Suppressive Effect of Fruiting Bodies of Medicinal Mushrooms on Demyelination and Motor Dysfunction in a Cuprizone-Induced Multiple Sclerosis Mouse Model

Yamashina, Kota; Yamamoto, Shinji; Matsumoto, Mitsuomi; Iwasa, Kenji; Takeda, Nonoka; Haruta, Chikara; Maruyama, Kei; Shimizu, Kuniyoshi; Yoshikawa, Ken

doi:10.1615/intjmedmushrooms.2022044840

Cited by 4 publications

(2 citation statements)

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“…All reagents used were of the highest grade and were purchased from Sigma-Aldrich (St. Louis, MO, USA), Carlo Erba (Milan, Italy), Bio-Rad Laboratories (Segrate, Italy) and GE Healthcare (Uppsala, Sweden). The DI-TNC1 (ATCC CRL-2005) cell line, established from primary cultures of type 1 astrocytes from brain diencephalon tissue of 1-day-old rats [39], was acquired and authenticated from the ATCC (www.lgcstandards-atcc.org). Dulbecco's modified Eagle's medium (DMEM), trypsin, penicillin and streptomycin were provided by GIBCO (Paisley, Scotland).…”

Section: Chemicalsmentioning

confidence: 99%

“…Thanks to this property, polysaccharides could mitigate the toxicity associated with ROS overexpression and provide a potential therapeutic approach in the prevention of various diseases [35,36]. Furthermore, the interest in the biological activity of polysaccharides has increased since both in vitro and in vivo studies demonstrated that they possess neuroprotective activities, justifying their use for the prevention of neurological diseases and for the improvement of the effectiveness of conventional treatments [37][38][39]. Here, an enriched polysaccharide fraction (EPF) from the powdered fruiting body of P. eryngii was extracted and used in different assays to characterize its antioxidant and neuroprotective properties.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant Activity of Polysaccharides from the Edible Mushroom Pleurotus eryngii

et al. 2023

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In this study the antioxidant and neuroprotective activity of an enriched polysaccharide fraction (EPF) obtained from the fruiting body of cultivated P. eryngii was evaluated. Proximate composition (moisture, proteins, fat, carbohydrates and ash) was determined using the AOAC procedures. The EPF was extracted by using, in sequence, hot water and alkaline extractions followed by deproteinization and precipitation with cold ethanol. Total α- and β-glucans were quantified using the Megazyme International Kit. The results showed that this procedure allows a high yield of polysaccharides with a higher content of (1-3; 1-6)-β-D-glucans. The antioxidant activity of EPF was detected from the total reducing power, DPPH, superoxide, hydroxyl and nitric oxide radical scavenging activities. The EPF was found to scavenge DPPH, superoxide, hydroxyl and nitric oxide radicals with a IC50 values of 0.52 ± 0.02, 1.15 ± 0.09, 0.89 ± 0.04 and 2.83 ± 0.16 mg/mL, respectively. As assessed by the MTT assay, the EPF was biocompatible for DI-TNC1 cells in the range of 0.006–1 mg/mL and, at concentrations ranging from 0.05 to 0.2 mg/mL, significantly counteracted H2O2-induced reactive oxygen species production. This study demonstrated that polysaccharides extracted from P. eryngii might be used as functional food to potentiate the antioxidant defenses and to reduce oxidative stress.

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Section: Chemicalsmentioning

confidence: 99%