Surface and stability analysis of immobilized laccase on poly(ethylene terephthalate) grafted maleic anhydride nanofiber mat

Abstract

BACKGROUND: A poly(ethylene terephthalate) (PET) based carrier was used for enzyme immobilization due to its good physical and mechanical properties. However, its high hydrophobicity and chemically inactive properties hindered its capability as an enzyme carrier. This study developed a PET-based carrier by grafting PET with maleic anhydride (MAH) before electrospinning. This carrier was used to immobilize laccase and characterized based on its stability after immobilization.RESULTS: The highest laccase activity recovery for laccase immobilized on PET-g-MAH nanofiber mat was 59.17% using covalent bonding, followed by the crosslinking method. The static water contact angle of PET-g-MAH nanofiber mats was reduced from 129 ± 8 o to 109 ± 12 o . Based on the Wenzel model, the reduction was due to the decrease in surface roughness of the hydrophobic surface. Besides, the immobilized laccase also withstood high temperature up to 60 °C and retained about 29.22% ± 5.06% of its initial activity after 30 days of storage and repeated use.CONCLUSION: Laccase immobilization on PET-g-MAH nanofiber has good stability (thermal, storage and reusability) and is recommended to be implemented in the industries.

An Alternative Approach to Plastic Recycling: Fabrication and Characterization of rPET/CA Nanofiber Carriers to Enhance Porcine Pancreatic Lipase Stability Properties

“…PPL was first immobilized on rPET/CA nanofibers using adsorption and then cross-linking methods with GA. In the literature, it has been reported that trypsin, laccase, and lipase were immobilized on PET-based nanofibers. ,,, However, none of these studies employed waste materials as the source of PET for their experiments. This study differs from its counterparts in the literature in that wastewater bottles are used as PET sources in the production of PET-based carrier nanofibers in PPL immobilization.…”

Efficient Electroenzymatic Cascade Catalysis for Ortho-Selective Chlorination of Phenol

“…So, the two processes are difficult to be compatible in one pot . Moreover, the immobilization of enzyme on a solid carrier often results in a decrease of catalytic activity compared to the one of the free enzyme due to the increase of diffusion resistance of substrate on a solid carrier than that in bulk solution. , How to enhance the activity of immobilized enzymes, as well as the electron transfer efficiency between the enzyme and the electrode should also be taken into account.…”