Surface Chemistry of Bacteriophage and Laser Ablated Nanoparticle Complexes for Pathogen Detection

Tawil, Nancy; Sacher, E.; Rooney, David; Mandeville, Rosemonde; Meunier, Michel

doi:10.1021/acs.jpcc.5b02169

Cited by 9 publications

(4 citation statements)

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“…When it comes to analysis of viruses and phages using XPS, very few studies exist and they mainly deal with applications in bioengineering using phages as a tool (Tawil et al, 2013;Tawil et al, 2015;Rho et al, 2018). To our knowledge, XPS has not previously been used in studies focusing on understanding the surface chemistry of viruses.…”

Section: Virus Surface Chemistrymentioning

confidence: 99%

Applying Cryo-X-ray Photoelectron Spectroscopy to Study the Surface Chemical Composition of Fungi and Viruses

et al. 2021

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Interaction between microorganisms and their surroundings are generally mediated via the cell wall or cell envelope. An understanding of the overall chemical composition of these surface layers may give clues on how these interactions occur and suggest mechanisms to manipulate them. This knowledge is key, for instance, in research aiming to reduce colonization of medical devices and device-related infections from different types of microorganisms. In this context, X-ray photoelectron spectroscopy (XPS) is a powerful technique as its analysis depth below 10 nm enables studies of the outermost surface structures of microorganism. Of specific interest for the study of biological systems is cryogenic XPS (cryo-XPS). This technique allows studies of intact fast-frozen hydrated samples without the need for pre-treatment procedures that may cause the cell structure to collapse or change due to the loss of water. Previously, cryo-XPS has been applied to study bacterial and algal surfaces with respect to their composition of lipids, polysaccharides and peptide (protein and/or peptidoglycan). This contribution focuses onto two other groups of microorganisms with widely different architecture and modes of life, namely fungi and viruses. It evaluates to what extent existing models for data treatment of XPS spectra can be applied to understand the chemical composition of their very different surface layers. XPS data from model organisms as well as reference substances representing specific building blocks of their surface were collected and are presented. These results aims to guide future analysis of the surface chemical composition of biological systems.

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Section: Virus Surface Chemistrymentioning

confidence: 99%

Applying Cryo-X-ray Photoelectron Spectroscopy to Study the Surface Chemical Composition of Fungi and Viruses

et al. 2021

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“…The laser interaction with material in liquid medium yields direct synthesis of nanoparticles with distinct microstructures compared to other conventional synthesis techniques. ,,− The recent development of controlled laser ablation synthesis and resulting microstructure of pure metals, − alloys, − composites, − and biocompatible − nanoparticles show the diverse potential applications of this method.…”

Section: Introductionmentioning

confidence: 99%

Temperature- and Size-Dependent Compositionally Tuned Microstructural Landscape for Ag-46 Atom % Cu Nanoalloy Prepared by Laser Ablation in Liquid

Malviya

Chattopadhyay

2016

J. Phys. Chem. C

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We report a temperature- and size-dependent compositionally tuned microstructural landscape for Ag-46 atom % Cu alloy nanoparticles. The microstructural and morphological changes were established through the technique of in situ transmission electron microscopy. The nanoparticles were synthesized by laser ablation of alloy target in an aqueous medium. The as-synthesized particles predominantly contain nanosized grains of a single-phase solid solution with grains having sizes ∼3 ± 0.5 nm. For particles with smaller sizes (∼20 nm), the solid solution decomposes and grains coarsen on heating to yield predominantly bicrystals containing two phases of Ag-rich and Cu-rich solid solution. The microstructure of the larger particles (≥40 nm) evolves through segregation of Ag and their preferential growth near the surface of the particles. This leads to a core–shell-like composition distribution at a certain range of temperatures (≥200 °C) and sizes (≥32 nm). At higher temperatures, these core–shell particles undergo a morphological transition through grain growth yielding bicrystals of two phases. We present a size- and temperature-dependent morphology diagram that captures these changes.

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“…With the characteristics of distinct localized surface plasmon resonance (LSPR), gold nanoparticles (GNPs) and magnetic nanoparticles (MNPs) with sizes larger than 50 nm can scatter bright colored light under a common dark-field microscope, which allows the naked-eye observation of the detection event . With a high-resolution dark-field microscopy system, the multicolor of the scattered light of GNP can be directly photographed and has been applied in the detection of pathogen, biomarker molecules of Alzheimer and cancer cells via DNA-modified GNP. − For pathogenic bacteria detection, GNP has been employed to enable methicillin-resistant Staphylococcus aureus (MRSA) to be observed under dark-field microscope . We also successfully developed a MNP-based dark-field assay for the detection of micron-sized pathogens such as Escherichia coli (∼1.5 μm) and Cryptosporidium parvum (∼5 μm) by constructing a wreath-like structure with MNPs of diameters 50 and 800 nm, respectively. , However, the MNP probe at 50 nm or larger size is ineffective to be employed to form a wreath-like structure for the pathogen with a size of only ∼200 nm because we found that only a few MNP probes at 50 nm on average attached to one target pathogen after incubation with target pathogens for 30 min or even longer.…”

mentioning

confidence: 99%

“…26−28 For pathogenic bacteria detection, GNP has been employed to enable methicillin-resistant Staphylococcus aureus (MRSA) to be observed under dark-field microscope. 29 We also successfully developed a MNP-based dark-field assay for the detection of micron-sized pathogens such as Escherichia coli (∼1.5 μm) and Cryptosporidium parvum (∼5 μm) by constructing a wreathlike structure with MNPs of diameters 50 and 800 nm, respectively. 17,18 However, the MNP probe at 50 nm or larger size is ineffective to be employed to form a wreath-like structure for the pathogen with a size of only ∼200 nm because we found that only a few MNP probes at 50 nm on average attached to one target pathogen after incubation with target pathogens for 30 min or even longer.…”

mentioning

confidence: 99%

Naked-Eye Enumeration of Single Chlamydia pneumoniae Based on Light Scattering of Gold Nanoparticle Probe

Chen

Yang

et al. 2020

ACS Sens.

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Chlamydia pneumoniae is a spherical zoonotic pathogen with a diameter of ∼200 nm, which can lead to a wide range of acute and chronic diseases in human body. Early and reliable on-site detection of C. pneumoniae is the key step to control the spread of the pathogen. However, the lack of a current technology with advantages of rapidity, ultrasensitivity, and convenience limits the implementation of traditional techniques for on-site detection of C. pneumoniae. Herein, we developed a naked-eye counting of C. pneumoniae based on the light scattering properties of gold nanoparticle (GNP) under dark-field microscopy (termed "GNP-labeled dark-field counting strategy"). The recognition of single C. pneumoniae by anti-C. pneumoniae antibodies-functionalized GNP probes with size of 15 nm leads to the formation of wreath-like structure due to the strong scattered light resulted from hundreds of GNP probes binding on one C. pneumoniae under dark-field microscopy. Hundreds of GNP probes can bind to the surface of C. pneumoniae due to the high stability and specificity of the nucleic acid immuno-GNP probes, which generates by the hybridization of DNA-modified GNP with DNA-functionalized antibodies. The limit of detection (LOD) of the GNP-labeled darkfield counting strategy for C. pneumoniae detection in spiked samples or real samples is down to four C. pneumoniae per microliter, which is about 4 times more sensitive than that of quantitative polymerase chain reaction (qPCR). Together with the advantages of the strong light scattering characteristic of aggregated GNPs under dark-field microscopy and the specific identification of functionalized GNP probes, we can detect C. pneumoniae in less than 30 min using a cheap and portable microscope even if the sample contains only a few targets of interest and other species at high concentration. The GNP-labeled dark-field counting strategy meets the demands of rapid detection, low cost, easy to operate, and on-site detection, which paves the way for early and on-site detection of infectious pathogens.

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Surface Chemistry of Bacteriophage and Laser Ablated Nanoparticle Complexes for Pathogen Detection

Cited by 9 publications

References 56 publications

Applying Cryo-X-ray Photoelectron Spectroscopy to Study the Surface Chemical Composition of Fungi and Viruses

Applying Cryo-X-ray Photoelectron Spectroscopy to Study the Surface Chemical Composition of Fungi and Viruses

Temperature- and Size-Dependent Compositionally Tuned Microstructural Landscape for Ag-46 Atom % Cu Nanoalloy Prepared by Laser Ablation in Liquid

Naked-Eye Enumeration of Single Chlamydia pneumoniae Based on Light Scattering of Gold Nanoparticle Probe

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