGylated, pullulan coated, Dextran coated) have previously been studied in a variety of cell lines (MCF-7, MDCK, NIH-3 T3, CHO-K1, Brain Capillary Endothelial Cells (BCEC), human Blood Outgrowth Endothelial Cells (hBOEC)), measured through a host of techniques including magnetization measurements, Prussian blue staining, AFM, TEM and fluorescence imaging (Soenen et al 2010;Thomsen et al 2013;Calero et al 2015;Hanot et al 2015;Silva et al 2016). However, previous studies did not made use of label-free imaging, instead utilizing fluorescent imaging (Soenen et al 2010;Thomsen et al 2013;Calero et al 2015;Silva et al 2016). NP labeling encompasses a host of potential problems, including loss of label following uptake, difficulty in label attachment, modification of NP surface properties in addition to phototoxicity and photobleaching effects in cells.…”

Section: Discussionmentioning

confidence: 99%

Mechanisms for cellular uptake of nanosized clinical MRI contrast agents

2020

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Engineered Nanomaterials (NMs), such as Superparamagnetic Iron Oxide Nanoparticles (SPIONs), offer significant benefits in a wide range of applications, including cancer diagnostic and therapeutic strategies. However, the use of NMs in biomedicine raises safety concerns due to lack of knowledge on possible biological interactions and effects. The initial basis for using SPIONs as biomedical MRI contrast enhancement agents was the idea that they are selectively taken up by macrophage cells, and not by the surrounding cancer cells. To investigate this claim, we analyzed the uptake of SPIONs into well-established cancer cell models and benchmarked this against a common macrophage cell model. In combination with fluorescent labeling of compartments and siRNA silencing of various proteins involved in common endocytic pathways, the mechanisms of internalization of SPIONs in these cell types has been ascertained utilizing reflectance confocal microscopy. Caveolar mediated endocytosis and macropinocytosis are both implicated in SPION uptake into cancer cells, whereas in macrophage cells, a clathrin-dependant route appears to predominate. Colocalization studies confirmed the eventual fate of SPIONs as accumulation in the degradative lysosomes. Dissolution of the SPIONs within the lysosomal environment has also been determined, allowing a fuller understanding of the cellular interactions, uptake, trafficking and effects of SPIONs within a variety of cancer cells and macrophages. Overall, the behavior of SPIONS in non-phagocytotic cell lines is broadly similar to that in the specialist macrophage cells, although some differences in the uptake patterns are apparent. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Mechanisms for cellular uptake of nanosized clinical MRI contrast agents

2020

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“…methodologies. Rare studies performed in cellulo have shown that nanoparticles' properties (e.g., coating, size) influence their transformations (26)(27)(28). However, the cellular factors that influence the lysosomal degradation still need to be explored.…”

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confidence: 99%

Biosynthesis of magnetic nanoparticles from nano-degradation products revealed in human stem cells

Walle

Sangnier

Abou‐Hassan

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

111

120

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While magnetic nanoparticles offer exciting possibilities for stem cell imaging or tissue bioengineering, their long-term intracellular fate remains to be fully documented. Besides, it appears that magnetic nanoparticles can occur naturally in human cells, but their origin and potentially endogenous synthesis still need further understanding. In an effort to explore the life cycle of magnetic nanoparticles, we investigated their transformations upon internalization in mesenchymal stem cells and as a function of the cells’ differentiation status (undifferentiated, or undergoing adipogenesis, osteogenesis, and chondrogenesis). Using magnetism as a fingerprint of the transformation process, we evidenced an important degradation of the nanoparticles during chondrogenesis. For the other pathways, stem cells were remarkably “remagnetized” after degradation of nanoparticles. This remagnetization phenomenon is the direct demonstration of a possible neosynthesis of magnetic nanoparticlesin celluloand could lay some foundation to understand the presence of magnetic crystals in human cells. The neosynthesis was shown to take place within the endosomes and to involve the H-subunit of ferritin. Moreover, it appeared to be the key process to avoid long-term cytotoxicity (impact on differentiation) related to high doses of magnetic nanoparticles within stem cells.

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“…It should be noted that the degradation of polymer-coated magnetic particles is necessary because cytotoxicity and poor transgene expression can occur [170,171]. However, it has been suggested that the loading of magnetic nanoparticles into a biodegradable lipid or polymer can prevent these consequences [172,173,174].…”

Section: Triggers For Gene Release In Cancer Cells Using Gene Tranmentioning

confidence: 99%

Trigger-Responsive Gene Transporters for Anticancer Therapy

et al. 2017

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In the current era of gene delivery, trigger-responsive nanoparticles for the delivery of exogenous nucleic acids, such as plasmid DNA (pDNA), mRNA, siRNAs, and miRNAs, to cancer cells have attracted considerable interest. The cationic gene transporters commonly used are typically in the form of polyplexes, lipoplexes or mixtures of both, and their gene transfer efficiency in cancer cells depends on several factors, such as cell binding, intracellular trafficking, buffering capacity for endosomal escape, DNA unpacking, nuclear transportation, cell viability, and DNA protection against nucleases. Some of these factors influence other factors adversely, and therefore, it is of critical importance that these factors are balanced. Recently, with the advancements in contemporary tools and techniques, trigger-responsive nanoparticles with the potential to overcome their intrinsic drawbacks have been developed. This review summarizes the mechanisms and limitations of cationic gene transporters. In addition, it covers various triggers, such as light, enzymes, magnetic fields, and ultrasound (US), used to enhance the gene transfer efficiency of trigger-responsive gene transporters in cancer cells. Furthermore, the challenges associated with and future directions in developing trigger-responsive gene transporters for anticancer therapy are discussed briefly.

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Superparamagnetic iron-oxide nanoparticles mPEG350– and mPEG2000-coated: cell uptake and biocompatibility evaluation

Cited by 57 publications

References 51 publications

Mechanisms for cellular uptake of nanosized clinical MRI contrast agents

Mechanisms for cellular uptake of nanosized clinical MRI contrast agents

Biosynthesis of magnetic nanoparticles from nano-degradation products revealed in human stem cells

Trigger-Responsive Gene Transporters for Anticancer Therapy

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