Surface adhered poly(vinyl alcohol) physical hydrogels as tools for rational design of intelligent biointerfaces

Jensen, Bettina E. B.; Alves, Marie-Hélène; Fejerskov, Betina; Städler, Brigitte; Zelikin, Alexander N.

doi:10.1039/c2sm07075h

Cited by 25 publications

(58 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The device can also be removed easily from the heart, when the PVA dissolved in the body. The adhesion strength, duration of contact and conformability of the device can be controlled by varying the molecular weight and degree of saponification of PVA 12,13 . Furthermore, the gel electrodes can establish good adhesion on finger joints without necessitating the use of other adhesive materials.…”

mentioning

confidence: 99%

A strain-absorbing design for tissue–machine interfaces using a tunable adhesive gel

Lee¹,

Inoue²,

Kim³

et al. 2014

Nat Commun

127

View full text Add to dashboard Cite

mentioning

confidence: 99%

A strain-absorbing design for tissue–machine interfaces using a tunable adhesive gel

Lee¹,

Inoue²,

Kim³

et al. 2014

Nat Commun

127

View full text Add to dashboard Cite

“…lack of physical hydrogelation. Previously, we showed that PVA hydrogelation can be induced via a number of non--cryogenic techniques, 27 specifically through incubation in mixtures of water with isopropanol. 27 …”

Section: Resultsmentioning

confidence: 99%

“…In contrast, hydrogels prepared using blends of PVA with collagen (as well as chitosan, poly--L--lysine, etc) support cell adhesion and proliferation. 27 We aimed to apply this facile materials design strategy to hydrogel nanoparticles and in assembly of HNP used solutions of PVA containing collagen at a 0.1 g/L concentration. For each PVA molecular weight, collagen--containing HNP exhibited a size similar to pristine, PVA--only counterparts, polydispersity indeces at or below 0.3, and near zero zeta potential ( Figure 3).…”

Section: +Particlementioning

confidence: 99%

“…Polymer molecular weight is a characteristic decisive in diverse properties of biomaterials and specifically for PVA, we have shown that physical hydrogels derived thereof exhibit markedly differed elasticity when prepared using polymer samples with varied molecular weights. 26,27 Furthermore, in our recent report we have used molecular weight of PVA as a tool of effective control over spontaneous degradation of physical hydrogels in physiological conditions. 28 Finally, retention and elimination of PVA from the human body is to large extent controlled by the polymer molecular weight.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Poly(vinyl alcohol) Physical Hydrogel Nanoparticles, Not Polymer Solutions, Exert Inhibition of Nitric Oxide Synthesis in Cultured Macrophages

et al. 2013

Self Cite

View full text Add to dashboard Cite

Hydrogel nanoparticles (HNP) are an emerging tool of biomedicine with unique materials characteristics, scope, and utility. These hydrated, soft colloidal carriers can penetrate through voids with dimensions narrower than the size of the particle, provide stabilization for fragile biological cargo and allow diffusion and exchange of solutes with external phase. However, techniques to assemble HNP are few; solitary examples exist of biocompatible polymers being formulated into HNP; and knowledge on the biomedical properties of HNP remains rather cursory. In this work, we investigate assembly of HNP based on a polymer with decades of prominence in the biomedical field, poly(vinyl alcohol), PVA. We develop a novel method for production of PVA HNP through nanoprecipitation-based assembly of polymer nanoparticles and subsequent physical hydrogelation of the polymer. Polymer nanoparticles and HNP were visualized using scanning electron microscopy and fluorescence imaging, and characterized using dynamic light scattering and zeta potential measurements. Interaction of PVA HNP with mammalian cells was investigated using flow cytometry, viability screening, and measurements of nitric oxide production by cultured macrophages. The latter analyses revealed that PVA administered as a polymer solution or in the form of HNP resulted in no measurable increase in production of the inflammation marker. Unexpectedly, PVA HNP exerted a pronounced inhibition of NO synthesis by stimulated macrophages, that is, had an anti-inflammatory activity. This effect was accomplished with a negligible change in the cell viability and was not observed when PVA was administered as a polymer solution. To the best of our knowledge, this is the first observation of inhibition of NO synthesis in macrophages by administered nanoparticles and specifically hydrogel nanoparticles. Taken together, our results present PVA HNP as promising colloidal hydrogel nanocarriers for biomedical applications, specifically drug delivery and assembly of intracellular biosensors. Corresponding author : E-mail: zelikin@chem.au.dk ABSTRACT Hydrogel nanoparticles (HNP) are an emerging tool of biomedicine with unique materials characteristics, scope, and utility. These hydrated, soft colloidal carriers can penetrate through voids with dimensions narrower than the size of the particle, provide stabilization for fragile biological cargo, and allow diffusion and exchange of solutes with external phase. However, techniques to assemble HNP are few; solitary examples exist of biocompatible polymers being formulated into HNP; and knowledge on the biomedical properties of HNP remains rather cursory. In this work, we investigate assembly of HNP based on a polymer with decades of prominence in the biomedical filed, poly(vinyl alcohol), PVA. We develop a novel method for production of PVA HNP through nanoprecipitation -based assembly of polymer nanoparticles and subsequent physical hydrogelation of the polymer. Polymer nanoparticles and HNP were visualized using scanning ele...

show abstract

“…Pristine PVA has low protein adsorption [35] and inhibits cell attachment [36], and cell adhesive areas can be made by patterning PVA films. Micropatterning methods of PVA include micro photoablation [37], micromolding on surfaces [38] or in capillaries [39], UV light-enabled degradation [36], photo-crosslinking [40,41] or inkjet printing of oxidizing solutions [36]. However, so far the available patterning methods are still somewhat limited in either resolution, chemical functionality or by the complex chemistry and instrumentation involved.…”

Section: Introductionmentioning

confidence: 99%

Patterned cell arrays and patterned co-cultures on polydopamine-modified poly(vinyl alcohol) hydrogels

Beckwith

Sikorski

2013

Biofabrication

View full text Add to dashboard Cite

Abstract. Live cell arrays are an emerging tool that expand traditional 2D in vitro cell culture, increasing experimental precision and throughput. A patterned cell system was developed by combining the cell repellent properties of polyvinyl alcohol hydrogels with the cell adhesive properties of self-assembled films of dopamine (polydopamine). It was shown that polydopamine could be patterned onto polyvinyl alcohol hydrogels by microcontact printing, which in turn effectively patterned the growth of several cell types (HeLa, HEK293, HUVEC and PC3). The cells could be patterned at levels down to single-cell confinement, and it was demonstrated that cell patterns could be maintained for at least 3 weeks. Further, polydopamine could be used to modify poly(vinyl alcohol) in situ using a cell compatible deposition buffer (1 mg mL −1 dopamine in 25 mm tris with a physiological salt balance). The treatment switched the PVA hydrogel from cell repellent to cell adhesive. By pre-patterning one cell population and depositing polydopamine in situ, a second cell population could be seeded, and adhered to the newly switched surface. Patterned co-cultures of HeLa/HeLa and HeLa/HUVEC were thus realized through simple chemistry and could be studied over time. The combination of polyvinyl alcohol and polydopamine was shown to be an attractive route to versatile live cell arrays with minimal infrastructure requirements and low experimental complexity.PACS numbers: 87.17. Rt, 87.18.Gh, 87.80.Fe Keywords: Cell micropatterning, cell arrays, polydopamine, poly(vinyl alcohol) hydrogels, micropatterned co-cultures Patterned cell arrays on polydopamine-modified poly(vinyl alcohol) hydrogels.

show abstract

Surface adhered poly(vinyl alcohol) physical hydrogels as tools for rational design of intelligent biointerfaces

Cited by 25 publications

References 38 publications

A strain-absorbing design for tissue–machine interfaces using a tunable adhesive gel

A strain-absorbing design for tissue–machine interfaces using a tunable adhesive gel

Poly(vinyl alcohol) Physical Hydrogel Nanoparticles, Not Polymer Solutions, Exert Inhibition of Nitric Oxide Synthesis in Cultured Macrophages

Patterned cell arrays and patterned co-cultures on polydopamine-modified poly(vinyl alcohol) hydrogels

Contact Info

Product

Resources

About