Supplement 2002 (no. 46) to the Kauffmann–White scheme

Popoff, M.Y.; Bockemühl, J; Gheesling, Linda L.

doi:10.1016/j.resmic.2004.04.005

Cited by 231 publications

(136 citation statements)

References 2 publications

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“…In humans, it is considered one of the most important etiological agents of foodborne disease. [1][2][3] Of all the foods that may transmit the pathogen to man, poultry products, eggs and poultry carcasses are those that play the most important roles. 4 In the last 40 years, there have been thousands of references in the scientific literature connecting Salmonella to birds, 5 to poultry products, eggs and poultry carcasses as being responsible for several outbreaks.…”

Section: Introductionmentioning

confidence: 99%

Salmonella spp. in poultry carcass: evaluation of sample preparation methods and effect of storage under refrigeration on pathogen recovery

Yamatogi

Padovani

Galvão

et al. 2012

Microbiol Res (Pavia)

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The aim of the present study was to evaluate the use of different analytical units and the influence of storage under refrigeration on the detection of Salmonella sp. in naturally contaminated poultry carcasses. One hundred and thirty samples were collected during the production process soon after chilling (postchiller phase). Fifty-five samples were analyzed in up to 2 h after collection and 65 samples were analyzed after 72 h of storage. Pathogen screening was based on three different analytical units and a comparison was made between them. Carcasses were initially rinsed with 400 mL of diluent, and three different analytical units were incubated: total rinsing volume (TRV), a single 30 mL aliquot of the rinsing volume, and 25 g of skin from different areas of the carcass. Of all samples analyzed, 60% were positive for Salmonella sp. From the samples collected at the post-chiller phase, 57% were positive for the pathogen and 52.31% of these were detected by TRV; a better statistical performance (P<0.05) when compared to the other analytical units. Of the refrigerated samples, 63% were contaminated, but there were no significant differences between analytical units (P>0.05). There were no significant differences between the number of positive samples from the post-chiller phase and after 72 h of refrigeration. It was also seen that the use of different analytical units (one for the post-chiller phase and another for the refrigerated samples) in samples coming from the same production lot may give different results.

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Section: Introductionmentioning

confidence: 99%

Salmonella spp. in poultry carcass: evaluation of sample preparation methods and effect of storage under refrigeration on pathogen recovery

Yamatogi

Padovani

Galvão

et al. 2012

Microbiol Res (Pavia)

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“…Salmonella enterica serovar Typhimurium (S. Typhimurium), one of more than 2500 serovars recognized in the genus Salmonella (Popoff et al, 2004), is a major bacterial pathogen that causes food-borne infection (salmonellosis) in humans and animals worldwide. For many years it has been the most common serovar isolated from humans and animals in Australia.…”

Section: Introductionmentioning

confidence: 99%

Extended phage locus typing of Salmonella enterica serovar Typhimurium, using multiplex PCR-based reverse line blot hybridization

Wang

Kong

Jelfs

et al. 2008

Journal of Medical Microbiology

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Salmonella enterica serovar Typhimurium (S. Typhimurium) is the commonest pathogen causing food-borne disease among humans and animals in Australia. A multiplex PCR-based reverse line blot (mPCR/RLB) system was developed to rapidly identify S. Typhimurium phage types and strains within them. The system comprised 32 biotin-labelled primer sets and 38 amino-labelled probes, based on sequences that were either phage-type-related or derived from temperate phages ST64B, P22, Gifsy-1 or Gifsy-2. The system was developed and evaluated using 168 S. Typhimurium isolates, representing 46 phage types. RLB patterns, based on a combination of positive hybridization and grading of signal intensities, validated by sequencing, differentiated S. Typhimurium isolates into 102 types. Some clusters contained isolates belonging to a single phage type while others contained isolates belonging to more than one. Most phage types exhibited at least two RLB profiles. The feasibility of this system was evaluated during investigations of three outbreaks, due to two different phage types. Within each outbreak, isolates showed identical RLB patterns, whereas sporadic isolates of corresponding phage types showed various patterns. The mPCR/RLB system was compared with multilocus variable-number tandem-repeat analysis (MLVA). The two methods demonstrated similar discriminatory abilities. Based on these preliminary results, the mPCR/RLB system is a promising tool for molecular identification of most common S. Typhimurium phage types. It could be used as an alternative to, or in conjunction with, MLVA for rapid strain typing during outbreaks.

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“…The variability defined by immunologic characterization of two surface structures, O-polysaccharide (O antigen) and flagellin protein (H antigen), provides the basis for serotyping Salmonella spp. into serogroups [6]. Polymorphism in the bla TEM genes has already been noted for clinical isolates with > 150 variants [7].…”

Section: Introductionmentioning

confidence: 80%

Molecular characterization and antibiotic resistance of Salmonella in children with acute gastroenteritis in Abuja, Nigeria

Ifeanyi

Bassey

Ikeneche

et al. 2014

J Infect Dev Ctries

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Introduction: In Nigeria, acute gastroenteritis in children under five years of age is a major cause of mortality and morbidity; identification and characterization of microbial agents of acute gastroenteritis, including Salmonella, remains a powerful tool for effective management, surveillance, and control. Methodology: Diarrheal stool samples were directly plated onto differential and selective media to isolate Salmonella. Extended-spectrum beta-lactamases were screened using the double disk diffusion technique and by PCR targeting the bla TEM and bla CTX-M genes. Pulsed-field gel electrophoresis (PFGE) was performed usingthe PulseNet Canada Laboratory protocol for molecular subtyping using the restriction enzymes XbaI and BlnI. Results: The serotypes identified were Salmonella enterica serovar Zanzibar (n = 5), Salmonella Brancaster (n = 3), and one isolate of Salmonella Enteritidis (phage type 1). The following levels of resistance were found among the Salmonella strains: amoxicillin, five strains (55.6%); amoxicillin-clavulanic acid, two strains (22.2%); cephalexin, five strains (55.6%); and cefuroxime, five strains (55.6%). Intermediate resistance was found in five strains (55.6%) only to amoxicillin-clavulanic acid. All isolates were susceptible to nalidixic acid, ciprofloxacin, and ceftriaxone, and no ESBL-producing Salmonella were detected. Conclusions: Our findings demonstrated the involvement of three Salmonella serovars in acute gastroenteritis; resistance to penicillins and cephalosporins was common.

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Supplement 2002 (no. 46) to the Kauffmann–White scheme

Cited by 231 publications

References 2 publications

Salmonella spp. in poultry carcass: evaluation of sample preparation methods and effect of storage under refrigeration on pathogen recovery

Salmonella spp. in poultry carcass: evaluation of sample preparation methods and effect of storage under refrigeration on pathogen recovery

Extended phage locus typing of Salmonella enterica serovar Typhimurium, using multiplex PCR-based reverse line blot hybridization

Molecular characterization and antibiotic resistance of Salmonella in children with acute gastroenteritis in Abuja, Nigeria

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