Supported ionic liquids as customizable materials to purify immunoglobulin G

Capela, Emanuel V.; Bairos, Jéssica; Pedro, Ana; Neves, Márcia C.; Aires‐Barros, M. R.; Azevedo, Ana M.; Coutinho, J.; Tavares, Ana; Freire, Mara G.

doi:10.1016/j.seppur.2022.122464

Cited by 7 publications

(4 citation statements)

References 54 publications

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Section: Figurementioning

confidence: 99%

“…In addition to nucleic acids, SILs have been applied in the purification of both monoclonal and polyclonal IgG antibodies. Capela et al (2023) investigated the use of SILs, namely with the IL ligands [C 3 C 1 im]Cl, [N 3444 ]Cl, and [N 3888 ]Cl in silica supports to capture and purify IgG from complex biological samples, i.e., human and rabbit sera and CHO cell culture supernatants. By using the 1- methyl-3-propylimidazolium-based supported material, IgG was directly recovered from the aqueous solution (diluted human serum) with a yield of 59% and a purity degree of 84%.…”

Section: Ionic-liquid-based Approaches In the Biopharmaceuticals Fieldmentioning

confidence: 99%

“…IL’s outstanding properties make them attractive for catalysis, electrochemistry, separation, and extraction, among others ( Fontanals et al, 2009 ; Kianfar and Mafi, 2020 ). Although less investigated, within the pharmaceutical field, ILs have already shown to be important enhancers of the solubility, activity, permeability, and stability of several synthetic drugs ( Pedro et al, 2020 ) and biopharmaceuticals ( Vijayaraghavan et al, 2010 ; Byrne et al, 2012 ; Foureau et al, 2012 ; Kumar and Venkatesu, 2013 ; Mukesh et al, 2013 ; Kumar and Venkatesu, 2014 ; Mazid et al, 2015 ; Todinova et al, 2016 ; Banerjee et al, 2018 ; Jagannath et al, 2018 ; Pedro et al, 2018 ; Reslan et al, 2018 ; Guncheva et al, 2019 ; Lin et al, 2019 ; Quental et al, 2019 ; Mandal et al, 2020 ; Lin et al, 2021 ; Dhiman et al, 2022 ; Vicente et al, 2022 ), and also in the extraction and purification of these biomolecules ( Shu et al, 2010 ; Qu et al, 2012 ; Yuan et al, 2012 ; Chen et al, 2015 ; Ding et al, 2015 ; Ren et al, 2015 ; Taha et al, 2015 ; Ferreira et al, 2016 ; Mondal et al, 2016 ; Ramalho et al, 2018 ; Santos et al, 2018 ; Song et al, 2018 ; Xu et al, 2018 ; Capela et al, 2019 ; Magri et al, 2019 ; Marchel et al, 2019 ; Quental et al, 2019 ; Vicente et al, 2019 ; Castro et al, 2020 ; Neves et al, 2020 ; Vicente et al, 2022 ; Capela et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

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Ionic-liquid-based approaches to improve biopharmaceuticals downstream processing and formulation

Almeida

Pedro²,

Tavares³

et al. 2023

Front. Bioeng. Biotechnol.

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The emergence of biopharmaceuticals, including proteins, nucleic acids, peptides, and vaccines, revolutionized the medical field, contributing to significant advances in the prophylaxis and treatment of chronic and life-threatening diseases. However, biopharmaceuticals manufacturing involves a set of complex upstream and downstream processes, which considerably impact their cost. In particular, despite the efforts made in the last decades to improve the existing technologies, downstream processing still accounts for more than 80% of the total biopharmaceutical production cost. On the other hand, the formulation of biological products must ensure they maintain their therapeutic performance and long-term stability, while preserving their physical and chemical structure. Ionic-liquid (IL)-based approaches arose as a promise alternative, showing the potential to be used in downstream processing to provide increased purity and recovery yield, as well as excipients for the development of stable biopharmaceutical formulations. This manuscript reviews the most important progress achieved in both fields. The work developed is critically discussed and complemented with a SWOT analysis.

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Section: Figurementioning

confidence: 99%

Section: Ionic-liquid-based Approaches In the Biopharmaceuticals Fieldmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ionic-liquid-based approaches to improve biopharmaceuticals downstream processing and formulation

Almeida

Pedro²,

Tavares³

et al. 2023

Front. Bioeng. Biotechnol.

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“…There have been many advanced researches, discoveries and patents in the field of ILs in recent years due to their significant features and great applicability, but their commercial exploitation is still limited. Ionic liquids have many unique properties, including low vapor pressure, high thermal stability, low toxicity, high ionic conductivity and almost unlimited tunability, which have already allowed them to find many applications in chemistry, electrochemistry, extraction and separation processes [ 8 , 9 , 10 , 11 , 12 , 13 , 14 ]; however, the photonic application of these materials have so far been very limited, and their optical parameters have not been sufficiently explored. Their properties can be tuned either by anion and/or cation modification, making them photodefinable, transparent, colored or flexible.…”

Section: Introductionmentioning

confidence: 99%

Optical Fiber Grating-Prism Fabrication by Imprint Patterning of Ionic-Liquid-Based Resist

Turek

Pala

Szpecht

et al. 2023

IJMS

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We present a method of microstructure fabrication on the tip of the optical fiber using a UV soft-imprint process of polymerizable ionic liquid-based optical resist. Ionic liquid with two UV-sensitive vinylbenzyl groups in the structure was diluted in non-hazardous propylene glycol (PG) to obtain liquid material for imprinting. No additional organic solvent was required. The impact of propylene glycol amount and exposure dose on optical and mechanical properties was investigated. The final procedure of the UV imprint on the optical fiber tip was developed, including the mold preparation, setup building, UV exposure and post-laser cure. As the IL-containing vinylbenzyl groups can also be polymerized by the radical rearrangement of double bonds through thermal heating, the influence of the addition of 1–2% BHT polymerization inhibitor was verified. As a result, we present the fabricated diffraction gratings and the optical fiber spectrometer component—grism (grating-prism), which allows obtaining a dispersion spectrum at the output of an optical in line with the optical fiber long axis, as the main component in an optical fiber spectrometer. The process is very simple due to the fact that its optimization already starts in the process of molecule design, which is part of the trend of sustainable technologies. The final material can be designed by the tailoring of the anion and/or cation molecule, which in turn can lead to a more efficient fabrication procedure and additional functionalities of the final structure.

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