Supplementary Table 1, Supplementary Figures 1-8 from Centrosome Amplification in Cancer Disrupts Autophagy and Sensitizes to Autophagy Inhibition

Abstract

<p>Supp Table 1. Primer sequences for qRT-PCR. Supplemental Figure 1: Validation of centrosome amplification cell models. Supplemental Figure 2: Centrosome amplification induced by cytokinesis failure and STIL overexpression also disrupts autophagy. Supplemental Figure 3: PLK4 inhibition reduces centrosomes and autophagy defect in some cancer cell lines with centrosome amplification. Supplemental Figure 4: Centrosome amplification increases autophagy gene expression but does not significantly alter LC3B-II/I ratio. Supplemental Figure 5: Centrosome amplification does not alter lysosomal hub organization around the centrosomes. Supplemental Figure 6: Centrosome amplification-mediated autophagy disruption does not depend significantly on mitosis. Supplemental Figure 7: Microtubule disruption inhibits autophagy. Supplemental Figure 8: Centrosome amplified cells are less sensitive to mitotic inhibitors.</p>