Surface functionalization of porous polypropylene membranes with polyaniline for protein immobilization

Piletsky, Sergey A.; Piletska, Elena; Bossi, Alessandra Maria; Turner, Nicholas W.; Turner, Anthony

doi:10.1002/bit.10544

Cited by 52 publications

(41 citation statements)

References 20 publications

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“…243,623 Other surface-imprinting approaches have involved modified poly(aniline) films, 837 and imprinted layer grafted to silica surfaces 762,764 or to membranes. 838 The use of the 'epitope' approach [465][466][467]839 may be a promising solution to the imprinting of proteins, avoiding some of the difficulties of working with the entire protein. Bacterial cells were imprinted on beaded polymers 674 and films 676 using a 'lithographic' approach.…”

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confidence: 99%

Molecular imprinting science and technology: a survey of the literature for the years up to and including 2003

Andersson²,

et al. 2006

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Over 1450 references to original papers, reviews and monographs have herein been collected to document the development of molecular imprinting science and technology from the serendipitous discovery of Polyakov in 1931 to recent attempts to implement and understand the principles underlying the technique and its use in a range of application areas. In the presentation of the assembled references, a section presenting reviews and monographs covering the area is followed by papers dealing with fundamental aspects of molecular imprinting and the development of novel polymer formats. Thereafter, literature describing attempts to apply these polymeric materials to a range of application areas is presented.

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confidence: 99%

Molecular imprinting science and technology: a survey of the literature for the years up to and including 2003

Andersson²,

et al. 2006

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“…The dry state seems a better condition for the maintenance of the enzyme activity than the water environment, the latter being favourable for the hydrolysation of the covalent bonds. This statement is supported by observation of peroxidases immobilised on modified membranes, where the enzymes stored in the dry state at 4°C retained about 80% of their original activity over a period of 3 months [22]. In the present case, the shelf life of the CE-microreactor is a major advantage.…”

Section: Stability Of the Immobilised Enzyme In The Ce-microreactormentioning

confidence: 60%

Controlled enzyme-immobilisation on capillaries for microreactors for peptide mapping

et al. 2003

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In the present paper, the covalent immobilisation of the digesting enzyme trypsin has been achieved through photo-immobilisation on a portion of a silica capillary, thus leading to the construction of a capillary electrophoretic (CE)-microreactor for peptide mapping. The CE-microreactor is characterised by being a single piece, thus ensuring no fluidic or electrical leakage. The enzyme was immobilised with a surface density of 15.8 microg/cm(2), the stability was high (80% after 38 days) and the rate of conversion was 0.2 ng/s. On-line protein mapping was tested with proteins of different dimensions, showing competitiveness in terms of time (completed map within 15 min) and exhaustive maps of small proteins. The results of the CE-microreactor and the potential to immobilise biocomponents easily on a desired portion of the capillary indicate further developments towards the construction of a variety of miniaturised enzymatic screening devices for high-throughput screening analysis.

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“…However, controlling the interactions between the adsorbed molecule and the surface is Direct bioelectrocatalysis at the interfaces by genetically engineered dehydrogenase Yucesoy et al Yucesoy et al difficult due to the weak and the non-specific nature of the attachment process. [16][17][18] Chemical coupling that provides a more stable interfacial interaction is a widely used immobilisation strategy. [19][20][21] Self-assembled monolayers (SAMs) have been the indispensable approach to functionalise the metallic surfaces for attaching any type of biomolecules.…”

Section: Introductionmentioning

confidence: 99%

Direct bioelectrocatalysis at the interfaces by genetically engineered dehydrogenase

Yucesoy

Karaca

Çetinel³

et al. 2015

Bioinspired, Biomimetic and Nanobiomaterials

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There is an emerging interest in developing bio-functionalisation routes serving as platforms for assembling diverse enzymes onto material surfaces. Specifically, the fabrication of next-generation, laboratory-on-a-chip-based sensing and energy-harvesting systems requires controlled orientation and organisation of the proteins at the inorganic interfaces. Herein, the authors take the initial steps towards designing multifunctional, enzyme-based platforms by genetically integrating the engineered materialselective peptide tags for tethering redox enzymes onto electrode surfaces. The authors engineered a fusion protein that genetically conjugates gold-binding peptide to formate dehydrogenase derived from Candida methylica. The expressed proteins were tested for both enzyme activity and self-directed gold-surface functionalisation ability. Their findings demonstrate the successful self-immobilisation of the engineered enzyme onto different gold electrodes while retaining the catalytic activity.Building on the functionalisation by the peptides, a fusion enzyme-integrated circuit-based biosensor system was designed. The catalytic conversion of the formate by the engineered dehydrogenase was successfully monitored on the electrode surface at subsequent intervals. The engineered peptide-mediated self-integrated electrode systems can be extended to develop a wide range of biosensing and energy-harvesting platforms using different combinations of materials and biomolecules. This paper contains supporting information that will be made available online once the issue is published. In the meantime, if you wish to get a copy of the supplementary file, please contact the Journals Editor, Sarah Brown, at sarah.brown@icepublishing.com.

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Surface functionalization of porous polypropylene membranes with polyaniline for protein immobilization

Cited by 52 publications

References 20 publications

Molecular imprinting science and technology: a survey of the literature for the years up to and including 2003

Molecular imprinting science and technology: a survey of the literature for the years up to and including 2003

Controlled enzyme-immobilisation on capillaries for microreactors for peptide mapping

Direct bioelectrocatalysis at the interfaces by genetically engineered dehydrogenase

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