Surface Characteristics of the Human K (Killer) Lymphocyte

Eremin, O.; Kraft, D; Coombs, R.R.A.; Franks, D.; Ashby, J.; Plumb, D.

doi:10.1159/000231917

Cited by 17 publications

(8 citation statements)

References 11 publications

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“…The percentage of (Fc)IgG-receptor C3-receptor-and Ig-bearing subsets were comparable to the values determined for the small cells as a whole or within each tumour preparation. As with the small cells, some rosettes (25 + 27%) were formed with control indicator cells in the DARR assay (Coombs et al, 1977) suggesting a macrophage-like reactivity. Many of the rosetting cells (20-60%) resembled macrophages morphologically, but it was not possible, because of the (Table II) prepared either by mechanical disaggregation or by short-term collagenase digestion.…”

Section: Lymphoreticular Cells In Human Brain Tumoursmentioning

confidence: 74%

“…In contrast to our findings, were not able to detect C3-receptor-bearing macrophages. These authors, on the other hand, used trypsin to prepare their tumour cell suspensions-a procedure known to readily remove C3 receptor from the cell surface (Eremin et al, 1977). Macroglial cells (Glees et al, 1978) and tumour cells (Kerr & Searle, 1972) have been documented expressing phagocytic activity, and therefore could possibly possess surface receptors for Fc and C3.…”

Section: Discussionmentioning

confidence: 99%

“…Cell-8urface markers.-The various lymphocyte subsets and surface-marker characteristics of macrophages were determined using techniques whose methodology has been described (Eremin et al, 1976;Coombs et al, 1977). Briefly, the thymus-derived T lymphocytes were detected by the non-specific sheep red blood cell (SRBC) rosettng and the surface immunoglobulin (sIg-) bearing B lymphocytes by the direct antiglobulin rosetting reaction (DARR).…”

Section: Methodsmentioning

confidence: 99%

“…The nonlymphocyte small rosetting cells appeared to be non-malignant, as assessed by bright-field illumination and electron microscopy, and were regarded as being microglial (see below). In the brain tumour-cell preparations a variable percentage (15 + 14%) of the small cells also rosetted with control indicator cells (normal rabbit immunoglobulin chromic chloride linked to trypsin-treated ox RBC) and therefore behaved like macrophages (Coombs et al, 1977).…”

Section: Lymphoreticular Cells In Human Brain Tumoursmentioning

confidence: 99%

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Lymphoreticular cells in human brain tumours and in normal brain

Phillips¹,

Eremin²,

Anderson³

1982

Br J Cancer

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Summary.-The present investigation, using various rosetting assays of cell suspensions prepared by mechanical disaggregation or collagenase digestion, demonstrated lymphoreticular cells in human normal brain (cerebral cortex and cerebellum) and in malignant brain tumours. The study revealed T and B lymphocytes and their subsets (bearing receptors for Fc(IgG) and C3) in 5/14 glioma suspensions, comprising < 15% of the cell population. Between 20-60% of cells in tumour suspensions morphologically resembled macrophages and s75%0 of these cells formed strong rosettes.Lymphocytes were not found in cancer-free (putatively normal) brain. Macrophages and the smaller "microglial cells" (both phagocytic, staining with sudan black, and expressing Fc(IgG) and C3 receptors) were found in normal brain in numbers similar to those in tumour suspensions, but with less rosetting avidity. These cells may be part of an immunological defence mechanism.

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Section: Lymphoreticular Cells In Human Brain Tumoursmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Lymphoreticular Cells In Human Brain Tumoursmentioning

confidence: 99%

See 2 more Smart Citations

Lymphoreticular cells in human brain tumours and in normal brain

Phillips¹,

Eremin²,

Anderson³

1982

Br J Cancer

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“…A clear definition of the nature of lymphocyte sub sets responsible for ADCC in different spe cies has been the goal of many investigators (4, 7, 12-14, 21, 28, 31, 39, 40], Some of the earlier studies had claimed that lym phoid K cells possess B cell characteristics [9,42,44] but the bulk of evidence favors the hypothesis that a null lymphocyte (e.g., neither B nor T) is the predominant K cell in most species. However, some recent stud ies have suggested that T cells may act as K cells against some targets [5,11,20,28,37,38], and the human B cell was clearly shown to act as a K cell against lymphoblastoid cell targets by Eremin et al [7].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Bovine Leukocytes Involved in Antibody-Dependent Cell Cytotoxicity

Grewal¹,

Rouse²

1979

Int Arch Allergy Immunol

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By the use of cell surface markers combined with physical separation techniques, bovine mononuclear leukocytes active as K cells in antibody-dependent cell cytotoxicity were characterized. The most active cells were glass wool adherent and were presumed to be macrophages. In the glass wool nonadherent population, heterogeneity was detected with respect to the complement (C) receptor but all cells expressed the Fc receptor. The C receptor-positive population adhered to nylon wool but was not a B cell and was assumed to be monocyte. The C receptor-negative population, the true lymphoid K cell, was nonadherent to nylon and was suggested to be an Fc receptor-positive null lymphocyte. The results were compared to those obtained with other species.

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Human natural cytotoxicity in the blood and lymphoid organs of healthy donors and patients with malignant disease

Eremin

Ashby

Stephens

1978

Intl Journal of Cancer

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Natural cytotoxicity against CLA-4 and D6 target cells was determined in the peripheral blood of healthy donors and women with mammary carcinoma (localized to the breast and axilla), and in human normal and regional tumour lymph nodes. The NK cell activity in the blood of women with clinically localized mammary carcinoma showed a wide range of reactivities but was similar to the pattern detected in healthy controls. The NK cell activity in human normal lymph nodes also showed a wide range and was the same irrespective of the anatomical site of origin of the lymph node. Lymph nodes draining a variety of solid tumours had a similar spectrum of NK cell activity, there being no obvious correlation between the level of NK cell activity and such parameters as size and histological type of tumour, hyperplasia or metastatic spread in the regional node. Trypsin treatment of lymphocyte suspensions from the various lymphoid compartments suggested that the cell responsible for natural cytotoxicity in blood was probably different from the NK cell in tonsil and lymph nodes.

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Surface Characteristics of the Human K (Killer) Lymphocyte

Cited by 17 publications

References 11 publications

Lymphoreticular cells in human brain tumours and in normal brain

Lymphoreticular cells in human brain tumours and in normal brain

Characterization of Bovine Leukocytes Involved in Antibody-Dependent Cell Cytotoxicity

Human natural cytotoxicity in the blood and lymphoid organs of healthy donors and patients with malignant disease

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