Surface Plasmon Resonance–Based Immunoassays

Moran, Kara; Lemass, Darragh; O’Kennedy, Richard

doi:10.1016/b978-0-12-811762-0.00006-2

Cited by 10 publications

(6 citation statements)

References 59 publications

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“…[ 33 ] Hence, the technique is sensitive to any change occurring within the first 300–400 nm of the gold facing the dielectric. [ 34 ] Here the local refractive index variations are correlated with the biomolecule interactions that can be inspected. The scanning of the SPR resonance angle allows the real‐time monitoring of each specimen approaching the metal surface from the dielectric medium.…”

Section: Resultsmentioning

confidence: 99%

Controlling the Binding Efficiency of Surface Confined Antibodies through the Design of Mixed Self‐Assembled Monolayers

Sarcina

Delre

Graziano

et al. 2023

Adv Materials Inter

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A plethora of different electronic and optoelectronic devices have been developed lately, for biosensing applications (e.g., label‐free, fast, and easier to operate) based on a detecting interface accommodating the biorecognition elements, anchored by thiolate self‐assembled monolayers (SAMs) on a gold surface. Here, a surface plasmon resonance (SPR) characterization of anti‐p24 anchored on different SAMs is performed to investigate the effect of the SAM structure on the antibodies’ packing efficiency and the sensors’ analytical figures of merit. Notably, the mixed SAM deposited from a solution 10:1 of 3‐mercaptopropionic acid and 11‐mercaptoundecanoic acid (11MUA) is compared to that resulting from a solution 10:1 of ad hoc synthesized N‐(2‐hydroxyethyl)‐3‐mercaptopropanamide (NMPA)/11MUA. Despite the improvement in the anti‐p24 surface coverage registered using the 11MUA/NMPA SAM, the latter produces a significant decrease in the antibodies’ binding efficiency against human immunodeficiency virus p24 protein. To provide a molecular rationale behind the SPR data, density functional theory calculations are also undertaken. A comprehensive physical view of the main competing phenomena affecting the biorecognition events at a biofunctionalized gold detecting interface is represented here.

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Section: Resultsmentioning

confidence: 99%

Controlling the Binding Efficiency of Surface Confined Antibodies through the Design of Mixed Self‐Assembled Monolayers

Sarcina

Delre

Graziano

et al. 2023

Adv Materials Inter

View full text Add to dashboard Cite

show abstract

“…Surface plasmon resonance (SPR) can be defined as charge-density oscillation at the interface when light passes through a substrate and is reflected by the metal-dielectric interface [ 255 ]. Sensor-based SPR is a label-free detection method that provides a reliable platform for the highly specific detection of various analytes, such as pollutants, antibiotics, biomolecules, pesticides, insecticides, herbicides, microorganisms, and microbial toxins [ 256 , 257 ]. Nanomaterials were utilized to employ smart layers of the SPR system, which orient the immobilization of the bio-receptors.…”

Section: Other Nanomaterial-based Methodologymentioning

confidence: 99%

Recent Progress in Nanotechnology-Based Approaches for Food Monitoring

et al. 2022

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Throughout the food supply chain, including production, storage, and distribution, food can be contaminated by harmful chemicals and microorganisms, resulting in a severe threat to human health. In recent years, the rapid advancement and development of nanotechnology proposed revolutionary solutions to solve several problems in scientific and industrial areas, including food monitoring. Nanotechnology can be incorporated into chemical and biological sensors to improve analytical performance, such as response time, sensitivity, selectivity, reliability, and accuracy. Based on the characteristics of the contaminants and the detection methods, nanotechnology can be applied in different ways in order to improve conventional techniques. Nanomaterials such as nanoparticles, nanorods, nanosheets, nanocomposites, nanotubes, and nanowires provide various functions for the immobilization and labeling of contaminants in electrochemical and optical detection. This review summarizes the recent advances in nanotechnology for detecting chemical and biological contaminations in the food supply chain.

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“…Immunoassays do make use of chemically attached or conjugated antigen or antibody labels like radio-isotopes, enzymes, chemiluminescent molecules or label-free methods. The latter group an example of which is Surface Plasmon Resonance (SPR) based Immunoassay, a technique which is very useful in the characterization of binding reactions in real-time, measuring endpoints such as antibody affinity, kinetics and crossreactivity (8).…”

Section: Types Of Immunoassaysmentioning

confidence: 99%

Interference in Immunoassays

MOGAKA

Karakuş

Bagriacik³

2023

Gazi Sağlık Bilimleri Dergisi

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Immunoassays are simple, efficient and reliable means for testing patient samples in the modern hospital. Their specificity and sensitivity have provided robust means by which detection of analytes can be achieved with ease. The underlying principle of antigen-antibody reactions forming a measurable complex exposes immunoassays to possible interference from proteins and other reactants with structural similarities to antibodies and or the analytes. Accounting for the sensitivity of the test and its reliability, the impact of interferents both endogenous like autoantibodies and exogenous factors like drugs, involved in the assay is worth investigating since the alternative would be producing unreliable results. Insights for developing tests for assessing discordant results following troubleshooting of immunoassays as possible remedial measures have been provided.

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Surface Plasmon Resonance–Based Immunoassays

Cited by 10 publications

References 59 publications

Controlling the Binding Efficiency of Surface Confined Antibodies through the Design of Mixed Self‐Assembled Monolayers

Controlling the Binding Efficiency of Surface Confined Antibodies through the Design of Mixed Self‐Assembled Monolayers

Recent Progress in Nanotechnology-Based Approaches for Food Monitoring

Interference in Immunoassays

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