Supramolecular interaction of transition metal complexes with albumins and DNA: Spectroscopic methods of estimation of binding parameters

Abstract

Binding parameters of metal complexes with albumins and DNA since the middle of 1990s are considered and summarized. The most widespread spectroscopic methods of estimation of binding parameters are discusseddirect fluorescent methods, indirect fluorescent methods (by fluorescence quenching parameters), and ways of estimation of binding constants by other optical spectroscopic methods. The methods and approaches to calculations used for the determination of binding constants are discussed. The data on the already found binding constants and kinetic parameters is systematized.KEYWORDS albumins, DNA, metal complexes, proteins, supramolecular interaction Funding information: Russian Foundation for Basic Research, Grant/Award Number: 16-03-01089_a 16-53-50027_YaF_a Abbreviations and designations used: F 0 , fluorescence intensity in the absence of a quencher; F, fluorescence intensity in the presence of a acid); NTSC, a new thiosemicarbazone; o-cbiaH, 5-(2-carboxybenzyloxy)isophthalic acid; o-van-gln, a Schiff base derived from o-vanillin and glutamine; o-van-L-met, Schiff base derived from o-vanillin and L-methionine; oxo, 5-

Misinterpretations in Evaluating Interactions of Vanadium Complexes with Proteins and Other Biological Targets

“…Another problem with this methodology is that the binding may involve the formation of both a 1:1 and 1:2 (V IV O:L) complexes, the treatment described below not being valid in these cases. Conventionally, when molecules bind independently to a set of equivalent sites on a macromolecule, the equilibrium between free and bound molecules may be given by the following equation [41,42],…”

“…A solution of apoHTF with concentration 1.02 × 10 −6 M was prepared, and a solution of [V IV O(acac) 2 ] was progressively added to get solutions with C V from 0 to 1.8 × 10 −5 M, i.e., with [V IV O(acac) 2 ]:HTF ratios from 1 to~18. Following the usual linearization methodologies (as described above) [41,42,44], it was assumed that the fluorescence quenching observed should be due to binding of the [V IV O(acac) 2 ] complex to apoHTF, thus a static quenching is operating which is due to the non-emitting [V IV O(acac) 2 ] bound to the protein. Using Equation ( 8), the values of K 2 BC = 1.0 × 10 4 and n = 1.15 were obtained.…”

“…Besides the ability to cleave/damage DNA, the binding interactions of vanadium complexes to DNA have also been studied by different techniques; for example electronic absorption [56,[59][60][61][62], circular dichroism [60][61][62], and fluorescence emission have been used frequently [42,[60][61][62]65]; other techniques such as atomic force microscopy [66][67][68][69], viscosity [56,[59][60][61]69] and have also been applied. 51 V NMR, [53,54] capillary electrophoresis and Fourier transform infrared difference spectroscopy [70] has also been used, but much less frequently, as well as DNA melting, applied to evaluate e.g., binding and/or DNA crosslink formation by the complexes [56,59,60,71].…”

Vanadium(v) complexes derived from triphenylphosphonium and hydrazides: cytotoxicity evaluation and interaction with biomolecules

“…25,26 The affinity with HSA might be considered a decisive step in approving and applying a new medicine. 27 There are also reports on the synthesis and structural characterization of vanadium(V) complexes containing tridentate hydrazone ligands with O,N,O topology, as well as their corresponding interaction with both DNA and serum albumin. [28][29][30][31] Notably, our group has synthesized and evaluated the interactive profile with DNA and HSA of some vanadium-based compounds, e.g., two series of vanadium(V) complexes derived from pyridoxal hydrochloride or salicylaldehyde and p-substituted benzoic hydrazides.…”

Synthesis, Spectral Characterization, Computational Revisions, Toxicology Studies In Addition To Biotic Studies of O-Phenylenediamine Based Schiff Base and Its Transition Metal Complexes

“…It was well known that some co-ordination compounds may inhibit cancer cell multiplication due to binding and damage to cancer DNA 2,4,14 . It was requisite en route for pattern metallic multiplexes by way of a reduced amount of lethal, target-peculiar as well as spending non-covalent binding modus in the direction of DNA 15 .…”