Surface microbiota and associated staphylococci of houseflies (Musca domestica) collected from different environmental sources

Sudağıdan, Mert; Özalp, Veli Cengiz; Can, Özge; Eligül, Hakan; Yurt, Mediha Nur Zafer; Taşbaşi, Behiye Büşra; Acar, Elif Esma; Kavruk, Murat; Koçak, Öner

doi:10.1016/j.micpath.2022.105439

Cited by 8 publications

(10 citation statements)

References 40 publications

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“…Sobur and coworkers found a high percentage of S. aureus resistant to amoxicillin and oxacillin in house flies collected near a human hospital; most strains were susceptible to ciprofloxacin and gentamicin [ 27 ]. In a recent study on CoNS isolated from house flies collected from different environments, a high percentage of tested staphylococci were resistant to oxacillin and penicillin but susceptible to tetracycline, ciprofloxacin, gentamicin, and chloramphenicol [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistant Coagulase-Negative Staphylococci Carried by House Flies (Musca domestica) Captured in Swine and Poultry Farms

et al. 2023

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House flies (Musca domestica) are very diffuse insects attracted by biological materials. They are abundantly present in farm environments and can frequently come in contact with animals, feed, manure, waste, surfaces, and fomites; consequently, these insects could be contaminated, carry, and disperse several microorganisms. The aim of this work was to evaluate the presence of antimicrobial-resistant staphylococci in house flies collected in poultry and swine farms. Thirty-five traps were placed in twenty-two farms; from each trap, 3 different kinds of samples were tested: attractant material present in the traps, the body surface of house flies and the body content of house flies. Staphylococci were detected in 72.72% of farms, 65.71% of traps and 43.81% of samples. Only coagulase-negative staphylococci (CoNS) were isolated, and 49 isolates were subjected to an antimicrobial susceptibility test. Most of the isolates were resistant to amikacin (65.31%), ampicillin (46.94%), rifampicin (44.90%), tetracycline (40.82%) and cefoxitin (40.82%). Minimum Inhibitory concentration assay allowed to confirm 11/49 (22.45%) staphylococci as methicillin-resistant; 4 of them (36.36%) carried the mecA gene. Furthermore, 53.06% of the isolates were classified as multidrug-resistant (MDR). Higher levels of resistance and multidrug resistance were detected in CoNS isolated from flies collected in poultry farms than in swine farms. Therefore, house flies could carry MDR and methicillin-resistant staphylococci, representing a possible source of infection for animals and humans.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistant Coagulase-Negative Staphylococci Carried by House Flies (Musca domestica) Captured in Swine and Poultry Farms

et al. 2023

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“…[ 49 , 50 , 53 , 54 , 55 , 56 ], Aeromonas cavie [ 57 ], and bacterial pathogens causing bovine respiratory disease Mannheimia haemolytica , Pasteurella multocida , Histophilus somni [ 58 ]. Culture-based and more recently (i.e., within the past 20 years) molecular-based surveys have provided even more extensive lists of pathogens carried by flies, which have already been well summarized in other studies or reviews [ 14 , 15 , 16 , 17 , 18 , 59 , 60 , 61 , 62 , 63 , 64 ] and will not be recapitulated here. As carriers of many pathogenic bacteria, adult house flies are undoubtedly key players in food security, specifically in pre- and post-harvest food safety across a variety of food animal species [ 42 , 65 , 66 ].…”

Section: Wild House Flies Are Potential Threats To Animal and Human H...mentioning

confidence: 99%

“…House flies collected from animal production facilities ( Table S1 ; [ 10 , 22 , 43 , 45 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 ] and urban environments [ 10 , 63 , 84 ] exhibit a wide diversity of AMR bacteria including potential human and animal pathogenic species. Multi-drug-resistant bacteria have also been isolated from these flies including Staphylococcus spp.…”

Section: House Flies As Reservoirs and Disseminators Of Antimicrobial...mentioning

confidence: 99%

“…Multi-drug-resistant bacteria have also been isolated from these flies including Staphylococcus spp. isolated with multiple antibiotic-resistant genes (ARGs; msrA , msrB , tetK , tetM , tetL , ermC , and aad6 ) exhibiting multidrug resistance (oxacillin, penicillin, erythromycin, clindamycin, tetracycline, ciprofloxacin, gentamicin, and chloramphenicol) phenotype [ 63 ]. Providencia spp.…”

Section: House Flies As Reservoirs and Disseminators Of Antimicrobial...mentioning

confidence: 99%

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House Flies Are Underappreciated Yet Important Reservoirs and Vectors of Microbial Threats to Animal and Human Health

et al. 2023

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House flies are well recognized as filth-associated organisms and public nuisances. House flies create sanitation issues when they bridge the gap between microbe-rich breeding environments and animal/human habitations. Numerous scientific surveys have demonstrated that house flies harbor bacterial pathogens that pose a threat to humans and animals. More extensive and informative surveys incorporating next-generation sequencing technologies have shown that house fly carriage of pathogens and harmful genetic elements, such as antimicrobial resistance genes, is more widespread and dangerous than previously thought. Further, there is a strong body of research confirming that flies not only harbor but also transmit viable, and presumably infectious, bacterial pathogens. Some pathogens replicate and persist in the fly, permitting prolonged shedding and dissemination. Finally, although the drivers still have yet to be firmly determined, the potential range of dissemination of flies and their associated pathogens can be extensive. Despite this evidence, the house flies’ role as reservoirs, disseminators, and true, yet facultative, vectors for pathogens have been greatly underestimated and underappreciated. In this review, we present key studies that bolster the house fly’s role both an important player in microbial ecology and population biology and as transmitters of microbial threats to animal and human health.

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“…Штаммы были в основном устойчивы к оксациллину, пенициллину и эритромицину, а три штамма обладали множественной лекарственной устойчивостью. В ходе этого исследования были изучены поверхностная микробиота и носительство патогенных стафилококков на поверхности тела комнатных мух [2].…”

Section: постановка проблемы (Introduction)unclassified

Population density analysis of Diptera: Muscidae in livestock objects

Reshetnikov

Barashkova

Budischeva

2022

Agrarian Bulletin of The

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Abstract. Pig breeding is inevitably accompanied by an attack on animals by a large number of flies. Effective fly control requires biological information about the dynamics of the fly population over the seasons. The aim of the study was to determine the number and species composition of flies in the conditions of the Khatas pig farm in the winter. Methods. In the Khatas pig farm, during the technological cycle of production in December 2021, studies were carried out to determine the number of flies. To count the number of flies in the pigsties of the breeding, reproduction and fattening shops, traps were installed in the form of sticky tapes 84 cm long. There were 15 pigsties in three workshops of the pig farm. In each pigsty, one trap was placed at a height of 1.5 m from the floor. The number of insects caught on the glue trap was counted after 24 hours under laboratory conditions. Data analysis was carried out according to the method of V. N. Beklemishev. A trap with an exposure of 24 hours was taken as the unit for counting the number of flies. Results. For the first time, research has established the spread of flies in winter during the technological cycle of production at Khatas pig farm LLC. During the day, 1102 flies were caught by traps in three workshops. The species composition of flies in winter was represented by one species of Musca domestica L .The abundance index of adult flies in the rearing shop was 30.25 ± 7.56 flies per trap-day, and in the reproduction and fattening shops – 34 ± 5.78, and 57.2 ± 8.55 flies per trap-day, respectively. The scientific novelty lies in the fact that the number of adults of flies and their species composition in the Khatas pig farm of Yakutia in the winter period were determined for the first time in the obtained materials.

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Surface microbiota and associated staphylococci of houseflies (Musca domestica) collected from different environmental sources

Cited by 8 publications

References 40 publications

Antimicrobial Resistant Coagulase-Negative Staphylococci Carried by House Flies (Musca domestica) Captured in Swine and Poultry Farms

Antimicrobial Resistant Coagulase-Negative Staphylococci Carried by House Flies (Musca domestica) Captured in Swine and Poultry Farms

House Flies Are Underappreciated Yet Important Reservoirs and Vectors of Microbial Threats to Animal and Human Health

Population density analysis of Diptera: Muscidae in livestock objects

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