Surface plasmon resonance immunoassay analysis of pituitary hormones in urine and serum samples

Treviño, Juan; Calle, A.; Rodrı́guez-Frade, José Miguel; Mellado, Mario; Lechuga, Laura M.

doi:10.1016/j.cca.2009.01.015

Cited by 60 publications

(34 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The limit of detection, 0.005 ng/mL, was calculated as three times the standard deviation of the signals provided (n010) by using 0.1 ng/mL hGH as the noise estimate. This detection limit is much lower than that reported using the SPRbased methodology, 4 ng/mL [8,9]. Moreover, a limit of quantification of 0.017 ng/mL was calculated as ten times the standard deviation (n010) of the estimate .This good analytical performance can be attributed to the combination of two important practical advantages: the immunoconjugate preconcentration provided by functionalized MBs and the high sensitivity inherent to the voltammetric transduction used.…”

Section: Resultsmentioning

confidence: 91%

“…The dynamic range of linearity comprised between 0.01 and 100 ng/mL (r 00.998) covering four orders of magnitude in hGH concentration, with the equation i(μA)05.7 logC +11.6 (r00.992, n08). The lower part of this linearity range involves much lower concentrations than those achieved with a SPR immunosensor, 18-542 ng/mL [9]. The limit of detection, 0.005 ng/mL, was calculated as three times the standard deviation of the signals provided (n010) by using 0.1 ng/mL hGH as the noise estimate.…”

Section: Resultsmentioning

confidence: 99%

“…More recently, enzyme-linked immunoassay (ELISA) was reported for the determination of hGH in whole blood spots in filter paper [7]. Regarding immunosensing techniques, surface plasmon resonance (SPR) methodologies were described for the determination of hGH in serum samples, achieving a detection limit of 4 ng/mL [8], as well as for the determination of various pituitary hormones in serum and urine, with a working range for hGH that extended from 33 to 1337 ng/mL [9]. On the other hand, an impedimetric immunosensor which involved the immobilization of a specific monoclonal antibody onto gold nanoparticle-coated 1,6-hexanedithiol-modified gold electrode was also reported, with a claimed detection limit of 0.64 pg/mL [10].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ultrasensitive determination of human growth hormone (hGH) with a disposable electrochemical magneto-immunosensor

et al. 2012

View full text Add to dashboard Cite

In this paper, an electrochemical magneto-immunosensor for the detection of human growth hormone (hGH) is described for the first time. The immunosensor involves the use of tosyl-activated magnetic microparticles (TsMBs) to covalently immobilize a monoclonal mAbhHG antibody. A sandwich-type immunoassay with a secondary pAbhGH antibody and anti-IgG labelled with alkaline phosphatase (anti-IgG-AP) was employed. TsMBs–mAbhGH–hGH–pAbhGH–anti-IgG-AP conjugates were deposited onto the surface of a screen-printed gold electrode using a small neodymium magnet, and electrochemical detection was performed by square-wave voltammetry upon the addition of 4-aminophenyl phosphate as the AP substrate. All the variables involved in the preparation of immunoconjugates and in the immunoassay protocol were optimized. A calibration curve for hGH was constructed with a linear range between 0.01 and 100 ng/mL (r = 0.998) and a limit of detection of 0.005 ng/mL. This value is nearly three orders of magnitude lower than that obtained using surface plasmon resonance (Treviño et al., Talanta 78:1011-1016, 2009). Furthermore, good repeatability, with RSD = 3% (n = 10) at the 1-ng/mL hGH level, was obtained. Cross-reactivity studies with other hormones demonstrated good selectivity. The magneto-immunosensor was applied to the analysis of human serum spiked with hGH at the 4- and 0.1-ng/mL levels. Mean recoveries of 96 ± 6% and 99 ± 2%, respectively, were obtained.

show abstract

Section: Resultsmentioning

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ultrasensitive determination of human growth hormone (hGH) with a disposable electrochemical magneto-immunosensor

et al. 2012

View full text Add to dashboard Cite

show abstract

“…The binding of the analyte causes a change in the refractive index, which can be detected without any signal amplification [62]. Matrix interferences are known to limit the suitability of SPR technology to analysis of clinical samples [63,64]. Nevertheless, SPR technology has been used for detecting analytes from saliva [63,65,66], urine, and serum [64], and it can be used in multianalyte assays [67,68].…”

Section: Label-free Methodsmentioning

confidence: 98%

Simple and inexpensive immunoassay-based diagnostic tests

Päkkilä

Soukka

2011

Bioanal Rev

View full text Add to dashboard Cite

Simple and inexpensive yet sensitive and robust diagnostic tests are critically needed for resource-poor settings to enable timely diagnosis and effective use of limited health care resources. Current tests are often too expensive, too slow, or have compromised clinical performance, and they often require health care professional to perform the test. In addition, most assays are not intended to be used in extreme environmental conditions, but their performance may be affected by high temperatures and humidity often encountered in resource-poor settings. This review provides an overview of current immunoassay technologies and their advantages and limitations with respect to their feasibility to resourcepoor settings. Future trends of immunoassay development for decentralized testing are also discussed. Homogeneous assays as such are out of the scope of this article because they are generally not yet sensitive enough or otherwise less feasible for inexpensive rapid diagnostic tests.

show abstract

“…13,14 This is particularly important for optical sensors, since the presence of blood cells in the sample can negatively affect the optical detection path and compromise accuracy. Detection technologies such as whispering gallerymode devices, 15 plasmon resonance devices, 16 and photonic crystals, 17 can benefit from the preremoval of nontargeted cells from whole blood to enhance the capture efficiency of targeted pathogens and proteins. In a clinical laboratory setting, plasma separation can simply be performed by centrifugation, whereas it remains a challenge at the POC, especially in resource-constrained settings due to lack of laboratory infrastructure.…”

Section: Introductionmentioning

confidence: 99%

Simple filter microchip for rapid separation of plasma and viruses from whole blood

Wang¹,

Chen²,

Huang³

et al. 2012

IJN

View full text Add to dashboard Cite

Sample preparation is a significant challenge for detection and sensing technologies, since the presence of blood cells can interfere with the accuracy and reliability of virus detection at the nanoscale for point-of-care testing. To the best of our knowledge, there is not an existing on-chip virus isolation technology that does not use complex fluidic pumps. Here, we presented a lab-on-a-chip filter device to isolate plasma and viruses from unprocessed whole blood based on size exclusion without using a micropump. We demonstrated that viruses (eg, HIV) can be separated on a filter-based chip (2-µm pore size) from HIV-spiked whole blood at high recovery efficiencies of 89.9% ± 5.0%, 80.5% ± 4.3%, and 78.2% ± 3.8%, for viral loads of 1000, 10,000 and 100,000 copies/mL, respectively. Meanwhile, 81.7% ± 6.7% of red blood cells and 89.5% ± 2.4% of white blood cells were retained on 2 µm pore-sized filter microchips. We also tested these filter microchips with seven HIV-infected patient samples and observed recovery efficiencies ranging from 73.1% ± 8.3% to 82.5% ± 4.1%. These results are first steps towards developing disposable point-of-care diagnostics and monitoring devices for resource-constrained settings, as well as hospital and primary care settings.

show abstract

Surface plasmon resonance immunoassay analysis of pituitary hormones in urine and serum samples

Cited by 60 publications

References 26 publications

Ultrasensitive determination of human growth hormone (hGH) with a disposable electrochemical magneto-immunosensor

Ultrasensitive determination of human growth hormone (hGH) with a disposable electrochemical magneto-immunosensor

Simple and inexpensive immunoassay-based diagnostic tests

Simple filter microchip for rapid separation of plasma and viruses from whole blood

Contact Info

Product

Resources

About