Superparamagnetic Nanoparticles as a Powerful Systems Biology Characterization Tool in the Physiological Context

Salaklang, Jatuporn; Steitz, Benedikt; Finka, Andrija; O’Neil, Conlin P.; Moniatte, Marc; Vlies, André J. van der; Giorgio, Todd D.; Hofmann, Heinrich; Hubbell, Jeffrey A.; Petri‐Fink, Alke

doi:10.1002/anie.200800357

Cited by 39 publications

(37 citation statements)

References 12 publications

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“…This is possible given that Hsp90 is involved in the import of mitochondrial proteins, the most abundant of which being Hsp60 and mortalin (mtHsp70). In mammalian cells, the cytosolic Hsp70 and Hsp90 can bind the Tom70 and Tom34 that are outer components of the mitochondrial import pore and facilitate the transfer of a set of preproteins to the receptor for ensuing translocation across the mitochondrial membrane (Young et al 2003;Cassina and Casari 2009;Salaklang et al 2008). The proteomic data suggests that at any given moment, at most 3 % of the cytosolic Hsp90 can be recruited to the translocation of polypeptides to the mitochondria.…”

Section: Proteinmentioning

confidence: 99%

Proteomic data from human cell cultures refine mechanisms of chaperone-mediated protein homeostasis

Finka

Goloubinoff

2013

Cell Stress and Chaperones

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192

212

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In the crowded environment of human cells, folding of nascent polypeptides and refolding of stressunfolded proteins is error prone. Accumulation of cytotoxic misfolded and aggregated species may cause cell death, tissue loss, degenerative conformational diseases, and aging. Nevertheless, young cells effectively express a network of molecular chaperones and folding enzymes, termed here "the chaperome," which can prevent formation of potentially harmful misfolded protein conformers and use the energy of adenosine triphosphate (ATP) to rehabilitate already formed toxic aggregates into native functional proteins. In an attempt to extend knowledge of chaperome mechanisms in cellular proteostasis, we performed a meta-analysis of human chaperome using high-throughput proteomic data from 11 immortalized human cell lines. Chaperome polypeptides were about 10 % of total protein mass of human cells, half of which were Hsp90s and Hsp70s. Knowledge of cellular concentrations and ratios among chaperome polypeptides provided a novel basis to understand mechanisms by which the Hsp60, Hsp70, Hsp90, and small heat shock proteins (HSPs), in collaboration with cochaperones and folding enzymes, assist de novo protein folding, import polypeptides into organelles, unfold stress-destabilized toxic conformers, and control the conformal activity of native proteins in the crowded environment of the cell. Proteomic data also provided means to distinguish between stable components of chaperone core machineries and dynamic regulatory cochaperones.

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Section: Proteinmentioning

confidence: 99%

Proteomic data from human cell cultures refine mechanisms of chaperone-mediated protein homeostasis

Finka

Goloubinoff

2013

Cell Stress and Chaperones

Self Cite

192

212

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“…Salaklang et al [289] used cyclic RGD (cRGD, containing the Arg-Gly-Asp motif) in parallel with fluorescently labeled mitochondrial targeting aminopropyltriethoxysilane-coated SPIONs in order to target and isolate mitochondria. The cyclic pentapeptide cRGD was used in order to enhance the receptor-specific uptake of nanoparticles [290].…”

Section: Biological Response Of Spionsmentioning

confidence: 99%

Recent advances in surface engineering of superparamagnetic iron oxide nanoparticles for biomedical applications

Mahmoudi

Simchi

Imani

2010

JICS

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Superparamagnetic iron oxide nanoparticles (SPIONs) are promising materials for various biomedical applications including targeted drug delivery and imaging, hyperthermia, magneto-transfections, gene therapy, stem cell tracking, molecular/cellular tracking, magnetic separation technologies (e.g. rapid DNA sequencing), and detection of liver and lymph node metastases. The most recent applications for SPIONs for early detection of inflammatory, cancer, diabetes and atherosclerosis have also increased their popularity in academia. In order to increase the efficacy of SPIONs in the desired applications, especial surface coating/characteristics are required. The aim of this article is to review the surface properties of magnetic nanoparticles upon synthesis and the surface engineering by different coatings. The biological aspects, cytotoxicity, and health risks are addressed. Special emphasis is given to organic and inorganic-based coatings due to their determinant role in biocompatibility or toxicity of the final particles.

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“…2,3 Surface functionalization strategies have been established during recent years using standard bioconjugation techniques 4 , supramolecular or bioorthogonal chemistry 5 , or nanoparticles (NPs) such as gold 6 , which are almost readymade for coupling procedures due to their particular chemistry. Despite significant progress in the field, surface functionalization is usually achieved in a tedious process by adapting typical synthesis parameters such as reaction time, concentration or pH.…”

Section: Introductionmentioning

confidence: 99%

Magnetic microreactors for efficient and reliable magnetic nanoparticle surface functionalization

et al. 2014

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Microreactors have attracted wide attention in the nano-and biotechnology field because they offer many advantages over standard liquid phase reactions. We report the development of a magnetic microreactor for reliable, fast and efficient surface functionalization of superparamagnetic iron oxide nanoparticles (SPIONs). A comprehensive study is described of the development process in terms of setup, loading capacity and efficiency. We have performed experimental and computational studies in order to evaluate the trapping efficiencies, maximum loading capacity and magnetic alignment of the nanoparticles. The results show that capacity and trapping efficiencies are directly related to the flow rate, elution time and reactor type. Based on our results and the developed magnetic microreactor, we describe a model multistep surface derivatization procedure of SPIONs.

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Superparamagnetic Nanoparticles as a Powerful Systems Biology Characterization Tool in the Physiological Context

Cited by 39 publications

References 12 publications

Proteomic data from human cell cultures refine mechanisms of chaperone-mediated protein homeostasis

Proteomic data from human cell cultures refine mechanisms of chaperone-mediated protein homeostasis

Recent advances in surface engineering of superparamagnetic iron oxide nanoparticles for biomedical applications

Magnetic microreactors for efficient and reliable magnetic nanoparticle surface functionalization

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