Suplementação de vitamina C não acelera o processo de consolidação de fratura da tíbia em ratos

Abstract

ObjectiveTo investigate the role of ascorbic acid supplementation on bone healing after rat tibia fracture.MethodsThirty male Wistar rats were randomly divided into Vitamin C (Group A) and sham (Group B) groups (15 rats each). Group A received 200 mg intraperitoneally per kg per day of ascorbic acid and Group B was given saline 5 ml per kg per day intraperitoneally once a day. The animals were caged in pairs and allowed free access to tap water and a standard rodent chow ad libitum. Fractures were produced manually, they were not stabilized, and unprotected weight-bearing was allowed. At two, four, and six weeks post-fracture, the rats in both groups were anesthetized and sacrificed by cervical dislocation. Callus tissue was dissected, prepared, and analyzed histologically. Histomorphological analysis was performed at six weeks post-fracture and the extent of fracture healing was determined using a five-point scale.ResultsThere were no histological and histomorphological differences between drug-treated animals and the sham in the three different stages studied. By six weeks post-fracture, the five animals of each group had a complete bone union.ConclusionUnder the studied conditions, intraperitoneal Vitamin C supplementation does not accelerate the fracture healing process after experimental tibia fracture in rats. Level of evidence: Level 2, individual study with experimental design.

Sequential Analysis of Oxidative Stress Markers and Vitamin C Status in Acute Bacterial Osteomyelitis

“…Evidences suggest that Asc can ameliorate xenobiotic-induced toxicity at systemic levels [ 33 ] as well as the bone tissue [ 36 ]. Studies on fracture healing were less conclusive, and although Asc supplementation improved the mechanical resistance of fracture callus in elderly vitamin C genetically deficient rats, no histological or clinical improvements were seen in wild-type animals [ 37 , 38 ].…”

Multifactorial Effect of Ascorbic Acid on the Process of Reparative Osteogenesis

“…Установлено, что внутрибрюшинное введение аскорбата (200 мг/кг в сутки) в течение 3 суток до и трижды в неделю в течение 21 суток после моделирования перелома трубчатой кости у крыс ускоряет заживление; однако его сочетанное применение с витамином Е в те же сроки снижает стимулирующий эффект [36]. Внутрибрюшинные инъекции тех же доз аскорбата только лишь в послеоперационном периоде не оказывают заметного влияния на сращение костных отломков [37]. Тем не менее, внутрибрюшинное введение 500 мг/кг витамина С в послеоперационном периоде предотвращает негативное влияние свободнорадикального окисления, индуцированного стимуляцией нейтрофилов инъекциями зимозана, на заживление переломов плечевой кости [38].…”

Roles for Taraxacum officinale and Vitamins (C, K) on Bone Formation and Resorption Heparin-Induced in Rats

“…Group IV: Rats will be co-administered orally with vitamin K (50 mg/kg) [21] and vitamin C (200 mg/ kg) [22] with heparin injection for 30 days.…”