Suppression subtractive hybridization identifies bacterial genomic regions that are possibly involved in hBD‐2 regulation by enterocytes

Ghadimi, Darab; Hassan, M.; Njeru, Patrisio Njiru; Vrese, Michael de; Geis, Arnold; Shalabi, S. I.; Abdel-Razek, Sabah T.; Abdel-Khair, Abd El-Al A.; Heller, Knut J.; Schrezenmeir, Jürgen

doi:10.1002/mnfr.201100052

Cited by 11 publications

(8 citation statements)

References 28 publications

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“…), the levels of calprotectin usually are high and several times higher than the values of the healthy elderly at the end of our study [29]. Likewise, the increase of human β-defensin2 secretion was observed in healthy adult volunteers after administration of lactobacilli strains [61,62]. In these papers, the authors report that only a few strains favour the effect of the β-defensins, and they suppose that the difference among the strains is in the ability to induce the defensin expression correlated with the presence of genes encoding glycosylated cell surface structures.…”

Section: Discussionsupporting

confidence: 53%

Effects of Inulin-Based Prebiotics Alone or in Combination with Probiotics on Human Gut Microbiota and Markers of Immune System: A Randomized, Double-Blind, Placebo-Controlled Study in Healthy Subjects

et al. 2022

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The gut microbiota is implicated in diverse interactions affecting human health. The present study reports a randomized, double-blind, placebo-controlled clinical study conducted by administering a new synbiotic formulation composed of two Lactobacillus strains (L. plantarum and L. acidophilus) and one Bifidobacterium strain (B. animalis subsp. lactis) and two types of fructans (fructo-oligosaccharides with a degree of polymerization of 3–5 and inulin-type fructans with 10 DP). The effects of this synbiotic were evaluated on healthy subjects for 28 days and the maintenance of its efficacy was evaluated at the end of a follow-up period of 28 days. The synbiotic treatment contributes to higher biodiversity of the gut microbiota, increasing the community richness with respect to the group with the prebiotics alone and the placebo group. Its positive effect is also reflected in the variation of microbial community structure favoring the beneficial short-chain fatty acids bacterial producers. The amelioration of the health status of the subjects was also established by the reduction of common infectious disease symptom incidence, the stimulation of the gut immune system showing a noteworthy variation of fecal β-defensin2 and calprotectin levels, and the modulation of the response of the respiratory tract’s immune system by salivary IgA as well as total antioxidant capacity biomarkers.

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Section: Discussionsupporting

confidence: 53%

Effects of Inulin-Based Prebiotics Alone or in Combination with Probiotics on Human Gut Microbiota and Markers of Immune System: A Randomized, Double-Blind, Placebo-Controlled Study in Healthy Subjects

et al. 2022

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“…In a study where 11 lactobacilli were tested, only 2 of the strains increased hBD-2 mRNA expression in CaCo-2 cells. The difference between lactobacilli strains in their ability to induce hBD-2 correlated with the presence of genes encoding glycosylated cell surface structures [18]. Although we did not examine the probiotic used in this study for these genes, this appears a likely explanation for the absence of an effect on hBD-2 excretion in stool.…”

Section: Discussionmentioning

confidence: 99%

Effect of yoghurt containing Bifidobacterium lactis Bb12®on faecal excretion of secretory immunoglobulin A and human beta-defensin 2 in healthy adult volunteers

Kabeerdoss

Devi

Mary

et al. 2011

Nutr J

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BackgroundProbiotics are used to provide health benefits. The present study tested the effect of a probiotic yoghurt on faecal output of beta-defensin and immunoglobulin A in a group of young healthy women eating a defined diet.Findings26 women aged 18-21 (median 19) years residing in a hostel were given 200 ml normal yoghurt every day for a week, followed by probiotic yoghurt containing Bifidobacterium lactis Bb12® (109 in 200 ml) for three weeks, followed again by normal yoghurt for four weeks. Stool samples were collected at 0, 4 and 8 weeks and assayed for immunoglobulin A and human beta-defensin-2 by ELISA. All participants tolerated both normal and probiotic yoghurt well. Human beta-defensin-2 levels in faeces were not altered during the course of the study. On the other hand, compared to the basal sample, faecal IgA increased during probiotic feeding (P = 0.0184) and returned to normal after cessation of probiotic yoghurt intake.ConclusionsBifidobacterium lactis Bb12® increased secretory IgA output in faeces. This property may explain the ability of probiotics to prevent gastrointestinal and lower respiratory tract infections.

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“…Specifically some heat-killed strains exhibited β-defensin induction comparable to viable bacteria (Rizzo et al, 2013;Wehkamp et al, 2004). Induction of β-defensin expression in epithelial cells by bacterial components from probiotics or commensal microorganisms has also been previously reported (Bhattacharyya et al, 2016;Ghadimi et al, 2011;Gupta et al, 2010;Krisanaprakornkit, Kimball, & Dale, 2002;Li et al, 2013;Schlee et al, 2007;Schlee et al, 2008;Schmitt et al, 2015;Zhang, Jin, & Yang, 2019). Probiotics are generally viable beneficial microbes that confer a health benefit on the host.…”

Section: Discussionmentioning

confidence: 77%

Toll-like receptor 2-mediated induction of avian β -defensin 9 by Lactobacillus rhamnosus and its cellular components in chicken intestinal epithelial cells

Jia

Wei

Hong

et al. 2019

Food and Agricultural Immunology

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This study aimed to investigate the effects of probiotic Lactobacillus rhamnosus MLGA and its cell wall components on the induction of avian β-defensin 9 (AvBD9) expression in intestinal epithelial cells (IECs) from chicken embryos and to elucidate the underlying mechanism. Chicken IECs were stimulated with L. rhamnosus MLGA or cell wall components. Quantitative RT-PCR analysis was used to determine the expressions of target genes. L. rhamnosus MLGA and its cellular components significantly induced AvBD9 and TLR2 expression, with the whole peptidoglycan (WPG) being the most potent inducer. Pretreatment of chicken IECs with a TLR2 neutralizing antibody diminished AvBD9 induction by L. rhamnosus MLGA and WPG. The inhibition tests showed that induction of AvBD9 in IECs by L. rhamnosus MLGA and WPG was dependent on NF-κB or JNK pathways. These results demonstrate that L. rhamnosus MLGA and WPG induce AvBD9 expression in chicken IECs via a TLR2-mediated NF-κB/JNK pathway.

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Suppression subtractive hybridization identifies bacterial genomic regions that are possibly involved in hBD‐2 regulation by enterocytes

Cited by 11 publications

References 28 publications

Effects of Inulin-Based Prebiotics Alone or in Combination with Probiotics on Human Gut Microbiota and Markers of Immune System: A Randomized, Double-Blind, Placebo-Controlled Study in Healthy Subjects

Effects of Inulin-Based Prebiotics Alone or in Combination with Probiotics on Human Gut Microbiota and Markers of Immune System: A Randomized, Double-Blind, Placebo-Controlled Study in Healthy Subjects

Effect of yoghurt containing Bifidobacterium lactis Bb12®on faecal excretion of secretory immunoglobulin A and human beta-defensin 2 in healthy adult volunteers

Toll-like receptor 2-mediated induction of avian β -defensin 9 by Lactobacillus rhamnosus and its cellular components in chicken intestinal epithelial cells

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