Surface Plasmon Resonance Imaging by Holographic Enhanced Mapping

Abstract

We designed, constructed and tested a holographic surface plasmon resonance (HoloSPR) objective-based microscope for simultaneous amplitude-contrast and phase-contrast surface plasmon resonance imaging (SPRi). SPRi is a widely spread tool for label-free detection of changes in refractive index and concentration, as well as mapping of thin films. Currently, most of the SPR sensors rely on the detection of amplitude or phase changes of light. Despite the high sensitivities achieved so far, each technique alone has a limited detection range with optimal sensitivity. Here we use a high numerical aperture objective that avoids all the limitations due to the use of a prism-based configuration, yielding highly magnified and distortion-free images. Holographic reconstructions of SPR images and real-time kinetic measurements are presented to show the capability of HoloSPR to provide a versatile imaging method for high-throughput SPR detection complementary to conventional SPR techniques.

Subnanometer imaging and controlled dynamical patterning of thermocapillary driven deformation of thin liquid films

“…We hope that our work, which brings together plasmonic effects, SPRM and fluid dynamics, will stimulate the future development of specific optical systems aimed at studying TLD film processes and light-fluid interactions. In particular, other prominent SPRM variants that can be employed include the classical Kretschmann configuration to study the dynamics of TLD films of thicknesses below the grating depth, the holographic-based method 42 , which offers the possibility of simultaneous detection of reflectivity and phase changes in SPR images, and SPP confocal interferometry 43 , which offers superior noise performance and can improve the lateral resolution.…”

Label free imaging of cell‐substrate contacts by holographic total internal reflection microscopy

“…Total internal reflection of light can also be exploited for holographic measurements of interfaces. Digital Holography microscopy has the advantage of measuring both amplitude and phase information from the observed sample . The only experimental requirement is the formation of an interference pattern at the image plane with a reference beam.…”

Early detection of bacteria using SPR imaging and event counting: experiments withListeria monocytogenesandListeria innocua

“…31 SPRI systems are easily automated and compatible with real food samples. 9,19,32,33 Optical coupling can be achieved with gratings, 34 microscope objectives 35,36 or prisms, 19,32,37,38 with prism-based SPRI being the most frequently used conguration for the detection of bacteria. Prism-based SPRI systems are oen designed with a relatively wide eld of view (around 1 cm 2 ) for parallel measurements on a matrix of spots (spotted microarray) with detection levels as low as 100 pM in the case of DNA-DNA hybridization.…”