Surface Plasmon Resonance Imaging: What Next?

Spoto, Giuseppe; Minunni, Maria

doi:10.1021/jz301053n

Cited by 86 publications

(64 citation statements)

References 77 publications

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“…The signal enhancement produced when AuNPs are used in assays is a consequence of the large variation of the local dielectric constant caused by AuNPs [8]. In fact, the interaction between propagating and localized SPs established when nanoparticles are few nanometers far from the flat metal surface reinforces the local electric field [9–10]. The ultrasensitive detection of nucleic acids has been recently achieved by using streptavidin (SA)-conjugated AuNPs and SPR imaging (SPRI) [11–12].…”

Section: Introductionmentioning

confidence: 99%

Streptavidin-coated gold nanoparticles: critical role of oligonucleotides on stability and fractal aggregation

D’Agata

Palladino

Spoto³

2017

Beilstein J. Nanotechnol.

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Gold nanoparticles (AuNPs) exhibit unique properties that can be modulated through a tailored surface functionalization, enabling their targeted use in biochemical sensing and medical diagnostics. In particular, streptavidin-modified AuNPs are increasingly used for biosensing purposes. We report here a study of AuNPs surface-functionalized with streptavidin-biotinylated oligonucleotide, focussing on the role played by the oligonucleotide probes in the stabilization/destabilization of the functionalized nanoparticle dispersion. The behaviour of the modified AuNP dispersion as a consequence of the competitive displacement of the biotinylated oligonucleotide has been investigated and the critical role of displaced oligonucletides in triggering the quasi one-dimensional aggregation of nanoparticles is demonstrated for the first time. The thorough understanding of the fundamental properties of bioconjugated AuNPs is of great importance for the design of highly sensitive and reliable functionalized AuNP-based assays.

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Section: Introductionmentioning

confidence: 99%

Streptavidin-coated gold nanoparticles: critical role of oligonucleotides on stability and fractal aggregation

D’Agata

Palladino

Spoto³

2017

Beilstein J. Nanotechnol.

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“…22 Furthermore, the SPRi microarray requires less assay time than conventional SPR to analyze several replicates at once leading to high precision. 23,24 The hybridization of oligonucleotides with the SPR surface-immobilized capture probe results in an increase in the refractive index. The use of gold nanoparticles (AuNPs) has been shown to increase the reflectivity changes of a hybridization reaction due to surface plasmon signal enhancement.…”

Section: Introductionmentioning

confidence: 99%

Measuring ultra-low levels of nucleotide biomarkers using quartz crystal microbalance and SPR microarray imaging methods: A comparative analysis

Premaratne

Mubarak

Senavirathna

et al. 2017

Sensors and Actuators B: Chemical

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Circulating serum nucleotide biomarkers are useful indicators for early diagnosis of cancer, respiratory illnesses, and other deadly diseases. In this work, we compared detection performances of a quartz crystal microbalance (QCM), which is a mass sensor, with that of a surface plasmon resonance (SPR) microarray for an oligonucleotide mimic of a microRNA-21 biomarker. A surface immobilized capture oligonucleotide probe was used to hybridize with the target oligonucleotide (i.e., the microRNA-21 mimic) to facilitate selective detection. To obtain ultra-low femtomolar (fM) detection sensitivity, gold nanoparticles (50 nm) were conjugated with the target oligonucleotide. We achieved detection limits of 28and 47 fM for the target oligonucleotide by the QCM and SPRi microarray, respectively. We also conducted sample recovery studies and performed matrix effect analysis. Although the QCM had a lower detection limit, the microarray approach offered better throughput for analysis of up to 16 samples. We confirmed that the designed assay was selective for the target oligonucleotide and did not show signals for the control oligonucleotide with five mismatch sites relative to the target sequence. Combination of the QCM and microarray methods that utilize the same assay chemistry on gold are useful for overcoming clinical sample matrix effects and achieving ultra-low detection of small nucleotide biomarkers with quantitative insights.

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“…Over years, the technology of real-time and label-free detection has emerged as a useful and important tool for studying the affinity interactions, between nucleic acids, and nucleic acids and proteins [6]. A further advance in optical label-free sensing has been represented by SPR imaging (SPRi) [7], also known as SPR microscopy [8], first introduced by Rothenhäusler and Knoll in 1988 [9].…”

Section: Spr-based Biosensorsmentioning

confidence: 99%

Label free Affinity sensing: application to food analysis

Scarano¹,

Mariani²,

Minunni³

2016

ACTA IMEKO

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In this paper real-time, label free sensing principle based on Surface Plasmon Resonance (SPR) and gravimetric, i.e. Quartz Crystal Microbalance (QCM) are described and some applications to food analysis problems are reported. Affinity receptors are introduced and examples using antibodies, nucleic acid probes and biomimetic receptors i.e. molecular imprinted polymers (MIP) reported. In particualr pesticide, endotoxins, pathogens, Genetically Modified Organism (GMOs) detection and food origin analysis are reviewed.

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Surface Plasmon Resonance Imaging: What Next?

Cited by 86 publications

References 77 publications

Streptavidin-coated gold nanoparticles: critical role of oligonucleotides on stability and fractal aggregation

Streptavidin-coated gold nanoparticles: critical role of oligonucleotides on stability and fractal aggregation

Measuring ultra-low levels of nucleotide biomarkers using quartz crystal microbalance and SPR microarray imaging methods: A comparative analysis

Label free Affinity sensing: application to food analysis

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