Surface immobilizable chelator for label-free electrical detection of pyrophosphate

Liu, Jianquan; Credo, Grace M.; Su, Xing; Wu, Kai; Lim, Hsiao C.; Elibol, Oğuz H.; Bashir, Rashid; Varma, Madoo

doi:10.1039/c1cc12073e

Cited by 25 publications

(25 citation statements)

References 16 publications

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“…In addition to ellipsometry, the expected mass of the chelator modification on silicon substrates has been detected by surface-sensitive TOF-SIMS. 5 …”

Section: Resultsmentioning

confidence: 99%

“…The synthesis and storage of the amine-functionalized chelator designed for PPi capture was described in a separate publication from our group. 5 Buffers used in these procedures included 1x phosphate buffered saline (PBS) at pH 8, 0.1 M 2-( N -morpholino)ethanesulfonic acid (MES) buffer at pH 5, and 0.2 M sodium borate buffer at pH 8. For rinsing non-specifically bound components such as avidin and storage of DNA colonies attached to device surfaces, we typically used a custom 1x ‘TEST’ (Tris, EDTA, Salt, Triton) buffer with composition 10 mM TrisHCl at pH 8, 1 mM EDTA, 50 mM NaCl and 0.01% Triton X-100.…”

Section: Methodsmentioning

confidence: 99%

“…We have previously reported the synthesis of a surface-immobilizable chelator based on di-(2-picolyl) amine (DPA) which has demonstrated strong binding affinity to PPi and demonstrated reversible SOI-FET sensing of PPi standard solutions. 5 …”

Section: Introductionmentioning

confidence: 99%

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Label-free electrical detection of pyrophosphate generated from DNA polymerase reactions on field-effect devices

Credo

et al. 2012

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We introduce a label-free approach for sensing polymerase reactions on deoxyribonucleic acid (DNA) using a chelator-modified silicon-on-insulator field-effect transistor (SOI-FET) that exhibits selective and reversible electrical response to pyrophosphate anions. The chemical modification of the sensor surface was designed to include rolling-circle amplification (RCA) DNA colonies for locally enhanced pyrophosphate (PPi) signal generation and sensors with immobilized chelators for capture and surface-sensitive detection of diffusible reaction by-products. While detecting arrays of enzymatic base incorporation reactions is typically accomplished using optical fluorescence or chemiluminescence techniques, our results suggest that it is possible to develop scalable and portable PPi-specific sensors and platforms for broad biomedical applications such as DNA sequencing and microbe detection using surface-sensitive electrical readout techniques.

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“…In addition to ellipsometry, the expected mass of the chelator modification on silicon substrates has been detected by surface-sensitive TOF-SIMS. 5 …”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Label-free electrical detection of pyrophosphate generated from DNA polymerase reactions on field-effect devices

Credo

et al. 2012

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“…Further advances of these technologies require the ability to integrate additional elements, such as the miniaturized heating element described here, and the ability to integrate heating elements in a massively parallel format compatible with silicon technology (26). Notably, our miniaturized heaters could also function as dual heater/sensor elements, as these SOI nanowire or nanoribbon structures have been used to detect DNA, proteins, pH, and pyrophosphates (27)(28)(29)(30)(31).…”

Section: Discussionmentioning

confidence: 99%

Ultralocalized thermal reactions in subnanoliter droplets-in-air

Salm

Guevara

Dak

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Miniaturized laboratory-on-chip systems promise rapid, sensitive, and multiplexed detection of biological samples for medical diagnostics, drug discovery, and high-throughput screening. Within miniaturized laboratory-on-chips, static and dynamic droplets of fluids in different immiscible media have been used as individual vessels to perform biochemical reactions and confine the products. Approaches to perform localized heating of these individual subnanoliter droplets can allow for new applications that require parallel, time-, and spacemultiplex reactions on a single integrated circuit. Our method positions droplets on an array of individual silicon microwave heaters on chip to precisely control the temperature of droplets-in-air, allowing us to perform biochemical reactions, including DNA melting and detection of single base mismatches. We also demonstrate that ssDNA probe molecules can be placed on heaters in solution, dried, and then rehydrated by ssDNA target molecules in droplets for hybridization and detection. This platform enables many applications in droplets including hybridization of low copy number DNA molecules, lysing of single cells, interrogation of ligand-receptor interactions, and rapid temperature cycling for amplification of DNA molecules.nanowire | on-chip heating | evaporation

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“…1) so that, bound to an ISFET surface, pyrophosphate production can be read out as a signal of nucleotide addition. 10 …”

Section: Dna Sequencing With Isfetsmentioning

confidence: 99%

Biochemistry and semiconductor electronics—the next big hit for silicon?

Lindsay

2012

J. Phys.: Condens. Matter

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Two recent developments portend a new era for silicon electronics in biomedical applications. Firstly, highly specific chemical recognition and massively parallel sample preparation techniques are being combined with VLSI to make new kinds of analytical chips. Secondly, critical dimensions are beginning to approach the size of biomolecules, opening new pathways for physical interactions between molecules and semiconductor structures. Future generations of hybrid chemical-CMOS devices could revolutionize diagnosis and make personalized medicine cheap enough to become widespread.

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Surface immobilizable chelator for label-free electrical detection of pyrophosphate

Cited by 25 publications

References 16 publications

Label-free electrical detection of pyrophosphate generated from DNA polymerase reactions on field-effect devices

Label-free electrical detection of pyrophosphate generated from DNA polymerase reactions on field-effect devices

Ultralocalized thermal reactions in subnanoliter droplets-in-air

Biochemistry and semiconductor electronics—the next big hit for silicon?

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