Surface plasmon resonance assay for exosomes based on aptamer recognition and polydopamine-functionalized gold nanoparticles for signal amplification

Liao, Guofu; Liu, Xiaofeng; Yang, Xiaohai; Wang, Qing; Geng, Xiuhua; Zou, Liyuan; Liu, Yaqin; Li, Shaoyuan; Zheng, Yan; Wang, Kemin

doi:10.1007/s00604-020-4183-1

Cited by 38 publications

(28 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This strategy allowed an LOD of 5 × 10 3 exosomes/mL, providing an avenue to capture exosomes. The same group recently introduced another amplification strategy using polydopamine-functionalized AuNPs (Figure 7b) [112]. Chloroauric acid (HAuCl 4 ) was reduced by polydopamine molecules to generate small AuNPs on the polydopaminemodified AuNPs, resulting in a further enhanced SPR response.…”

Section: Nanomaterial-based Sandwich Formatmentioning

confidence: 99%

See 1 more Smart Citation

Recent Advancements in Aptamer-Based Surface Plasmon Resonance Biosensing Strategies

Chang

2021

Biosensors

View full text Add to dashboard Cite

Surface plasmon resonance (SPR) can track molecular interactions in real time, and is a powerful as well as widely used biological and chemical sensing technique. Among the different SPR-based sensing applications, aptamer-based SPR biosensors have attracted significant attention because of their simplicity, feasibility, and low cost for target detection. Continuous developments in SPR aptasensing research have led to the emergence of abundant technical and design concepts. To understand the recent advances in SPR for biosensing, this paper reviews SPR-based research from the last seven years based on different sensing-type strategies and sub-directions. The characteristics of various SPR-based applications are introduced. We hope that this review will guide the development of SPR aptamer sensors for healthcare.

show abstract

Section: Nanomaterial-based Sandwich Formatmentioning

confidence: 99%

“…Aptamer-based SPR sensor for the determination of exosomes with (a) dual AuNPs and (b) polydopaminefunctionalized AuNPs for signal amplification. Reproduced with permission from[111] and[112]. Elsevier B.V., 2019.…”

mentioning

confidence: 99%

Recent Advancements in Aptamer-Based Surface Plasmon Resonance Biosensing Strategies

Chang

2021

Biosensors

View full text Add to dashboard Cite

show abstract

“…For increasing the specificity, they further developed a dual-aptamers-based SPR strategy for the determination of human hepatic carcinoma (SMMC-7721) exosomes. 152 In addition, combining with DNA-based signal amplification, Ding's group reported a hydrogel-AuNP supramolecular sphere (H-Au)-based SPR biosensor for analysis of prostate cancer-derived exosomes. 153 The H-Au network was prepared via the self-assembly of DNA chains and DNA-modified AuNPs.…”

Section: Surface Plasmon Resonance Biosensorsmentioning

confidence: 99%

Progress in Nanomaterials-Based Optical and Electrochemical Methods for the Assays of Exosomes

Ma¹,

Hao²,

Liu³

2021

IJN

View full text Add to dashboard Cite

“…Moreover, number of SPR platforms, including the Biacore, are configured with microfluidics, which makes them prone to clogging when using crude samples (Glynn et al., 2008). This configuration also restricts the usage of gold nanoparticles (AuNPs) for further signal amplification and improved sensitivity, a strategy recently proven useful on other non‐microfluidic SPR platform (Liao et al., 2020). Several advanced micro‐technology‐based plasmonic sensing and imaging strategies were reported also to enable selective EV detection along with improved LOD up to ∼10 5 EV/ml (Im et al., 2014) and multiplex capacity (Raghu et al., 2018; Thakur et al., 2017; Zhu et al., 2014b).…”

Section: Introductionmentioning

confidence: 99%

FO‐SPR biosensor calibrated with recombinant extracellular vesicles enables specific and sensitive detection directly in complex matrices

Yıldızhan

Vajrala

Geeurickx

et al. 2021

J of Extracellular Vesicle

View full text Add to dashboard Cite

Extracellular vesicles (EVs) have drawn huge attention for diagnosing myriad of diseases, including cancer. However, the EV detection and analyses procedures often lack much desired sample standardization. To address this, we used well‐characterized recombinant EVs (rEVs) for the first time as a biological reference material in developing a fiber optic surface plasmon resonance (FO‐SPR) bioassay. In this context, EV binding on the FO‐SPR probes was achieved only with EV‐specific antibodies (e.g. anti‐CD9 and anti‐CD63) but not with non‐specific anti‐IgG. To increase detection sensitivity, we tested six different combinations of EV‐specific antibodies in a sandwich bioassay. Calibration curves were generated with two most effective combinations (anti‐CD9/ B anti‐CD81 and anti‐CD63/ B anti‐CD9), resulting in 10 3 and 10 4 times higher sensitivity than the EV concentration in human blood plasma from healthy or cancer patients, respectively. Additionally, by using anti‐CD63/ B anti‐CD9, we detected rEVs spiked in cell culture medium and HEK293 endogenous EVs in the same matrix without any prior EV purification or enrichment. Lastly, we selectively captured breast cancer cell EVs spiked in blood plasma using anti‐EpCAM antibody on the FO‐SPR surface. The obtained results combined with FO‐SPR real‐time monitoring, fast response time and ease of operation, demonstrate its outstanding potential for EV quantification and analysis.

show abstract

Surface plasmon resonance assay for exosomes based on aptamer recognition and polydopamine-functionalized gold nanoparticles for signal amplification

Cited by 38 publications

References 32 publications

Recent Advancements in Aptamer-Based Surface Plasmon Resonance Biosensing Strategies

Recent Advancements in Aptamer-Based Surface Plasmon Resonance Biosensing Strategies

Progress in Nanomaterials-Based Optical and Electrochemical Methods for the Assays of Exosomes

FO‐SPR biosensor calibrated with recombinant extracellular vesicles enables specific and sensitive detection directly in complex matrices

Contact Info

Product

Resources

About