Surface-active potential of biosurfactants produced in curd whey by Pseudomonas aeruginosa strain-PP2 and Kocuria turfanesis strain-J at extreme environmental conditions

Dubey, Kirti; Charde, Pravin; Meshram, Sudhir U.; Shendre, Latika P.; Dubey, Vijay S.; Juwarkar, Asha A.

doi:10.1016/j.biortech.2012.05.024

Cited by 38 publications

(17 citation statements)

References 27 publications

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“…The surface activity remained higher even at the highest salt concentration of (30 % w/v), by which it was not effected, and it exhibited high stability. Some reports have confirmed similarly good stabilities of biosurfactants produced by Bacillus and Pseudomonas aeruginosa strains [18,29,30]. These properties of biosurfactants make them an exceptional candidate for application in microbial enhanced oil recovery, since biosurfactants are excellent alternatives to chemical surfactants that are toxic, non-degradable and less activated at 4%-5% salinity, as discussed by Bognolo et al [31].…”

Section: Effect Of Environmental Conditions On Growth and Stabilitymentioning

confidence: 79%

Production and Application of Biosurfactant Produced by Bacillus licheniformis Ali5 in Enhanced Oil Recovery and Motor Oil Removal from Contaminated Sand

Ali

Wang

et al. 2019

Molecules

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The present study describes the production of biosurfactant from isolate B. licheniformis Ali5. Seven different, previously-reported minimal media were screened for biosurfactant production, and two selected media were further optimized for carbon source. Further, various fermentation conditions such as (pH 2–12, temperature 20–50 °C, agitation speed 100–300 rpm, NaCl (0–30 g·L−1) were investigated. The partially purified biosurfactant was characterized by Fourier transform infrared spectroscopy (FTIR) and matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF MS) and found a lipopeptide mixture, similar to lichenysin-A. Biosurfactant reduced surface tension from 72.0 to 26.21 ± 0.3 and interfacial tension by 0.26 ± 0.1 mN·m−1 respectively, biosurfactant yield under optimized conditions was 1 g·L−1, with critical micelle concentration (CMC) of 21 mg·L−1 with high emulsification activity of (E24) 66.4 ± 1.4% against crude oil. Biosurfactant was found to be stable over extreme conditions. It also altered the wettability of hydrophobic surface by changing the contact angle from 49.76° to 16.97°. Biosurfactant efficiently removed (70-79%) motor oil from sand, with an efficiency of more than 2 fold as compared without biosurfactant (36–38%). It gave 32% additional oil recovery over residual oil saturation upon application to a sand-packed column. These results are indicative of potential application of biosurfactant in wettability alteration and ex-situ microbial enhanced oil recovery.

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Section: Effect Of Environmental Conditions On Growth and Stabilitymentioning

confidence: 79%

Production and Application of Biosurfactant Produced by Bacillus licheniformis Ali5 in Enhanced Oil Recovery and Motor Oil Removal from Contaminated Sand

Ali

Wang

et al. 2019

Molecules

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“…In recent decades, several novel biosurfactant producers have been discovered and was used as no-cost complete nutrient medium viz. distillery wastewater, curd whey waste, fruit processing waste for biosurfactant production (Juwarkar et al 2008;Dubey et al 2012a). Some microorganisms, mainly from genera Pseudomonas and Mycobacterium, were capable of oxidizing petroleum products and trace of organic acids (Zobell 1946).…”

Section: Responsible Editor: Robert Duranmentioning

confidence: 99%

Biosurfactant production by Pseudomonas aeruginosa DSVP20 isolated from petroleum hydrocarbon-contaminated soil and its physicochemical characterization

Sharma

Ansari

Al-Ghamdi

et al. 2015

Environ Sci Pollut Res

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Among 348 microbial strains isolated from petroleum hydrocarbon-contaminated soil, five were selected for their ability to produce biosurfactant based on battery of screening assay including hemolytic activity, surface tension reduction, drop collapse assay, emulsification activity, and cell surface hydrophobicity studies. Of these, bacterial isolate DSVP20 was identified as Pseudomonas aeruginosa (NCBI GenBank accession no. GQ865644) based on biochemical characterization and the 16S rDNA analysis, and it was found to be a potential candidate for biosurfactant production. Maximum biosurfactant production recorded by P. aeruginosa DSVP20 was 6.7 g/l after 72 h at 150 rpm and at a temperature of 30 °C. Chromatographic analysis and high-performance liquid chromatography-mass spectrometry (HPLC-MS) revealed that it was a glycolipid in nature which was further confirmed by nuclear magnetic resonance (NMR) spectroscopy. Bioremediation studies using purified biosurfactant showed that P. aeruginosa DSVP20 has the ability to degrade eicosane (97%), pristane (75%), and fluoranthene (47%) when studied at different time intervals for a total of 7 days. The results of this study showed that the P. aeruginosa DSVP20 and/or biosurfactant produced by this isolate have the potential role in bioremediation of petroleum hydrocarbon-contaminated soil.

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“…In addition, the greater chemical diversity of biosurfactants seems to be the key to the more versatile application of these molecules than chemical surfactants to provide a greater number of biotechnological applications . These advantages include higher interfacial and surface tension‐reducing activities; high stability (thermal tolerance and resistance to a wider pH range and ionic strength oscillations), which permit their use under extreme conditions; biodegradability; and reduced cytotoxicity …”

Section: Introductionmentioning

confidence: 99%

Properties of novel surfactin‐derived biosurfactants obtained through solid‐phase synthesis

Vázquez

Ferreira

Cavalcante

et al. 2018

Journal of Peptide Science

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Eight molecules, four peptides (SPs) and four lipopeptides (LPs) derived by rational design from surfactin, a well‐known secreted biosurfactant from Bacillus subtilis, were produced employing Fmoc‐based solid‐phase synthesis. These new peptides were tested to evaluate their potential biosurfactant and biological activities, aiming at possible applications in industrial, biological, pharmaceutical, and medical use. Five molecules (SP1, SP2, SP4, LP5, and LP8) presented potential for medical uses, mainly due to their drug delivery properties as suggested by their synergistic activity with the antibiotic vancomycin against Staphylococcus aureus. All synthetic peptides showed low toxicity against Vero cell cultures, in assays of hemolysis, and in different cytotoxicity assays. In addition, we found that three peptides (SP1, LP6, and LP7) had potential technological and industrial use because of their emulsifying capacity, low toxicity, and ability to physically stabilize solutions. These novel molecules retained some properties of the parental molecule (surfactin, which was originally obtained through nonribosomal synthesis in Bacillus subtilis) but have the advantage of being linear peptides, which can be produced at large scales through the use of conventional heterologous protein expression protocols.

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Surface-active potential of biosurfactants produced in curd whey by Pseudomonas aeruginosa strain-PP2 and Kocuria turfanesis strain-J at extreme environmental conditions

Cited by 38 publications

References 27 publications

Production and Application of Biosurfactant Produced by Bacillus licheniformis Ali5 in Enhanced Oil Recovery and Motor Oil Removal from Contaminated Sand

Production and Application of Biosurfactant Produced by Bacillus licheniformis Ali5 in Enhanced Oil Recovery and Motor Oil Removal from Contaminated Sand

Biosurfactant production by Pseudomonas aeruginosa DSVP20 isolated from petroleum hydrocarbon-contaminated soil and its physicochemical characterization

Properties of novel surfactin‐derived biosurfactants obtained through solid‐phase synthesis

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