Support for a Meiotic Recombination Initiation Complex: Interactions among Rec102p, Rec104p, and Spo11p

Jiao, Kai; Salem, Laura; Malone, Robert E.

doi:10.1128/mcb.23.16.5928-5938.2003

Cited by 44 publications

(53 citation statements)

References 46 publications

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“…5e and data not shown). These findings agree well with numerous lines of evidence indicating that these proteins work together as a functional unit (Salem et al 1999;Kee and Keeney 2002;Jiao et al 2003;Kee et al 2004). …”

Section: Requirement For the Rec102 5′ Exon For Physical Interactionssupporting

confidence: 92%

“…The findings in this study reinforce the view that has emerged from several lines of inquiry that there are at least four functionally distinct subgroups among the DSB proteins: Spo11-Ski8; Rec102-Rec104; Mer2-Mei4-Rec114; and Mre11-Rad50-Xrs2 (see Introduction) (Ohta et al 1998;Jiao et al 2003;Arora et al 2004;Kee et al 2004;Prieler et al 2005;Henderson et al 2006;Li et al 2006;Sasanuma et al 2007). To date, there is little support for the possibility that all of the DSB proteins together form a discrete "DSB holoenzyme," although available data cannot rule out this possibility.…”

Section: Functional Subgroups Among the Dsb Proteins And The Networksupporting

confidence: 87%

“…Whereas several studies document physical and functional interactions connecting Ski8, Rec102, and Rec104 to Spo11, thus far relatively few direct connections are known to link Mer2, Mei4, and Rec114 to these other factors (Salem et al 1999;Kee and Keeney 2002;Jiao et al 2003;Arora et al 2004;Kee et al 2004;Henderson et al 2006;Li et al 2006). Recent findings concerning the structure of the REC102 gene help to address this lack.…”

Section: Essential Sequence Encoded By a Previously Unrecognized 5′ Ementioning

confidence: 99%

“…In several previous studies, epitope-tagged versions of Rec102 were generated by integration of the tagging constructs at the 3′ end of the REC102 genomic locus (Kee and Keeney 2002;Jiao et al 2003;Kee et al 2004;Li et al 2006). These constructs were functional because they still had the intron and 5′ exon.…”

Section: Requirement For the Rec102 5′ Exon For Physical Interactionsmentioning

confidence: 99%

“…However, recent studies seem inconsistent with this simple model. For example, genetic, cytological, and physical analyses suggest that Rec102 and Rec104 interact to form a single functional unit (Salem et al 1999;Jiao et al 2003;Kee et al 2004). Although this Rec102-Rec104 complex interacts with other DSB proteins, including Spo11, there are numerous differences between Rec102-Rec104 and the other proteins with respect to their genetic dependencies for protein-protein and protein-chromosome interactions Wells et al 2006) (discussed in more detail below).…”

Section: Introductionmentioning

confidence: 99%

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Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae

et al. 2007

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In most sexually reproducing organisms, meiotic recombination is initiated by DNA double-strand breaks (DSBs) formed by the Spo11 protein. In budding yeast, nine other proteins are also required for DSB formation, but roles of these proteins and interactions among them are poorly understood. We report here further studies of the behaviors of these proteins. Consistent with other studies, we find that Mei4 and Rec114 bind to chromosomes from leptonema through early pachynema. Both proteins showed only limited colocalization with the meiotic cohesin subunit Rec8, suggesting that Mei4 and Rec114 associated preferentially with chromatin loops. Rec114 localization was independent of other DSB factors, but Mei4 localization was strongly dependent on Rec114 and Mer2. Systematic deletion analysis identified protein regions important for a previously described two-hybrid interaction between Mei4 and Rec114. We also report functional characterization of a previously misannotated 5′ coding exon of REC102. Sequences encoded in this exon are essential for DSB formation and for Rec102 interaction with Rec104, Spo11, Rec114, and Mei4. Finally, we also examined genetic requirements for a set of previously described two-hybrid interactions that can be detected only when the reporter strain is induced to enter meiosis. This analysis reveals new functional dependencies for interactions among the DSB proteins. Taken together, these studies support the view that Mei4, Rec114, and Mer2 make up a functional subgroup that is distinct from other subgroups of the DSB proteins: Spo11-Ski8, Rec102-Rec104, and Mre11-Rad50-Xrs2. These studies also suggest that an essential function of Rec102 and Rec104 is to connect Mei4 and Rec114 to Spo11.

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Section: Requirement For the Rec102 5′ Exon For Physical Interactionssupporting

confidence: 92%

Section: Functional Subgroups Among the Dsb Proteins And The Networksupporting

confidence: 87%

Section: Essential Sequence Encoded By a Previously Unrecognized 5′ Ementioning

confidence: 99%

Section: Requirement For the Rec102 5′ Exon For Physical Interactionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae

et al. 2007

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Spo11 and the Formation of DNA Double-Strand Breaks in Meiosis

Keeney

Genome Dynamics and Stability

311

359

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Meiotic recombination is carried out through a specialized pathway for the formation and repair of DNA double-strand breaks made by the Spo11 protein, a relative of archaeal topoisomerase VI. This review summarizes recent studies that provide insight to the mechanism of DNA cleavage by Spo11, functional interactions of Spo11 with other proteins required for break formation, mechanisms that control the timing of recombination initiation, and evolutionary conservation and divergence of these processes.

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Molecular Genetics of Recombination

Aguilera¹,

Rothstein²

2007

Topics in Current Genetics

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The biochemistry of eukaryotic homologous recombination caught fire with the discovery that Rad51 is the eukaryotic homolog of the bacterial RecA and T4 UvsX proteins; and this field is still hot. The core reaction of homologous recombination, homology search and DNA strand invasion, along with the proteins catalyzing it, are conserved throughout evolution in principle. However, the increased complexity of eukaryotic genomes and the diversity of eukaryotic cell biology pose additional challenges to the recombination machinery. It is not surprising that this increase in complexity coincided with the evolution of new recombination proteins and novel support pathways, as well as changes in the properties of those eukaryotic recombination proteins that are evidently conserved in evolution. In humans, defects in homologous recombination lead to increased cancer predisposition, underlining the importance of this pathway for genomic stability and tumor suppression. This review will focus on the mechanisms of homologous recombination in eukaryotes as elucidated by the biochemical analysis of yeast and human proteins.

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Support for a Meiotic Recombination Initiation Complex: Interactions among Rec102p, Rec104p, and Spo11p

Abstract: Initiation of meiotic recombination in the yeast

Cited by 44 publications

References 46 publications

Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae

Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae

Spo11 and the Formation of DNA Double-Strand Breaks in Meiosis

Molecular Genetics of Recombination

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