Surface markers: An identity card of endothelial cells

Ribatti, Doménico; Tamma, Roberto; Ruggieri, Simona; Annese, Tiziana; Crivellato, Enrico

doi:10.1111/micc.12587

Cited by 30 publications

(21 citation statements)

References 93 publications

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“…Previously, lipofibroblasts have been reported to be involved in alveolar development, regeneration, surfactant synthesis and vitamin A (retinoic acid) storage [33], and contribute to the alveolar epithelial cell II (AECII) stem cell niche in the adult mouse lung [34]. The enrichment result in endothelial cells was mainly related to vasculature development ( Figure 5E), which is consistent with the knowledge of endothelial cells as vasculature forming cells [35]. Several varied terms were identified among the enriched biological processes of Pdgfrb high (Pdgfrb hi) cells, suggesting that this cell type is a transitional cell type.…”

Section: Go Enrichment Analysis Identifies Different Roles Of the Eigsupporting

confidence: 89%

Deconvolution of RNA-Seq Analysis of Hyperbaric Oxygen-Treated Mice Lungs Reveals Mesenchymal Cell Subtype Changes

Yuan

Zhou

et al. 2020

IJMS

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Hyperbaric oxygen (HBO) is widely applied to treat several hypoxia-related diseases. Previous studies have focused on the immediate effect of HBO-exposure induced oxidative stress on the lungs, but knowledge regarding the chronic effects from repetitive HBO exposure is limited, especially at the gene expression level. We found that repetitive HBO exposure did not alter the morphology of murine lungs. However, by deconvolution of RNA-seq from those mice lungs using CIBERSORTx and the expression profile matrices of 8 mesenchymal cell subtypes obtained from bleomycin-treated mouse lungs, we identify several mesenchymal cell subtype changes. These include increases in Col13a1 matrix fibroblasts, mesenchymal progenitors and mesothelial cell populations and decreases in lipofibroblasts, endothelial and Pdgfrb high cell populations. Our data suggest that repetitive HBO exposure may affect biological processes in the lungs such as response to wounding, extracellular matrix, vasculature development and immune response.

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Section: Go Enrichment Analysis Identifies Different Roles Of the Eigsupporting

confidence: 89%

Deconvolution of RNA-Seq Analysis of Hyperbaric Oxygen-Treated Mice Lungs Reveals Mesenchymal Cell Subtype Changes

Yuan

Zhou

et al. 2020

IJMS

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“…The gradual decline in expression of genes along with the projection proved that ECs are exhibiting a continuous phenotype, coined zonation (Vanlandewijck et al, 2018), rather than showing discreet phenotypes. Other patterns of markedly expressed genes that can be found in arteries are Ephrin B2, Alk1, Dll4, NRP1, Depp, Hey1 and Hey2, EPAS1 while veins express a pattern of Ephrin B2, Eph B4, NRP2, and COUP−TFII (Cheng et al, 2002;Aird, 2007;Ribatti et al, 2020).…”

Section: Intra-tissue Heterogeneity/heterogeneity Within the Vascularmentioning

confidence: 99%

“…In an effort to study heterogeneity within PCs, Vanlandewijck et al (2018) identified PCs among other cell types within murine brain samples by applying known PC gene patterns to their data set. Sorting for the presence of canonical PC markers such as Pdgfrb, Cspg4, and Des and excluding cells expressing smooth muscle cell (SMC) markers like Acta2 and TagIn, as well as fibroblast markers Cheng et al, 2002;Aird, 2007;Ribatti et al, 2020 Arteries Alk1 Cell-surface receptor Mouse Cheng et al, 2002;Aird, 2007;Ribatti et al, 2020 Arteries Dll4 Notch ligand Mouse Cheng et al, 2002;Aird, 2007;Ribatti et al, 2020 Arteries Nrp1 Neuropilin 1 Mouse Cheng et al, 2002;Aird, 2007;Ribatti et al, 2020 Arteries Depp Decidual protein induced by progesterone Mouse Cheng et al, 2002;Aird, 2007;Ribatti et al, 2020 Arteries Hey1 Transcription factor Mouse Cheng et al, 2002;Aird, 2007;Ribatti et al, 2020 Arteries Hey2 Transcription factor Mouse Cheng et al, 2002;Aird, 2007;Ribatti et al, 2020 Arteries…”

Section: Heterogeneity In Pericytesmentioning

confidence: 99%

“…Endothelial PAS domain-containing protein 1 Mouse Cheng et al, 2002;Aird, 2007;Ribatti et al, 2020 Capillaries, veins Cheng et al, 2002;Aird, 2007;Ribatti et al, 2020 Veins…”

Section: Epas1mentioning

confidence: 99%

“…Ephrin type-B receptor 4 Mouse Cheng et al, 2002;Aird, 2007;Ribatti et al, 2020 Veins Nrp2 Neuropilin 2 Mouse Cheng et al, 2002;Aird, 2007;Ribatti et al, 2020 FIGURE 3 | Vascular heterogeneity between sections of the vascular tree: schematic depiction of genes differentially upregulated in endothelial cells in the different sections of the vascular tree.…”

Section: Ephb4mentioning

confidence: 99%

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Vascular Heterogeneity With a Special Focus on the Hepatic Microenvironment

et al. 2020

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Utilizing single-cell sequencing, recent studies were able to analyze at a greater resolution the heterogeneity of the vasculature and its complex composition in different tissues. Differing subpopulations have been detected, distinguishable only by their transcriptome. Designed to provide further insight into the heterogeneity of the functional vascular tissue, endothelial cells have been the main target of those studies. This review aims to present a synopsis of the variability of the different vascular beds, their endothelial variety, and the supporting cells that allow the vessels to serve their various purposes. Firstly, we are going to chart vascular tissue heterogeneity on a cellular level, describing endothelial diversity as well as stromal microenvironmental variety and interaction in a physiological setting. Secondly, we will summarize the current knowledge of pathological vessel formation in the context of cancer. Conventional anti-tumor therapeutic targets as well as anti-angiogenetic therapy is frequently limited by poor response of the tumor tissue. Reasons for moderate response and resistance to treatment can be found through different drivers of angiogenesis, different mechanisms of blood supply, but also in poorly understood tissue diversity. Based on this, we are comparing how pathologies alter the normal structure of vascular tissues highlighting the involved mechanisms. Lastly, illustrating the concept above, we will focus on the hepatic microenvironment, an organ of frequent metastatic spreading (e.g., from colorectal, breast, and lung cancers). We will address how the hepatic vasculature usually develops and subsequently we will describe how common liver metastases vary in their vasculature and the way they supply themselves (e.g., angiogenesis versus vessel co-option).

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VEGF‐delivering PEG hydrogels promote vascularization in the porcine subcutaneous space

Quizon,

Deppen,

Barber

et al. 2024

J Biomedical Materials Res

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For cell therapies, the subcutaneous space is an attractive transplant site due to its large surface area and accessibility for implantation, monitoring, biopsy, and retrieval. However, its poor vascularization has catalyzed research to induce blood vessel formation within the site to enhance cell revascularization and survival. Most studies focus on the subcutaneous space of rodents, which does not recapitulate important anatomical features and vascularization responses of humans. Herein, we evaluate biomaterial‐driven vascularization in the porcine subcutaneous space. Additionally, we report the first use of cost‐effective fluorescent microspheres to quantify perfusion in the porcine subcutaneous space. We investigate the vascularization‐inducing efficacy of vascular endothelial growth factor (VEGF)‐delivering synthetic hydrogels based on 4‐arm poly(ethylene) glycol macromers with terminal maleimides (PEG‐4MAL). We compare three groups: a non‐degradable hydrogel with a VEGF‐releasing PEG‐4MAL gel coating (Core+VEGF gel); an uncoated, non‐degradable hydrogel (Core‐only); and naïve tissue. After 2 weeks, Core+VEGF gel has significantly higher tissue perfusion, blood vessel area, blood vessel density, and number of vessels compared to both Core‐only and naïve tissue. Furthermore, healthy vital signs during surgery and post‐procedure metrics demonstrate the safety of hydrogel delivery. We demonstrate that VEGF‐delivering synthetic hydrogels induce robust vascularization and perfusion in the porcine subcutaneous space.

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Surface markers: An identity card of endothelial cells

Cited by 30 publications

References 93 publications

Deconvolution of RNA-Seq Analysis of Hyperbaric Oxygen-Treated Mice Lungs Reveals Mesenchymal Cell Subtype Changes

Deconvolution of RNA-Seq Analysis of Hyperbaric Oxygen-Treated Mice Lungs Reveals Mesenchymal Cell Subtype Changes

Vascular Heterogeneity With a Special Focus on the Hepatic Microenvironment

VEGF‐delivering PEG hydrogels promote vascularization in the porcine subcutaneous space

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