Suppressing ROS generation by apocynin inhibited cyclic stretch-induced inflammatory reaction in HPDLCs via a caspase-1 dependent pathway

Wang, Yingying; Song, Yingshuang; Zhong, Qi; Wu, Yaqin; Zhuang, Jiabao; Qu, Fang; Xu, Chun

doi:10.1016/j.intimp.2020.107129

Cited by 11 publications

(6 citation statements)

References 55 publications

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“…We believe that the enhancement of anaerobic oxidation and the fragmentation of mitochondria are consistent with the adaptive protective response of PSCs under external stimulation to achieve stem cell maintenance. As for the alteration of ROS production under mechanical force, we found that the results of other researchers are inconsistent, which may be attributed to the divergent parameters of the force-loading [ 36 , 37 ]. Our findings may be affected by limitations such as the monotonous experimental condition of a single type of mechanical force.…”

Section: Discussionmentioning

confidence: 84%

Mechanical force induces mitophagy-mediated anaerobic oxidation in periodontal ligament stem cells

Zhang

Cui

et al. 2023

Cell Mol Biol Lett

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Background The preference for glucose oxidative mode has crucial impacts on various physiological activities, including determining stem cell fate. External mechanical factors can play a decisive role in regulating critical metabolic enzymes and pathways of stem cells. Periodontal ligament stem cells (PDLSCs) are momentous effector cells that transform mechanical force into biological signals during the reconstruction of alveolar bone. However, mechanical stimuli-induced alteration of oxidative characteristics in PDLSCs and the underlying mechanisms have not been fully elucidated. Methods Herein, we examined the expression of LDH and COX4 by qRT-PCR, western blot, immunohistochemistry and immunofluorescence. We detected metabolites of lactic acid and reactive oxygen species for functional tests. We used tetramethylrhodamine methyl ester (TMRM) staining and a transmission electron microscope to clarify the mitochondrial status. After using western blot and immunofluorescence to clarify the change of DRP1, we further examined MFF, PINK1, and PARKIN by western blot. We used cyclosporin A (CsA) to confirm the regulation of mitophagy and ceased the stretching as a rescue experiment. Results Herein, we ascertained that mechanical force could increase the level of LDH and decrease the expression of COX4 in PDLSCs. Simultaneously, the yield of reactive oxygen species (ROS) in PDLSC reduced after stretching, while lactate acid augmented significantly. Furthermore, mitochondrial function in PDLSCs was negatively affected by impaired mitochondrial membrane potential (MMP) under mechanical force, and the augment of mitochondrial fission further induced PRKN-dependent mitophagy, which was confirmed by the rescue experiments via blocking mitophagy. As a reversible physiological stimulation, the anaerobic preference of PDLSCs altered by mechanical force could restore after the cessation of force stimulation. Conclusions Altogether, our study demonstrates that PDLSCs under mechanical force preferred anaerobic oxidation induced by the affected mitochondrial dynamics, especially mitophagy. Our findings support an association between mechanical stimulation and the oxidative profile of stem cells, which may shed light on the mechanical guidance of stem cell maintenance and commitment, and lay a molecular foundation for periodontal tissue regeneration. Graphical Abstract

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Section: Discussionmentioning

confidence: 84%

Mechanical force induces mitophagy-mediated anaerobic oxidation in periodontal ligament stem cells

Zhang

Cui

et al. 2023

Cell Mol Biol Lett

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“…The DCFH-DA method was used to detect the standard of ROS in RAW264.7 cells ( 17 ). The cells were first treated with different concentrations (0, 50, 100, 200, and 400 μg/ml, 100 μl) of EPS-LP2 solution for 2 h, then stimulated with 1 μmol/L H 2 O 2 for 24 h. Following this, at room temperature, the cells were cleaned with trypsin (0.25%) until the cells were circular, and then the digestion was stopped by adding DMEM complete medium.…”

Section: Methodsmentioning

confidence: 99%

Structural, antioxidant, and immunomodulatory activities of an acidic exopolysaccharide from Lactiplantibacillus plantarum DMDL 9010

Huang

et al. 2022

Front. Nutr.

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This study investigated the structural, antioxidant, and immunomodulatory activities of acidic exopolysaccharide (EPS-LP2) isolated from Lactiplantibacillus plantarum DMDL 9010. EPS-LP2 is composed of fucose (Fuc), arabinose (Ara), galactose (Gal), glucose (Glc), mannose (Man), and D-fructose (Fru) with a molar ratio of 0.13: 0.69: 8.32: 27.57: 62.07: 0.58: 0.46, respectively. Structural analysis of EPS-LP2 exhibited a smooth irregular lamellar surface, rod-like structure with swollen ends and slippery surfaces, and good thermal stability. Based on the methylation and NMR analysis, sugar residues including t-Manp, t-Glcp, 2-Manp, 6-Galp, 6-Glcp, and 4-Glcp were found to exist in EPS-LP2. In the 50∼400 μg/ml range, EPS-LP2 showed negligible neurotoxicity to RAW264.7 cells. Moreover, EPS-LP2 could protect RAW264.7 cells from oxidative injury by lowering the generation of reactive oxygen species (ROS), malondialdehyde (MDA), and the secretion of lactate dehydrogenase (LDH). In contrast, an increase in superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and the concentrations of glutathione (GSH) were observed. Immunoreactivity assays showed that EPS-LP2 could suppress the expression of NO, tumor necrosis factor-α (TNF-α), and interleukin 6 (IL-6) and inhibit the activation of the mitogen-activated protein kinase (MAPK)/nuclear factor-κB-gene binding (NF-κB) cell pathway. Conclusively, EPS-LP2 could be a potential natural antioxidant and immunomodulatory agent in functional foods and medicines.

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“…Follow-up experiments showed that co-cultures with bone marrow mesenchymal stem cells could inhibit compressioninduced NPC apoptosis [24] . The transverse periodic stretching method uses a transverse reciprocating stretching device to provide supracellular physiological tension for constructing a disease model [25][26][27][28] . This method is also often used to build NPC degeneration models [29,30] .…”

Section: Nucleus Pulposus Cells Degeneration Model Established By a P...mentioning

confidence: 99%

Nucleus pulposus cells degeneration model: a necessary way to study intervertebral disc degeneration

Zhao

et al. 2023

Folia Morphol

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Suppressing ROS generation by apocynin inhibited cyclic stretch-induced inflammatory reaction in HPDLCs via a caspase-1 dependent pathway

Cited by 11 publications

References 55 publications

Mechanical force induces mitophagy-mediated anaerobic oxidation in periodontal ligament stem cells

Mechanical force induces mitophagy-mediated anaerobic oxidation in periodontal ligament stem cells

Structural, antioxidant, and immunomodulatory activities of an acidic exopolysaccharide from Lactiplantibacillus plantarum DMDL 9010

Nucleus pulposus cells degeneration model: a necessary way to study intervertebral disc degeneration

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