Supplemental protease with phytase and xylanase and cereal grain source affected nutrient digestibility and performance of broilers

Abstract

A study was conducted to evaluate the effects of supplemental protease and cereal grain type on nutrient digestibility (jejunum and ileum) and performance of broilers offered diets with reduced amino acid concentrations and supplemental xylanase and phytase. A total of 624 male broiler chicks (Ross 308) were randomly distributed into 48 floor pens (13 chicks/pen; 0.07 m2/bird) and offered one of six dietary treatments with eight replicates per treatment. Dietary treatments were either maize- or wheat-based with a positive control (PC) reference diet, a negative control diet without protease (NC; 60 g/kg lower amino acid density than PC), and an NC diet with protease. The reduction in amino acid density affected (P<0.05) nutrient digestibility by varying degrees depending on cereal grain source. At 14 d of age, cereal grain and protease showed a significant interaction (P<0.05) which affected jejunal and ileal starch digestibility, whereby protease increased digestibility in birds fed wheat-based diets but not in those fed maize-based diets. Cereal grain source affected (P<0.05) nitrogen (jejunum and ileum) and digestible energy (DE; ileum), where birds fed wheat-based diets had higher digestibility than those fed maize-based diets. At 28 d of age, birds fed wheat-based diets had a higher (P<0.01) jejunal and ileal nitrogen digestibility, whereas protease reduced ileal nitrogen digestion. Protease affected ileal starch digestion in birds fed wheat, but not maize-based diets, resulting in a significant cereal grain × protease interaction (P<0.05). Wheat-based diets had a higher DE than maize-based diets in both the jejunum and ileum. From 15 to 35 d of age, cereal grain source (P<0.05) affected performance, whereby broilers offered maize-based diets had better performance than those fed wheat-based diets.

Growth performance and nutrient digestibility of broiler chickens as affected by a novel protease

“…2 ProAct360 ® , DSM Nutritional Products, Switzerland; 50 g/ton corn diluted premixes had 0, 17 or 50 of the protease corresponding to 0, 10,000 and 30,000 New Feed Protease (NFP) units. Vieira et al 10.3389/fanim.2022.1040051 effects (Kaczmarek et al, 2014;Yuan et al, 2017;Walk et al, 2019) to significant improvements in growth performance of broilers (Freitas et al, 2011;Lee et al, 2018;Jabbar et al, 2021;McCafferty et al, 2022). Synergy between proteases with other in feed supplemental enzymes, such as phytases, xylanases and amylases have also been reported (Cowieson and Adeola, 2005;Amerah et al, 2017;Cowieson et al, 2019).…”

“…Supplementation of proteases has lastly emerged as a tool to improve dietary protein utilization (Stefanello et al, 2016). Apart from animal performance, supplemental proteases have demonstrated to reduce feed costs as well as nitrogen excretion (Leinonen and Williams, 2015;McCafferty et al, 2022).…”

“…Research on the use of exogenous proteases has been ongoing for years and many commercially available products are currently utilized in poultry feeds. Reports on the effects of supplemental proteases have been related to benefits in performance (Ghazi et al, 2002;Angel et al, 2011;Moss et al, 2017;McCafferty et al, 2022), nutrient digestibility (Freitas et al, 2011;Liu et al, 2013;Stefanello et al, 2016), intestinal health (Xu et al, 2017;Cowieson et al, 2018), and immune competence of broilers (Peek et al, 2009;Cowieson et al, 2017).…”

Metabolizable Energy and Amino Acid Digestibility of Soybean Meal from Different Sources for Broiler Chickens Supplemented with Protease

“…As demonstrated in the present research, protease was effective in improving amino acid digestibility in all soybean meals. One explanation for these results is that proteolytic enzymes can catalyze the breakdown of peptide bonds found in feed proteins [ 25 ].…”