Surface Molecular Components of Human Lymphocytes

Mehrishi, J.N.

doi:10.1159/000230593

Cited by 12 publications

(8 citation statements)

References 19 publications

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“…This is supported indirectly by the evidence that the electrokinetic behavior of lymphocytes from the various organs in non-human primates closely resembles that of rodents [3]. It may be recafled that in previous studies [8,9] human peripheral blood lymphocytes had been isolated by the carbonyl iron/methyl cellulose method [34] and the cells had not been obtained in distinct cell populations. It is interesting to note that the EPM of such lymphocytes (-1.09 cellulose method) 1341.…”

Section: Resultsmentioning

confidence: 93%

“…Furthermore, it must be considered that by the chemical modifications used, only susceptible groups can be detected, whereas the number and exact character of nonsusceptible groups that contribute to the surface charge remain undefined. For significance and specificity of the chemical treatment for detection of surface groups see references [8][9][10][16][17][18][19][20][21].…”

Section: Resultsmentioning

confidence: 99%

“…The data on human peripheral blood lymphocytes [ 8,9] are given only for comparison with that on murine lymphocytes (Table 1, Fig. 2).…”

Section: Resultsmentioning

confidence: 99%

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Surface molecular components of T and B lymphocytes

Mehrishi

Zeiller

1974

Eur J Immunol

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Biochemical and biophysical methods have demonstrated differences in the surface topochemistry of murine T and B lymphocytes. On the surface of B cells, negatively charged groups of sialic acid, phosphate groups and unidentified acidic groups, positively charged amino groups and sulfhydryl groups are present. On the surface of T cells, which carry a higher net negative charge than B cells, the presence of phosphate and sulfhydryl groups has not been detected; the number of the positively charged groups is much smaller and that of carboxyl groups of sialic acid much larger than those on the B cell.

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

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Surface molecular components of T and B lymphocytes

Mehrishi

Zeiller

1974

Eur J Immunol

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“…Conseqtiently, a live intact cell in active metabolic state exhibits a characteristic surface electrical charge and electrophoretic mobility. The cellular EPM has never been found to be zero for cells examined so far, and its relevance in biology and medicine is now generally accepted [27,30,35,40 and references to Curtis & Weiss therein].…”

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confidence: 99%

Age‐dependent Changes in the Electrophoretic Mobilities of Human Blood Lymphocytes

Wioland¹,

Mehrishi²

1979

Scand J Immunol

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The distribution of the anodic electrophoretic mobilities (EPM) of human peripheral blood lymphocytes was determined for lymphocytes isolated from umbilical cord blood and from blood of individuals 6 months to 93 years of age. The distribution was bimodal in infants up to 2 years of age and suggested a small percentage of cells with a mobility of 0.95 micrometer s-1 V-1 cm. this value was chosen to discriminate between low-mobility cells (LMCs) and high-mobility cells (HMCs). The relative percentage of LMCs increased from birth to 2 years and two types of LMCs could be distinguished. The distribution was unimodal and asymmetric in children and adults and nearly Gaussian in aged people. Substantial differences between the distributions of the lymphocyte EPMs were seen on comparison of the histograms for individuals of similar ages. The analysis of the distribution of the lymphocyte EPMs on cell suspensions enriched in, or depleted of T or B cells confirmed the mobility of most T cells to be higher than the mobility of most B cells, whatever the age of the individual. The distribution of lymphocyte EPMs determined in the same adult over a 6 year period showed minor variations.

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“…If rejection involves "recognition," both should be modified by "electrical masking," (4,6,7) that is by modification or rearrangement of the surface charges in the donor tissues, thus preventing antigen-antibody recognition and/or recipient recognition cells principally lymphocytes from attaching to the surface prosthetic groups of the organ transplant (8)(9)(10)(11)(12).…”

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confidence: 99%

Donor Recipient Lymphocyte Recognition in Kidney Transplantation: Electrochemical Techniques for Evaluating Donor Recipient Tissue Compatibility

Sawyer

Stanczewski

Ramasamy

et al. 1976

J. Electrochem. Soc.

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Classical methods based on cell recognition in vitro do not yield full information on tissue‐host compatibility. Acute rejection episodes are thus common in patients with transplanted kidneys. Determination of surface charge of lymphocytes has been employed to provide information on cell recognition. The results from 40 sets of matched kidney donors and recipients indicate that cell electrophoresis could be used to determine tissue‐host compatibility. Acute rejection occurs in cases where the electrophoretic mobility of mixed lymphocyte false(EMnormalmixfalse) is much higher or lower than that of the recipient false(EMRfalse) . If EMnormalmix is only marginally lower than EMR (±5%) the transplanted organ rejection rate is markedly reduced.

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Surface Molecular Components of Human Lymphocytes

Cited by 12 publications

References 19 publications

Surface molecular components of T and B lymphocytes

Surface molecular components of T and B lymphocytes

Age‐dependent Changes in the Electrophoretic Mobilities of Human Blood Lymphocytes

Donor Recipient Lymphocyte Recognition in Kidney Transplantation: Electrochemical Techniques for Evaluating Donor Recipient Tissue Compatibility

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