Superparamagnetic Nanoparticles Based Immunomagnetic Separation—Multiplex Polymerase Chain Reaction Assay for Detection of <i>Salmonella</i>

Padmavathy, B.; Patel, Amee B.; Kumar, R. Vinoth; Ali, B. M. Jaffar

doi:10.1166/sam.2012.1259

Cited by 8 publications

(3 citation statements)

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“…To date, metal nanoparticles with diverse shapes and sizes have been prepared with wet chemistry approaches. The unique structural and functional features of metal nanoparticles make them excellent candidates for the development of electrochemical catalysts, electrical immunosensors, and supports for enzyme immobilization . These types of immobilized enzymes have shown higher activity and thermal stability than their free counterparts .…”

Section: Nanomaterials Scaffolds For Biomass Conversion and Immobilizamentioning

confidence: 99%

Design of nanoscale enzyme complexes based on various scaffolding materials for biomass conversion and immobilization

Hyeon

Shin

Han

2016

Biotechnology Journal

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The utilization of scaffolds for enzyme immobilization involves advanced bionanotechnology applications in biorefinery fields, which can be achieved by optimizing the function of various enzymes. This review presents various current scaffolding techniques based on proteins, microbes and nanomaterials for enzyme immobilization, as well as the impact of these techniques on the biorefinery of lignocellulosic materials. Among them, architectural scaffolds have applied to useful strategies for protein engineering to improve the performance of immobilized enzymes in several industrial and research fields. In complexed enzyme systems that have critical roles in carbon metabolism, scaffolding proteins assemble different proteins in relatively durable configurations and facilitate collaborative protein interactions and functions. Additionally, a microbial strain, combined with designer enzyme complexes, can be applied to the immobilizing scaffold because the in vivo immobilizing technique has several benefits in enzymatic reaction systems related to both synthetic biology and metabolic engineering. Furthermore, with the advent of nanotechnology, nanomaterials possessing ideal physicochemical characteristics, such as mass transfer resistance, specific surface area and efficient enzyme loading, can be applied as novel and interesting scaffolds for enzyme immobilization. Intelligent application of various scaffolds to couple with nanoscale engineering tools and metabolic engineering technology may offer particular benefits in research.

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Section: Nanomaterials Scaffolds For Biomass Conversion and Immobilizamentioning

confidence: 99%

Design of nanoscale enzyme complexes based on various scaffolding materials for biomass conversion and immobilization

Hyeon

Shin

Han

2016

Biotechnology Journal

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“…The high affinity and specificity of antibodies could be used as an alternative to microbiological plating methods by coupling them to solid phases (immunoaffinity chromatography) or surfaces of magnetic nanoparticles (immunomagnetic nanoparticles, IMPs). ,, Among other reasons, the use of animals during antibody manufacturing leads to relatively high production costs, volatile reproducibility, and physiological restrictions. Furthermore, directive 2010/63/EU limits the usage of animals for scientific purposes …”

Section: Introductionmentioning

confidence: 99%

Food Sensing: Selection and Characterization of DNA Aptamers to Alicyclobacillus Spores for Trapping and Detection from Orange Juice

Hünniger

Fischer

Wessels

et al. 2015

J. Agric. Food Chem.

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The quality of the beverage industry's products has to be constantly monitored to fulfill consumers' high expectations. The thermo-acidophilic Gram-positive Alicyclobacillus spp. are not pathogenic, but their heat-resistant endospores can survive juice-processing conditions and have become a major economic concern for the fruit juice industry. Current detection methods rely on cultivation, isolation, and organism identification, which can take up to a week, resulting in economic loss. This work presents the selection and identification of DNA aptamers targeting Alicyclobacillus spores by spore-SELEX (systematic evolution of ligands by exponential enrichment) in orange-juice-simulating buffer. The selection process was verified by various techniques, including flow cytometric binding assays, radioactive binding assays, and agarose gel electrophoresis. The subsequent aptamer characterization included the determination of dissociations constants and selectivity by different techniques, such as surface plasmon resonance spectroscopy and fluorescence microscopy. In summary, 10 different aptamers with an affinity to Alicyclobacillus spp. have been developed, analyzed, and characterized in terms of affinity and specificity.

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“…Immunomagnetic separation has been applied for rapid detection for pathogens, making it possible to separate and enrich the target bacteria rapidly using a magnetic field. Padmavathy et al (2012) successfully prepared anti-Salmonella polysera, and showed rapid separation and detection of this organism. However, preparing antibodies for Cronobacter spp.…”

Section: Introductionmentioning

confidence: 99%

Detection of Cronobacter species in powdered infant formula by probe-magnetic separation PCR

Ming

et al. 2014

Journal of Dairy Science

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Cronobacter species are opportunistic foodborne pathogens associated with serious infections in preterm neonates and infants. Based on the epidemiological research, infant formula products are considered to be the main source of infections from this organism. Therefore, accurate methods are required for detection of Cronobacter species. In this study, the specific probe and primers for detection of this organism were designed and verified. The probe-magnetic beads were prepared for sequence capture, followed by PCR assay to detect the target gene. This probe-magnetic separation PCR assay could detect as few as 10(3) cfu/mL of Cronobacter in artificially contaminated infant formulas in less than 4 h. The combination of magnetic beads and PCR showed the potential for the detection of Cronobacter in infant formulas and may have applications in the dairy industry.

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Superparamagnetic Nanoparticles Based Immunomagnetic Separation—Multiplex Polymerase Chain Reaction Assay for Detection of Salmonella

Cited by 8 publications

References 0 publications

Design of nanoscale enzyme complexes based on various scaffolding materials for biomass conversion and immobilization

Design of nanoscale enzyme complexes based on various scaffolding materials for biomass conversion and immobilization

Food Sensing: Selection and Characterization of DNA Aptamers to Alicyclobacillus Spores for Trapping and Detection from Orange Juice

Detection of Cronobacter species in powdered infant formula by probe-magnetic separation PCR

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