Supplementation of Bacillus subtilis GM5 enhances broiler body weight gain and modulates cecal microbiota

Hadieva, Guzel Fanisovna; Lutfullin, Marat; Pudova, Daria; Akosah, Yaw; Shagimardanova, Elena; Gogoleva, Natalia; Шарипова, М. Р.; Марданова, А. М.

doi:10.1007/s13205-020-02634-2

Cited by 14 publications

(10 citation statements)

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“…They showed consistency with the findings of Bai et al (2017) , who tested the inclusion of Bacillus subtilis and found an increase in broiler growth performance, providing beneficial effects on broiler growth and potentially replacing antibiotic growth promoters ( Park et al, 2020 ). In broiler chickens, some certain strains of Bacillus subtilis such as Bacillus subtilis B10 ( Qin et al, 2018 ), Bacillus subtilis 29784 ( Jacquier et al, 2019 ), Bacillus subtilis DSM 32315 ( Bortoluzzi et al, 2018 ), Bacillus subtilis BYS2 ( Dong et al, 2020 ), Bacillus subtilis GM5 ( Hadieva et al, 2021 ), and Bacillus subtilis PB6 ( Abramowicz et al, 2019 ) have been suggested to promote growth activities and healthy GM. The expression of mRNA GHR and mRNA IGF-1 were increased in the BS -ATCC19659 (BS-1 and BS-3) groups in the liver.…”

Section: Discussionmentioning

confidence: 99%

Feeding Bacillus subtilis ATCC19659 to Broiler Chickens Enhances Growth Performance and Immune Function by Modulating Intestinal Morphology and Cecum Microbiota

et al. 2022

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This study investigated dietary supplementation with Bacillus subtilis (BS) ATCC19659 on growth performance, biochemical indices, intestinal morphology, and cecum microflora in broiler chicks. A total of 600 Arbor 1-day Acres broilers of either sex were allotted to 5 treatments: chicks were fed a corn- and soybean-based diet (CON); chicks were fed basal diet containing 500 mg ZnB/kg (ZnB); chicks were fed basal diet containing 1 × 108 CFU/g feed of BS-ATCC19659 (BS-1); chicks were fed basal diet containing 3 × 108 CFU/g feed of BS-ATCC19659 (BS-3); and chicks were fed basal diet containing 5 × 108 CFU/g feed of BS-ATCC19659 (BS-5). Each treatment comprised 6 replicates with 20 birds for each replicate pen. Chicks in the BS-5 and BS-3 groups had higher body weight at the 21st and 42nd days and average daily gain from 1 to 21 days than that in the CON group (p < 0.05). Chicks in the BS-5 and ZnB groups had higher serum antioxidant activities and immunity response than those in the CON group (p < 0.05). Compared with the CON group, the liver mRNA abundance of GHR, TGF-β, IGF-1, IFN-γ, SOD, CAT, and GPX of chicks in three BS groups and the ileum villus length (μm) of chicks in BS-3 and ZnB groups was increased (p < 0.05). Compared with the CON group, the villus height-to-crypt depth ratio of the ileum of chicks in the BS-5 and BS-3 groups and the crypt depth and villus height-to-crypt depth ratio of the jejunum in the BS-5 and ZnB groups were increased (p < 0.05). The abundance of the Cyanobacteria phyla in the cecum decreased in response to treatment with both BS-ATCC19659 and ZnB groups (p < 0.05). Compared with the CON group, the cecum abundance of genera GCA-900066575 (Lachnospiraceae), Anaerofustis, and Papillibacter (Firmicutes phylum) in three BS groups were increased (p < 0.05); The abundance of genus Escherichia–Shigella reduced in the BS-3 group (p < 0.05). Compared with the CON group, the cecum abundance of genus Clostridia_unclassified in ZnB and BS-5 groups was decreased (p < 0.05) of broilers. Generally, Bacillus subtilis ATCC19659 as feed additive positively affected growth performance, immunity response, and cecal microflora of broilers.

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Section: Discussionmentioning

confidence: 99%

Feeding Bacillus subtilis ATCC19659 to Broiler Chickens Enhances Growth Performance and Immune Function by Modulating Intestinal Morphology and Cecum Microbiota

et al. 2022

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“…In broiler chickens, B. subtilis GM5 was shown to improve feed consumption and digestibility, which is important for reducing production costs [ 19 ]. The probiotic supplement affected the meat quality of the experimental poultry.…”

Section: Discussionmentioning

confidence: 99%

Effect of a probiotic containing lactobacilli and bifidobacteria on the metabolic processes, litter microbiocenosis, and production indicators of broiler Pekin ducklings

et al. 2022

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Background and Aim: The use of antibiotics is prohibited in poultry feeding in many countries worldwide, which has resulted in the emergence of antibiotic-resistant bacteria. Therefore, probiotic supplements are a good alternative in these circumstances. Probiotics, including Bifidobacterium adolescentis and Lactobacillus acidophilus, can reduce the development of resistance and stimulate the growth of broiler ducklings. This study aimed to evaluate properties of the probiotic supplement containing these bacteria. Materials and Methods: We studied the response of broiler ducklings to the addition of a dietary probiotic supplement containing lactobacilli and bifidobacteria until they reached 7 weeks of age. All birds participating in the experiment received probiotic supplements on days 1-7, 15-21, 29-35. The state of the ducklings was assessed on day 21. At the age of 21 and 42 days, 4-5 ml of blood was drawn from the wing vein of 10 randomly selected birds (5 in each group). Blood samples were analyzed for total protein, concentration of glucose, hemoglobin, calcium and inorganic phosphorus, as well as the number of erythrocytes and leukocytes. Enzymatic calorimetric method, molybdate method, and haemocytometry according to Nutt method and haemoglobin cyanide method were used to analyze blood samples. Results: The live weight of the experimental ducklings increased by 5.0%, showing a positive effect of probiotic supplementation, whereas their feed consumption per kilogram of weight gain decreased. Their hemoglobin content and red blood cell count increased. Although the number of lactobacilli and bifidobacteria increased, the number of Escherichia coli cells decreased 2.15-fold (p<0.05). The addition of the probiotic supplement contributed to improving the digestibility of protein and fat by 1.6%, fiber by 3.4%, and nitrogen-free extractive substances by 4.7%. The broiler ducklings had high meat quality indicators, including dressing percentage, which increased by 5.4%, whereas the fat content decreased by 1.3%. Conclusion: The introduction of the probiotic supplement in the diet of broiler ducklings improved their growth indicators and increased the number of lactobacilli and bifidobacteria while decreasing the number of E. coli cells in the intestine. It not only improved the meat quality but also increased the profit from $0.392 per bird in the control group to $0.472 per bird in the experimental group. Therefore, this probiotic supplement is a good alternative for raising ducklings in large enterprises and farms. The study limitations may be that the results are only applicable to broiler ducklings. The use of lactobacilli may yield different results in other bird breeds or broiler ducklings in different age groups.

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“…Bacillus subtilis is known for its beneficial effects by producing antimicrobial agents and surfactants ( 9, 10 ), by reducing intestinal inflammation ( 9, 11, 12 ), and by modulating or restoring the gut microbiota ( 13, 14 ), despite its lower abundance in the microbial communities. Here we focused on the combination of B. subtilis and E. faecalis and on their relationship when B. subtilis is a minor population, such as an additive.…”

Section: Introductionmentioning

confidence: 99%

“…Since E. faecalis is present in communities with other bacteria in the above situations, investigating how E. faecalis is associated with other bacteria is the key to establishing a way to control E. faecalis. Bacillus subtilis is known for its beneficial effects by producing antimicrobial agents and surfactants (9,10), by reducing intestinal inflammation (9,11,12), and by modulating or restoring the gut microbiota (13,14), despite its lower abundance in the microbial communities. Here we focused on the combination of B. subtilis and E. faecalis and on their relationship when B. subtilis is a minor population, such as an additive.…”

Section: Introductionmentioning

confidence: 99%

Bacillus subtilis with a 1% population enhances the activity of Enterococcus faecalis with a 99% population

Ito

Yamanashi

2021

Preprint

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Microbes are present as communities in the environment. However, the importance of minor populations has not been well studied experimentally. In this study, we evaluated the role of Bacillus subtilis with a 1% population and its effect on co-incubated Enterococcus faecalis with a 99% population. Here we used an azo dye-decolorizing Enterococcus faecalis strain T6a1 and non-dye-decolorizing Bacillus subtilis strain S4ga. The dye decolorization assay enabled the investigation of the effects of Bacillus subtilis S4ga on the activity of Enterococcus faecalis T6a1, even when Bacillus subtilis S4ga was present at only 1% relative abundance or lower. We found that non-decolorizing Bacillus subtilis S4ga enhanced the dye decolorization activity of Enterococcus faecalis T6a1, shortened the lag time of Enterococcus faecalis T6a1 to start decreasing the dye concentration, and increased the time for Enterococcus faecalis T6a1 to continue dye decolorization. These effects were correlated with redox potential values. We compared the extracellular amino acids between each incubation culture of Enterococcus faecalis T6a1 and Bacillus subtilis S4ga, which revealed their mutual relationship by cross-feeding of specific amino acids. We also compared the intracellular primary metabolites between co-incubation and sole incubation of E. faecalis T6a1. The arginine deiminase (ADI) pathway in the co-incubated E. faecalis T6a1 was activated compared to that of E. faecalis T6a1 incubated solely. These findings explained that co-incubation with Bacillus subtilis S4ga promoted ATP production in Enterococcus faecalis T6a1 cells to a greater extent and enhanced dye-decolorization activity.

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Supplementation of Bacillus subtilis GM5 enhances broiler body weight gain and modulates cecal microbiota

Cited by 14 publications

References 59 publications

Feeding Bacillus subtilis ATCC19659 to Broiler Chickens Enhances Growth Performance and Immune Function by Modulating Intestinal Morphology and Cecum Microbiota

Feeding Bacillus subtilis ATCC19659 to Broiler Chickens Enhances Growth Performance and Immune Function by Modulating Intestinal Morphology and Cecum Microbiota

Effect of a probiotic containing lactobacilli and bifidobacteria on the metabolic processes, litter microbiocenosis, and production indicators of broiler Pekin ducklings

Bacillus subtilis with a 1% population enhances the activity of Enterococcus faecalis with a 99% population

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